基質金屬蛋白酶2(MMP2)活性蛋白
Active Matrix Metalloproteinase 2 (MMP2)
CLG4; CLG4A; MMP-II; MONA; TBE-1; PEX; Gelatinase A; 72kDa Gelatinase; 72kDa Type IV Collagenase
- 編號UAPA100Hu02
- 物種Homo sapiens (Human，人) 相同的名稱，不同的物種。
- 緩沖液成份磷酸鹽緩沖液（pH7.4，含有 0.01% SKL, 1mM DTT, 5% Trehalose和Proclin300.）
- 性狀凍干粉
- 純度> 97%
- 等電點5.2
- 應用Cell?culture;?Activity?Assays.
- 下載 英文說明書 中文說明書
- 規(guī)格 10μg50μg 200μg 1mg 5mg
- 價格 ¥ 964 ¥ 2409 ¥ 4818 ¥ 14454 ¥ 36135
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產品包裝(模擬)
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產品包裝(模擬)
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Gene sequencing
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SDS-PAGE圖
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Figure. Western Blot; Sample: Recombinant MMP2, Human.
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通過ISO 9001、ISO 13485質量體系認證
活性實驗
Mechanism: MMP2 is a zinc-dependent enzymes capable of cleaving components of the extracellular matrix, which belongs to the matrix metalloproteinase (MMP) family. It is a gelatinase A, 72kDa type IV collagenase which can hydrolyze gelatin under certain conditions. Gelatin zymography is mainly used for the detection of the gelatinases, MMP-2 and MMP-9 and It is extremely sensitive because levels of 10pg of MMP-2 can already be detected. Briefly, various concentrations of MMP2 (500ng, 125ng, 63ng, 32ng, 16ng) were denatured by SDS loading buffer, electrophoresed through sodium dodecylsulphate-polyacrylamide gel (SDS-PAGE; 10% gels) containing gelatin (1mg/mL) with nonreducing conditions. After renaturation, incubation and CCB-stained, active MMP2 would hydrolyze gelatin nearby, which was indicated by the white binds on the gel. In this experiment we use heat-denatured MMP2 protein as negative control, and blood sample as positive control.
Result 1: Gelatin hydrolysis by recombinant human MMP2 was shown in figure 1.
用法
Reconstitute in PBS (pH7.4) to a concentration of 0.1-1.0 mg/mL. Do not vortex.
儲存
避免反復凍融。2-8°C不超過一個月，-80°C不超過12個月。
穩(wěn)定性
熱穩(wěn)定性以損失率顯示。損失率是由加速降解試驗決定，具體方法如下：在37°C孵育48小時，沒有顯著的降解或者沉淀產生。保質期內，在適當?shù)臈l件下存儲，損失率低于5%。
增值服務
相關產品
| 編號 | 適用物種：Homo sapiens (Human，人) | 應用(僅供研究使用，不用于臨床診斷！) |
| UEPA100Hu62 | 基質金屬蛋白酶2(MMP2)真核蛋白 | Positive Control; Immunogen; SDS-PAGE; WB. |
| UAPA100Hu62 | 基質金屬蛋白酶2(MMP2)活性蛋白 | Cell?culture;?Activity?Assays. |
| URPA100Hu01 | 基質金屬蛋白酶2(MMP2)重組蛋白 | Positive Control; Immunogen; SDS-PAGE; WB. |
| UAPA100Hu01 | 基質金屬蛋白酶2(MMP2)活性蛋白 | Cell?culture;?Activity?Assays. |
| UAPA100Hu61 | 基質金屬蛋白酶2(MMP2)活性蛋白 | Cell?culture;?Activity?Assays. |
| URPA100Hu03 | 基質金屬蛋白酶2(MMP2)重組蛋白 | Positive Control; Immunogen; SDS-PAGE; WB. |
| UEPA100Hu63 | 基質金屬蛋白酶2(MMP2)真核蛋白 | Positive Control; Immunogen; SDS-PAGE; WB. |
| UEPA100Hu61 | 基質金屬蛋白酶2(MMP2)真核蛋白 | Positive Control; Immunogen; SDS-PAGE; WB. |
| UAPA100Hu02 | 基質金屬蛋白酶2(MMP2)活性蛋白 | Cell?culture;?Activity?Assays. |
| URPA100Hu02 | 基質金屬蛋白酶2(MMP2)重組蛋白 | Positive Control; Immunogen; SDS-PAGE; WB. |
| UPAA100Hu02 | 基質金屬蛋白酶2(MMP2)多克隆抗體 | WB; IHC; ICC/IF |
| UPAA100Hu01 | 基質金屬蛋白酶2(MMP2)多克隆抗體 | WB; IHC |
| ULAA100Hu71 | 基質金屬蛋白酶2(MMP2)多克隆抗體(生物素標記) | WB; IHC; ICC. |
| ULAA100Hu81 | 基質金屬蛋白酶2(MMP2)多克隆抗體(異硫氰酸熒光素標記) | WB; IHC; ICC; IF. |
| UFAA100Hu41 | 抗基質金屬蛋白酶2(MMP2)多克隆抗體(藻紅素標記) | FCM |
| UMAA100Hu25 | 基質金屬蛋白酶2(MMP2)單克隆抗體 | WB |
| UMAA100Hu27 | 基質金屬蛋白酶2(MMP2)單克隆抗體 | ICC/IF |
| UMAA100Hu23 | 基質金屬蛋白酶2(MMP2)單克隆抗體 | WB |
| UMAA100Hu26 | 基質金屬蛋白酶2(MMP2)單克隆抗體 | WB |
| UMAA100Hu22 | 基質金屬蛋白酶2(MMP2)單克隆抗體 | WB; IHC |
| UMAA100Hu28 | 基質金屬蛋白酶2(MMP2)單克隆抗體 | WB |
| UMAA100Hu29 | 基質金屬蛋白酶2(MMP2)單克隆抗體 | WB |
| UMAA100Hu21 | 基質金屬蛋白酶2(MMP2)單克隆抗體 | IHC |
| UFAA100Hu02 | 抗基質金屬蛋白酶2(MMP2)單克隆抗體 | FCM |
| UFAA100Hu04 | 抗基質金屬蛋白酶2(MMP2)單克隆抗體 | FCM |
| UFAA100Hu06 | 抗基質金屬蛋白酶2(MMP2)單克隆抗體 | FCM |
| UFAA100Hu08 | 抗基質金屬蛋白酶2(MMP2)單克隆抗體 | FCM |
| USEA100Hu | 基質金屬蛋白酶2(MMP2)檢測試劑盒(酶聯(lián)免疫吸附試驗法) | Enzyme-linked immunosorbent assay for Antigen Detection. |
| USCA100Hu | 基質金屬蛋白酶2(MMP2)檢測試劑盒(化學發(fā)光免疫分析法) | Chemiluminescent immunoassay for Antigen Detection. |
| ULMA100Hu | 基質金屬蛋白酶2(MMP2)等多因子檢測試劑盒(流式熒光發(fā)光法) | FLIA Kit for Antigen Detection. |
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