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音猬因子(SHH)多克隆抗體

Polyclonal Antibody to Hedgehog Homolog, Sonic (SHH)

HHG1; HLP3; HPE3; SMMCI; SHHN; SHH-N

信號(hào)轉(zhuǎn)導(dǎo)

編號(hào)UPAB831Hu01
物種Homo sapiens (Human，人) 相同的名稱，不同的物種。
- 所有
- 人
- 小鼠
- 大鼠
- 豚鼠
- 家兔
- 獼猴
- 山羊
- 綿羊
- 馬
- 牛
- 豬
- 雞
- 狗
- 其他物種
- 多物種
- 泛物種
來源多抗制備
宿主兔
效價(jià)-
Ig類型 IgG
純化方式抗原特異性親和純化
標(biāo)記物無標(biāo)記物
免疫原 RPB831Hu01-音猬因子(SHH)重組蛋白
緩沖液成份0.01M 磷酸鹽緩沖液（pH7.4，containing 0.05% Proclin-300，50% glycerol）
性狀液體
濃度500μg/mL
且適物種Mus musculus (Mouse，小鼠), Rattus norvegicus (Rat，大鼠)
應(yīng)用WB; IHC; ICC/IF
如果抗體需用于流式細(xì)胞術(shù)，請(qǐng)參見流式抗體。
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規(guī)格 20μl100μl 200μl 1ml 10ml
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產(chǎn)品包裝(模擬)
產(chǎn)品包裝(模擬)

DAB staining on IHC-P; Samples: Human Liver Tissue; Primary Ab: 10μg/ml Rabbit Anti-Human SHH Antibody Second Ab: 2μg/mL?HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody (Catalog: SAA544Rb19)
DAB staining on IHC-P;
Samples: Human Liver cancer Tissue;
Primary Ab: 10μg/ml Rabbit Anti-Human SHH Antibody
Second Ab: 2μg/mL?HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
(Catalog: SAA544Rb19)
DAB staining on IHC-P;
Samples: Human Brain Tissue;
Primary Ab: 10μg/ml Rabbit Anti-Human SHH Antibody
Second Ab: 2μg/mL?HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
(Catalog: SAA544Rb19)
AF488 staining on IF;
Sample: HepG2 cell
Primary Ab: 20μg/ml Rabbit Anti-Human SHH Antibody
Second Ab: 2μg/ml AF488-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
(Catalog: SAA544Rb11)

Western Blot; Sample: PANC1 cell lysate Primary Ab: 0.5μg/ml Rabbit Anti-Human SHH Antibody Second Ab: 0.2μg/ml HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody (Catalog: SAA544Rb19)
Western Blot; Samples: Lane1: Mouse Liver lysate; Lane2: Mouse Kidney lysate; Lane3: Rat Liver lysate; Lane4: Rat Kidney lysate;
Primary Ab: 0.5μg/ml Rabbit Anti-Human SHH Antibody
Second Ab: 0.2μg/ml HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
(Catalog: SAA544Rb19)

通過ISO 9001、ISO 13485質(zhì)量體系認(rèn)證

特異性

該抗體是針對(duì)SHH的兔多克隆抗體。在免疫組織化學(xué)染色和免疫印跡實(shí)驗(yàn)中能識(shí)別SHH。

用法

Western blotting: 1-5ug/ml
Immunocytochemistry in formalin fixed cells: 5-20ug/ml
Immunohistochemistry in formalin fixed frozen section: 5-20ug/ml
Immunohistochemistry in paraffin section: 5-20ug/ml
Enzyme-linked Immunosorbent Assay: 0.05-2ug/ml
Optimal working dilutions must be determined by end user.

儲(chǔ)存

經(jīng)常使用則4°C保存。-20°C保存不超過兩年。避免反復(fù)凍融。

穩(wěn)定性

熱穩(wěn)定性以損失率顯示。損失率是由加速降解試驗(yàn)決定，具體方法如下：在37°C孵育48小時(shí)，沒有顯著的降解或者沉淀產(chǎn)生。保質(zhì)期內(nèi)，在適當(dāng)?shù)臈l件下存儲(chǔ)，損失率低于5%。

贈(zèng)品

增值服務(wù)

抗體標(biāo)記定制服務(wù)
Protein-A/G純化柱
 細(xì)胞和組織染色液
 抗體陽(yáng)性對(duì)照品
 組織/切片定制服務(wù)
磷酸化抗體定制服務(wù)
免疫印跡(WB)實(shí)驗(yàn)服務(wù)
免疫組織化學(xué)(IHC)實(shí)驗(yàn)服務(wù)
免疫細(xì)胞化學(xué)(ICC)實(shí)驗(yàn)服務(wù)
流式細(xì)胞術(shù)(FCM)實(shí)驗(yàn)服務(wù)
免疫沉淀(IP)實(shí)驗(yàn)服務(wù)
免疫熒光(IF)實(shí)驗(yàn)服務(wù)
緩沖液
 DAB顯色試劑盒
 SABC試劑盒
 長(zhǎng)臂生物素標(biāo)記試劑盒
 實(shí)時(shí)熒光定量PCR實(shí)驗(yàn)服務(wù)

相關(guān)產(chǎn)品

編號(hào)	適用物種：Homo sapiens (Human，人)	應(yīng)用(僅供研究使用，不用于臨床診斷！)
URPB831Hu01	音猬因子(SHH)重組蛋白	Positive Control; Immunogen; SDS-PAGE; WB.
UEPB831Hu61	音猬因子(SHH)真核蛋白	Positive Control; Immunogen; SDS-PAGE; WB.
UAPB831Hu01	音猬因子(SHH)活性蛋白	Cell?culture;?Activity?Assays.
UAPB831Hu61	音猬因子(SHH)活性蛋白	Cell?culture;?Activity?Assays.
UPAB831Hu01	音猬因子(SHH)多克隆抗體	WB; IHC; ICC/IF
UMAB831Hu22	音猬因子(SHH)單克隆抗體	WB; IHC
UMAB831Hu21	音猬因子(SHH)單克隆抗體	WB; IHC
UMAB831Hu24	音猬因子(SHH)單克隆抗體	WB
URAB831Hu21	音猬因子(SHH)重組抗體	WB; IF; ICC; IHC; IP; FCM.
UMAB831Hu23	音猬因子(SHH)單克隆抗體	IHC
USEB831Hu	音猬因子(SHH)檢測(cè)試劑盒(酶聯(lián)免疫吸附試驗(yàn)法)	Enzyme-linked immunosorbent assay for Antigen Detection.
UMEB831Hu	音猬因子(SHH)檢測(cè)試劑盒(酶聯(lián)免疫吸附試驗(yàn)法,小樣本)	Enzyme-linked immunosorbent assay for Antigen Detection.
ULMB831Hu	音猬因子(SHH)等多因子檢測(cè)試劑盒(流式熒光發(fā)光法)	FLIA Kit for Antigen Detection.

參考文獻(xiàn)

雜志	參考文獻(xiàn)
United States Patent Application	Compositions and Methods for Diagnosing Lung Cancer [y2016:0077095.html]
Annals of the Rheumatic Diseases	Elevated serum levels of sonic hedgehog are associated with fibrotic and vascular manifestations in systemic sclerosis [pubmed:28495672]

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