紅細(xì)胞生成素(EPO)單克隆抗體
Monoclonal Antibody to Erythropoietin (EPO)
EP; Epoetin; Erythropoetin; Hematopoietin; Hemopoietin
- 編號UMAA028Hu22
- 物種Homo sapiens (Human，人) 相同的名稱，不同的物種。
- 來源單抗制備
- 宿主小鼠
- 效價(克隆號)C3
- Ig亞型 IgG1 Kappa
- 純化方式蛋白A + 蛋白G親和純化
- 標(biāo)記物無標(biāo)記物
- 免疫原 EPA028Hu61-紅細(xì)胞生成素(EPO)真核蛋白
- 緩沖液成份0.01M 磷酸鹽緩沖液（pH7.4，containing 0.05% Proclin-300，50% glycerol）
- 性狀液體
- 濃度1mg/ml
- 且適物種Rattus norvegicus (Rat，大鼠)
- 應(yīng)用WB; IHC
如果抗體需用于流式細(xì)胞術(shù)，請參見流式抗體。 - 下載 英文說明書 中文說明書
- 規(guī)格 20μl100μl 200μl 1ml 10ml
- 價格 ¥ 270 ¥ 630 ¥ 900 ¥ 2250 ¥ 9000
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產(chǎn)品包裝(模擬)
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產(chǎn)品包裝(模擬)
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DAB staining on IHC-P; Samples: Human Stomach Tissue; Primary Ab: 30μg/ml Mouse Anti-Human EPO Antibody Second Ab: 2μg/mL?HRP-Linked Caprine Anti-Mouse IgG Polyclonal Antibody (Catalog: SAA544Mu19)
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DAB staining on IHC-P;
Samples: Human Kidney Tissue;
Primary Ab: 30μg/ml Mouse Anti-Human EPO Antibody
Second Ab: 2μg/mL?HRP-Linked Caprine Anti-Mouse IgG Polyclonal Antibody
(Catalog: SAA544Mu19) -
DAB staining on IHC-P;
Samples: Human Thyroid cancer Tissue;
Primary Ab: 30μg/ml Mouse Anti-Human EPO Antibody
Second Ab: 2μg/mL?HRP-Linked Caprine Anti-Mouse IgG Polyclonal Antibody
(Catalog: SAA544Mu19) -
DAB staining on IHC-P;
Sample: Human Kidney Tissue
Primary Ab: 30μg/ml Mouse Anti-Human EPO Antibody
Control: Used PBS instead of primary antibody
Second Ab: 2μg/ml HRP-Linked Caprine Anti-Mouse IgG Polyclonal Antibody
(Catalog: SAA544Mu19)
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Western Blot; Sample: Rat Liver lysate; Primary Ab: 5μg/ml Mouse Anti-Human EPO Antibody Second Ab: 0.2μg/mL?HRP-Linked Caprine Anti-Mouse IgG Polyclonal Antibody (Catalog: SAA544Mu19)
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通過ISO 9001、ISO 13485質(zhì)量體系認(rèn)證
特異性
該抗體是針對EPO的小鼠單克隆抗體。在免疫組織化學(xué)染色和免疫印跡實驗中能識別EPO。
用法
Western blotting: 0.01-2μg/mL;
Immunohistochemistry: 5-20μg/mL;
Immunocytochemistry: 5-20μg/mL;
Optimal working dilutions must be determined by end user.
儲存
經(jīng)常使用則4°C保存。-20°C保存不超過兩年。避免反復(fù)凍融。
穩(wěn)定性
熱穩(wěn)定性以損失率顯示。損失率是由加速降解試驗決定，具體方法如下：在37°C孵育48小時，沒有顯著的降解或者沉淀產(chǎn)生。保質(zhì)期內(nèi)，在適當(dāng)?shù)臈l件下存儲，損失率低于5%。
贈品
增值服務(wù)
相關(guān)產(chǎn)品
| 編號 | 適用物種：Homo sapiens (Human，人) | 應(yīng)用(僅供研究使用，不用于臨床診斷！) |
| UAPA028Hu61 | 紅細(xì)胞生成素(EPO)活性蛋白 | Cell?culture;?Activity?Assays. |
| UEPA028Hu61 | 紅細(xì)胞生成素(EPO)真核蛋白 | Positive Control; Immunogen; SDS-PAGE; WB. |
| URPA028Hu01 | 紅細(xì)胞生成素(EPO)重組蛋白 | Positive Control; Immunogen; SDS-PAGE; WB. |
| URPA028Hu04 | 紅細(xì)胞生成素(EPO)重組蛋白 | Positive Control; Immunogen; SDS-PAGE; WB. |
| UAPA028Hu01 | 紅細(xì)胞生成素(EPO)活性蛋白 | Cell?culture;?Activity?Assays. |
| UAPA028Hu62 | 紅細(xì)胞生成素(EPO)活性蛋白 | Cell?culture;?Activity?Assays. |
| UPAA028Hu06 | 紅細(xì)胞生成素(EPO)多克隆抗體 | WB; IHC |
| UPAA028Hu01 | 紅細(xì)胞生成素(EPO)多克隆抗體 | IHC |
| ULAA028Hu71 | 紅細(xì)胞生成素(EPO)多克隆抗體(生物素標(biāo)記) | WB; IHC; ICC. |
| UMAA028Hu23 | 紅細(xì)胞生成素(EPO)單克隆抗體 | WB |
| UMAA028Hu26 | 紅細(xì)胞生成素(EPO)單克隆抗體 | WB |
| UMAA028Hu21 | 紅細(xì)胞生成素(EPO)單克隆抗體 | IHC |
| UMAA028Hu24 | 紅細(xì)胞生成素(EPO)單克隆抗體 | IHC |
| UMAA028Hu25 | 紅細(xì)胞生成素(EPO)單克隆抗體 | IHC |
| UMAA028Hu22 | 紅細(xì)胞生成素(EPO)單克隆抗體 | WB; IHC |
| USEA028Hu | 紅細(xì)胞生成素(EPO)檢測試劑盒(酶聯(lián)免疫吸附試驗法) | Enzyme-linked immunosorbent assay for Antigen Detection. |
| USCA028Hu | 紅細(xì)胞生成素(EPO)檢測試劑盒(化學(xué)發(fā)光免疫分析法) | Chemiluminescent immunoassay for Antigen Detection. |
| ULMA028Hu | 紅細(xì)胞生成素(EPO)等多因子檢測試劑盒(流式熒光發(fā)光法) | FLIA Kit for Antigen Detection. |
| UKSA028Hu01 | 紅細(xì)胞生成素(EPO)檢測試劑盒DIY材料(酶聯(lián)免疫吸附試驗法) | Main materials for "Do It (ELISA Kit) Yourself". |
參考文獻(xiàn)
| 雜志 | 參考文獻(xiàn) |
| Endocrinology | Prenatal Glucocorticoid Overexposure Causes Permanent Increases in Renal Erythropoietin Expression and Red Blood Cell Mass in the Rat Offspring [Endo: source] |
| Journal of Ethnopharmacology | Inhibitory effect of polysaccharides isolated from Angelica sinensis on hepcidin expression [PubMed: 21333724] |
| PLOS ONE | CD98 Positive Eosinophils Contribute to T Helper 1 Pattern Inflammation [PlosOne: Source] |
| The Journal of Clinical Endocrinology & Metabolism | Alterations of Circulating Endothelial Cell and Endothelial Progenitor Cell Counts around the Ovulation [PubMed: 22948762] |
| Experimental & Molecular Medicine | Long-term and stable correction of uremic anemia by intramuscular injection of plasmids containing hypoxia-regulated system of erythropoietin expression [PubMed: PMC3509184] |
| Biochem Biophys Res Commun. | Reevaluation of erythropoietin production by the nephron [Pubmed:24832733] |
| Nutrition. | Carbohydrate and glutamine supplementation modulates the Th1/Th2 balance after exercise performed at a simulated altitude of 4500 m. [Pubmed:2528040] |
| J Appl Physiol (1985). | Decreased plasma soluble erythropoietin receptor in high-altitude excessive erythrocytosis and Chronic Mountain Sickness [Pubmed:25324511] |
| Expert Rev Proteomics | Plasma hepcidin in early-stage breast cancer patients: no relationship with interleukin-6, erythropoietin and erythroferrone [PubMed: 26496240] |
| Oncotarget | KIAA0101 is associated with human renal cell carcinoma proliferation and migration induced by erythropoietin [pubmed:26575329] |
| international journal of molecular sciences | Expression of Iron-Related Proteins Differentiate Non-Cancerous and Cancerous Breast Tumors. [pubmed:28216608] |
| Veterinary Clinical Pathology | Occult gastrointestinal bleeding is a common finding in dogs with chronic kidney disease [pubmed:28186645] |
| IOS Press Content Library | An Intranasal Formulation of Erythropoietin (Neuro-EPO) Prevents Memory Deficits and Amyloid Toxicity in the APPSwe Transgenic Mouse Model of Alzheimer's … [articles:journal-of-alzheimers-disease] |
| Oxidative Medicine and Cellular Longevity | Acidic Polysaccharide from Angelica sinensis Reverses Anemia of Chronic Disease Involving the Suppression of Inflammatory Hepcidin and NF-κB Activation [pubmed:29147463] |
| PLoS?Neglected?Tropical?Diseases | Functional and phenotypic evaluation of eosinophils from patients with the acute form of paracoccidioidomycosis [pubmed:28489854] |
| Neurochemistry International | Effects of erythropoietin on astrocytes and brain endothelial cells in primary culture during anoxia depend on simultaneous signaling by other cytokines and on duration of anoxia [S0197018617304783] |
| Pesquisa Veterinária Brasileira | Relation between anaemia and bone marrow features and serum erythropoietin in dogs with chronic kidney disease [10.1590/s0100-736x2017000600011?] |
| Medical Journal Armed Forces India | Study of acute hypoxia markers in healthy subjects: Utility in post-crash investigation [10.1016/j.mjafi.2017.04.003] |
| Neurochemistry International | Effects of erythropoietin on astrocytes and brain endothelial cells in primary culture during anoxia depend on simultaneous signaling by other cytokines and on?… [Pubmed:29180303] |
| Clinical and Experimental?Medicine | Increased NGAL level associated with iron store in chronic kidney disease with anemia [Pubmed:29909502] |
| Bulletin?of Experimental?Biology?and?Medicine | Signal Mechanism of the Protective Effect of Combined Preconditioning by Amtizole and Moderate Hypoxia [Pubmed:29308565] |
| Bulletin?of Experimental?Biology?and?Medicine | Expression of Hif-1α, Nf-κb, and Vegf Genes in the Liver and Blood Serum Levels of HIF-1α, Erythropoietin, VEGF, TGF-β, 8-Isoprostane, and Corticosterone in Wistar Rats with High and Low Resistance to Hypoxia. Corticosterone in?… [Pubmed: 30353332] |
| FASEB?JOURNAL | High-altitude hypoxia decreases plasma erythropoietin soluble receptor (sEpoR) concentration in humans [] |
| Journal of Functional Foods | Dietary gelatin enhances non-heme iron absorption possibly via regulation of systemic iron homeostasis in rats [] |
| 博士論文 | PHYSIOLOGICAL ADAPTATIONS TO HEAT ACCLIMATION; REPERCUSSIONS ON CYCLING PERFORMANCE [] |
| Nutrition | Effects of carbohydrate and glutamine supplementation on cytokine production by monocytes after exercise in hypoxia: a crossover, randomized and double-blind?… [Pubmed: 31743809] |
| BRITISH JOURNAL OF HAEMATOLOGY | Disordered serum erythroferrone and hepcidin levels as indicators of the spontaneous abortion occurrence during early pregnancy in humans [Pubmed: 32866306] |
| JCI Insight. | Increased FGF-23 levels are linked to ineffective erythropoiesis and impaired bone mineralization in myelodysplastic syndromes [Pubmed: 32759495] |
| Blood Cells, Molecules, and Diseases | EPAS1 regulates proliferation of erythroblasts in chronic mountain sickness [Pubmed: 32470757] |
| Int J Endocrinol | miR663 Prevents Epo Inhibition Caused by TNF-Alpha in Normoxia and Hypoxia [34367277] |
| J Mater Sci Mater Med | Loading of erythropoietin on biphasic calcium phosphate bioceramics promotes osteogenesis and angiogenesis by regulating EphB4/EphrinB2 molecules [Pubmed:35072831] |
| Effect of arsenate on erythropoietin production and autophagy induction in HepG2 cells [] |
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