肌纖蛋白(MYOC)活性蛋白
Active Myocilin (MYOC)
GLC1A; GPOA; JOAG; JOAG1; TIGR; Trabecular Meshwork Inducible Glucocorticoid Response; Myocilin 55 kDa subunit
- 編號(hào)UAPH586Mu61
- 物種Mus musculus (Mouse，小鼠) 相同的名稱，不同的物種。
- 緩沖液成份PBS, pH7.4, containing 5% Trehalose.
- 性狀凍干粉
- 純度> 80%
- 等電點(diǎn)6.1
- 應(yīng)用Cell?culture;?Activity?Assays.
- 下載 英文說明書 中文說明書
- 規(guī)格 10μg50μg 200μg 1mg 5mg
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產(chǎn)品包裝(模擬)
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產(chǎn)品包裝(模擬)
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Figure. SDS-PAGE
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通過ISO 9001、ISO 13485質(zhì)量體系認(rèn)證
活性實(shí)驗(yàn)
Myocilin (MYOC) is a secreted protein found in human aqueous humor (AH). Mutations of the MYOC gene are associated with primary open angle glaucoma (POAG), which is a complex disorder with a major heritable component. Myocilin stimulates cell migration that involves the activation of intergrin focal adhesion kinase (FAK)-serin/threonine kinase (AKT) signaling pathway. To test the effect of MYOC on cell proliferation, AGS cells were seeded into triplicate wells of 96-well plates and allowed to attach, replaced with various concentrations of recombinant mouse MYOC. After incubated for 72h, cells were observed by inverted microscope and cell proliferation was measured by Cell Counting Kit-8 (CCK-8). Briefly, 10 μl of CCK-8 solution was added to each well of the plate, then the absorbance at 450 nm was measured using a microplate reader after incubating the plate for 1-4 hours at 37 ℃. Cell viability was assessed by CCK-8 assay after incubation with recombinant mouse MYOC for 72h. The result was shown in Figure 1. It was obvious that MYOC significantly increased cell viability of AGS cells. The ED50 of recombinant mouse MYOC is 1.033μg/ml.
用法
Reconstitute in 10mM PBS (pH7.4) to a concentration of 0.1-1.0 mg/mL. Do not vortex.
儲(chǔ)存
避免反復(fù)凍融。2-8°C不超過一個(gè)月，-80°C不超過12個(gè)月。
穩(wěn)定性
熱穩(wěn)定性以損失率顯示。損失率是由加速降解試驗(yàn)決定，具體方法如下：在37°C孵育48小時(shí)，沒有顯著的降解或者沉淀產(chǎn)生。保質(zhì)期內(nèi)，在適當(dāng)?shù)臈l件下存儲(chǔ)，損失率低于5%。
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| 編號(hào) | 適用物種：Mus musculus (Mouse，小鼠) | 應(yīng)用(僅供研究使用，不用于臨床診斷！) |
| UAPH586Mu62 | 肌纖蛋白(MYOC)活性蛋白 | Cell?culture;?Activity?Assays. |
| UEPH586Mu61 | 肌纖蛋白(MYOC)真核蛋白 | Positive Control; Immunogen; SDS-PAGE; WB. |
| UEPH586Mu62 | 肌纖蛋白(MYOC)真核蛋白 | Positive Control; Immunogen; SDS-PAGE; WB. |
| URPH586Mu01 | 肌纖蛋白(MYOC)重組蛋白 | Positive Control; Immunogen; SDS-PAGE; WB. |
| UAPH586Mu61 | 肌纖蛋白(MYOC)活性蛋白 | Cell?culture;?Activity?Assays. |
