卵磷脂膽固醇脂酰轉(zhuǎn)移酶(LCAT)活性蛋白
Active Lecithin Cholesterol Acyltransferase (LCAT)
phosphatidylcholine-sterol O-acyltransferase; Phospholipid-cholesterol acyltransferase
- 編號UAPJ516Hu01
- 物種Homo sapiens (Human，人) 相同的名稱，不同的物種。
- 緩沖液成份PBS, pH7.4, containing 0.01% SKL, 5% Trehalose.
- 性狀凍干粉
- 純度> 90%
- 等電點5.8
- 應(yīng)用Cell?culture;?Activity?Assays.
- 下載 英文說明書 中文說明書
- 規(guī)格 10μg50μg 200μg 1mg 5mg
- 價格 ¥ 936 ¥ 2340 ¥ 4680 ¥ 14040 ¥ 35100
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產(chǎn)品包裝(模擬)
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產(chǎn)品包裝(模擬)
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Figure. SDS-PAGE
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通過ISO 9001、ISO 13485質(zhì)量體系認證
活性實驗
LCAT(Phosphatidylcholine-sterol acyltransferase) is an enzyme in the extracellular metabolism of plasma lipoproteins, which converts cholesterol and phosphatidylcholines(lecithins) to cholesteryl esters and lysophosphatidylcholines. It is reported that Apolipoprotein E (apoE) is the main LCAT activator in glia- conditioned media (GCM). Thus, a binding ELISA assay was conducted to detect the association of recombinant human LCAT with recombinant human apoE. Briefly, recombinant human LCAT were diluted serially in PBS with 0.01% BSA(pH 7.4). Duplicate samples of 100ul were then transferred to apoE-coated microtiter wells and incubated for 2h at 37°C. Wells were washed with PBST and incubated for 1 h with anti-LCAT pAb, then aspirated and washed 3 times. After incubation with HRP labelled secondary antibody, wells were aspirated and washed 3 times. With the addition of substrate solution, wells were incubated 15-25 minutes at 37°C. Finally, add 50μL stop solution to the wells and read at 450nm immediately. The binding activity of LCAT with apoE was shown in Figure 1 ,the EC50?for this effect is 0.036ug/mL.
用法
Reconstitute in 10mM PBS (pH7.4) to a concentration of 0.1-1.0 mg/mL. Do not vortex.
儲存
避免反復凍融。2-8°C不超過一個月，-80°C不超過12個月。
穩(wěn)定性
熱穩(wěn)定性以損失率顯示。損失率是由加速降解試驗決定，具體方法如下：在37°C孵育48小時，沒有顯著的降解或者沉淀產(chǎn)生。保質(zhì)期內(nèi)，在適當?shù)臈l件下存儲，損失率低于5%。
增值服務(wù)
相關(guān)產(chǎn)品
| 編號 | 適用物種：Homo sapiens (Human，人) | 應(yīng)用(僅供研究使用，不用于臨床診斷！) |
| URPJ516Hu01 | 卵磷脂膽固醇脂酰轉(zhuǎn)移酶(LCAT)重組蛋白 | Positive Control; Immunogen; SDS-PAGE; WB. |
| URPJ516Hu02 | 卵磷脂膽固醇脂酰轉(zhuǎn)移酶(LCAT)重組蛋白 | Positive Control; Immunogen; SDS-PAGE; WB. |
| UAPJ516Hu01 | 卵磷脂膽固醇脂酰轉(zhuǎn)移酶(LCAT)活性蛋白 | Cell?culture;?Activity?Assays. |
| UPAJ516Hu01 | 卵磷脂膽固醇脂酰轉(zhuǎn)移酶(LCAT)多克隆抗體 | WB; IHC; ICC; IP. |
| UPAJ516Hu02 | 卵磷脂膽固醇脂酰轉(zhuǎn)移酶(LCAT)多克隆抗體 | IHC |
| ULAJ516Hu71 | 卵磷脂膽固醇脂酰轉(zhuǎn)移酶(LCAT)多克隆抗體(生物素標記) | WB; IHC; ICC. |
| UMAJ516Hu21 | 卵磷脂膽固醇脂酰轉(zhuǎn)移酶(LCAT)單克隆抗體 | WB; IHC |
| UMAJ516Hu25 | 卵磷脂膽固醇脂酰轉(zhuǎn)移酶(LCAT)單克隆抗體 | IHC |
| UMAJ516Hu27 | 卵磷脂膽固醇脂酰轉(zhuǎn)移酶(LCAT)單克隆抗體 | IHC |
| UMAJ516Hu29 | 卵磷脂膽固醇脂酰轉(zhuǎn)移酶(LCAT)單克隆抗體 | WB |
| UMAJ516Hu24 | 卵磷脂膽固醇脂酰轉(zhuǎn)移酶(LCAT)單克隆抗體 | IHC; ICC/IF |
| UMAJ516Hu26 | 卵磷脂膽固醇脂酰轉(zhuǎn)移酶(LCAT)單克隆抗體 | WB |
| UMAJ516Hu28 | 卵磷脂膽固醇脂酰轉(zhuǎn)移酶(LCAT)單克隆抗體 | WB |
| UMAJ516Hu22 | 卵磷脂膽固醇脂酰轉(zhuǎn)移酶(LCAT)單克隆抗體 | WB; IHC |
| UMAJ516Hu23 | 卵磷脂膽固醇脂酰轉(zhuǎn)移酶(LCAT)單克隆抗體 | WB |
| USEJ516Hu | 卵磷脂膽固醇脂酰轉(zhuǎn)移酶(LCAT)檢測試劑盒(酶聯(lián)免疫吸附試驗法) | Enzyme-linked immunosorbent assay for Antigen Detection. |
| USCJ516Hu | 卵磷脂膽固醇脂酰轉(zhuǎn)移酶(LCAT)檢測試劑盒(化學發(fā)光免疫分析法) | Chemiluminescent immunoassay for Antigen Detection. |
| ULMJ516Hu | 卵磷脂膽固醇脂酰轉(zhuǎn)移酶(LCAT)等多因子檢測試劑盒(流式熒光發(fā)光法) | FLIA Kit for Antigen Detection. |
參考文獻
| 雜志 | 參考文獻 |
| Int J Rheum Dis | Plasma phospholipase, γ‐CEHC and antioxidant capacity in fibromyalgia [PubMed: 26585319] |
| Inflammation. | Alteration?of?Lysophosphatidylcholine-Related?Metabolic?Parameters?in the?Plasma?of?Mice?withExperimental?Sepsis. [pubmed:28028754] |
| British journal of cancer | A combined biomarker panel shows improved sensitivity for the early detection of ovarian cancer allowing the identification of the most aggressive type II tumours. [pubmed:28664912] |
| Journal?of?Agricultural?and?Food?Chemistry | Lipid-lowering effects of medium-chain triglyceride-enriched coconut oil in combination with licorice extracts in experimental hyperlipidemic mice [Pubmed: 30244576] |
| Experimental and Therapeutic Medicine | Identification and validation of differentially expressed proteins in serum of CSU patients with different duration of wheals using an iTRAQ labeling, 2D?LC?MS/MS [Doi: 10.3892/etm.2018.6818] |
| British Journal of Cancer | Diagnosis of epithelial ovarian cancer using a combined protein biomarker panel. [Pubmed: 31388184] |
| BIOMEDICINE & PHARMACOTHERAPY | The synergistic mechanism of total saponins and flavonoids in Notoginseng? Safflower pair against myocardial ischemia uncovered by an integrated?… [Pubmed: 32739736] |
| Biomedicine & Pharmacotherapy | Pharmacokinetics/pharmacometabolomics-pharmacodynamics reveals the synergistic mechanism of a multicomponent herbal formula, Baoyuan decoction??- [] |
| Cell Metab | Defects in a liver-bone axis contribute to hepatic osteodystrophy disease progression [Pubmed:35235775] |
