硫氧化還原蛋白(Trx)多克隆抗體
Polyclonal Antibody to Thioredoxin (Trx)
TXN; ADF; SASP; TRDX; TRX1; ATL-derived factor; Surface-associated sulphydryl protein
- 編號UPAA702Hu01
- 物種Homo sapiens (Human，人) 相同的名稱，不同的物種。
- 來源多抗制備
- 宿主兔
- 效價-
- Ig類型 IgG
- 純化方式抗原特異性親和純化
- 標記物無標記物
- 免疫原 RPA702Hu01-硫氧化還原蛋白(Trx)重組蛋白
- 緩沖液成份0.01M 磷酸鹽緩沖液（pH7.4，containing 0.05% Proclin-300，50% glycerol）
- 性狀液體
- 濃度0.26mg/mL
- 且適物種-
- 應用WB; ICC/IF; FCM
如果抗體需用于流式細胞術(shù)，請參見流式抗體。 - 下載 英文說明書 中文說明書
- 規(guī)格 20μl100μl 200μl 1ml 10ml
- 價格 ¥ 284 ¥ 664 ¥ 948 ¥ 2370 ¥ 9480
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產(chǎn)品包裝(模擬)
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產(chǎn)品包裝(模擬)
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FITC staining on IF; Samples: Human MCF7 cell; Primary Ab: 20μg/ml Rabbit Anti-Human Trx Antibody Second Ab: 1.5μg/ml FITC-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody (Catalog: SAA544Rb18)
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Figure. Western Blot; Sample: Recombinant Trx, Human.
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Western Blot; Samples: Lane1: Hela cell lysate; Lane2: HepG2 cell lysate; Lane3: A549 cell lysate;
Primary Ab: 0.2μg/ml Rabbit Anti-Human Trx Antibody
Second Ab: 0.2μg/ml HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
(Catalog: SAA544Rb19) -
HepG2 human hepatocellular carcinoma cell line was stained with rabbit Anti-human Trx Polyclonal Antibody (Catalog # PAA702Hu01, filled red histogram) or Isotype control antibody (Catalog # IS067-Rb01, filled green histogram), followed by APC-conjugated Anti-rabbit IgG Secondary Antibody (Catalog # SAA544Rb15). Cells were fixed with 4% paraformaldehyde and permeabilized with 0.1% Triton X-100.
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Human A549 cell was fixed with 2% paraformaldehyde (10 min) , permeabilised with 0.1% BSA-Triton X-100,then stained with 20μg/ml rabbit Anti-human Trx Polyclonal Antibody (Catalog PAA702Hu01, red histogram) or Isotype control antibody (Catalog IS067-Rb01, green histogram), followed by 1μg/ml FITC-conjugated Anti-rabbit IgG Secondary Antibody (Catalog SAA544Rb18).
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通過ISO 9001、ISO 13485質(zhì)量體系認證
特異性
該抗體是針對Trx的兔多克隆抗體。在免疫組織化學染色和免疫印跡實驗中能識別Trx。
用法
Western blotting: 0.01-2μg/mL;
Immunofluorescence: 5-20μg/mL;
Flow cytometry: 20μg/mL;
Optimal working dilutions must be determined by end user.
儲存
經(jīng)常使用則4°C保存。-20°C保存不超過兩年。避免反復凍融。
穩(wěn)定性
熱穩(wěn)定性以損失率顯示。損失率是由加速降解試驗決定，具體方法如下：在37°C孵育48小時，沒有顯著的降解或者沉淀產(chǎn)生。保質(zhì)期內(nèi)，在適當?shù)臈l件下存儲，損失率低于5%。
贈品
增值服務
相關(guān)產(chǎn)品
| 編號 | 適用物種：Homo sapiens (Human，人) | 應用(僅供研究使用，不用于臨床診斷！) |
| URPA702Hu01 | 硫氧化還原蛋白(Trx)重組蛋白 | Positive Control; Immunogen; SDS-PAGE; WB. |
| UPAA702Hu01 | 硫氧化還原蛋白(Trx)多克隆抗體 | WB; ICC/IF; FCM |
| UFAA702Hu01 | 抗硫氧化還原蛋白(Trx)多克隆抗體 | FCM |
| ULAA702Hu81 | 硫氧化還原蛋白(Trx)多克隆抗體(異硫氰酸熒光素標記) | WB; IHC; ICC; IF. |
| ULAA702Hu71 | 硫氧化還原蛋白(Trx)多克隆抗體(生物素標記) | WB; IHC; ICC. |
| UMAA702Hu25 | 硫氧化還原蛋白(Trx)單克隆抗體 | ICC/IF; FCM |
| UMAA702Hu27 | 硫氧化還原蛋白(Trx)單克隆抗體 | ICC/IF |
| UMAA702Hu26 | 硫氧化還原蛋白(Trx)單克隆抗體 | WB; ICC/IF |
| UMAA702Hu29 | 硫氧化還原蛋白(Trx)單克隆抗體 | ICC/IF |
| UMAA702Hu28 | 硫氧化還原蛋白(Trx)單克隆抗體 | WB; ICC/IF |
| UMAA702Hu22 | 硫氧化還原蛋白(Trx)單克隆抗體 | WB |
| UMAA702Hu21 | 硫氧化還原蛋白(Trx)單克隆抗體 | WB; ICC/IF |
| UMAA702Hu23 | 硫氧化還原蛋白(Trx)單克隆抗體 | WB; IHC; ICC/IF |
| UMAA702Hu24 | 硫氧化還原蛋白(Trx)單克隆抗體 | WB; IHC; ICC/IF |
| UFAA702Hu08 | 抗硫氧化還原蛋白(Trx)單克隆抗體 | Flow cytometry. |
| UFAA702Hu06 | 抗硫氧化還原蛋白(Trx)單克隆抗體 | Flow cytometry. |
| UFAA702Hu04 | 抗硫氧化還原蛋白(Trx)單克隆抗體 | Flow cytometry. |
| UFAA702Hu02 | 抗硫氧化還原蛋白(Trx)單克隆抗體 | Flow cytometry. |
| USEA702Hu | 硫氧化還原蛋白(Trx)檢測試劑盒(酶聯(lián)免疫吸附試驗法) | Enzyme-linked immunosorbent assay for Antigen Detection. |
| USCA702Hu | 硫氧化還原蛋白(Trx)檢測試劑盒(化學發(fā)光免疫分析法) | Chemiluminescent immunoassay for Antigen Detection. |
| ULMA702Hu | 硫氧化還原蛋白(Trx)等多因子檢測試劑盒(流式熒光發(fā)光法) | FLIA Kit for Antigen Detection. |
參考文獻
| 雜志 | 參考文獻 |
| Human Reproduction | The roles of thioredoxin and thioredoxin-binding protein-2 in endometriosis [OxfordJournals: source] |
| Exp Ther Med. | Altered glutamate cysteine ligase activity in peripheral blood mononuclear cells from patients with systemic lupus erythematosus [Pubmed:Pmc4061188] |
| Reproduction | Alteration in the intrafollicular thiol–redox system in infertile women with endometriosis [Pubmed:25376627] |
| Free Radical Research | Thalassemia major patients using iron chelators showed a reduced plasma thioredoxin level and reduced thioredoxin reductase activity, despite elevated oxidative stress [Pubmed:25564095] |
| Antioxidants | Comparison of the Pulmonary Oxidative Stress Caused by Intratracheal Instillation and Inhalation of NiO Nanoparticles when Equivalent Amounts of NiO Are Retained in the Lung. [Pubmed:26797643] |
| FASEB?Journal | Relationship between aortic wall oxidative stress/proteolytic enzyme expression and intraluminal thrombus thickness indicates a novel pathomechanism in the?… [Pubmed: 30351992] |
| World?Journal?of?Gastrointestinal?Oncology | Redox, immune and genetic biomarker system for personalized treatments in colorectal cancer [Doi: 10.4251/wjgo.v11.i2.117] |
| Nutrients | The Beneficial Effects of Lactobacillus plantarum PS128 on High-Intensity, Exercise-Induced Oxidative Stress, Inflammation, and Performance in Triathletes [Pubmed: 30736479] |
| European Journal of Pharmacology | Nilotinib enhances β-islets integrity and secretory functions in a rat model of STZ-induced diabetes mellitus [Pubmed: 31351037] |
| Journal of Clinical Medicine | Damage-Associated Molecular Patterns and Th-Cell-Related Cytokines Released after Progressive Effort [Pubmed: 32210109] |
| HISTOCHEMISTRY AND CELL BIOLOGY | Thioredoxin 1 is upregulated in the bone and bone marrow following experimental myocardial infarction: evidence for a remote organ response [Pubmed: 33161477] |
| Journal of Cosmetic Dermatology | Examination of androgenetic alopecia with serum biomarkers [Pubmed: 32969583] |
| Heliyon | Hepatoprotective Effect of Saccharomyces Cervisciae Cell Wall Extract against Thioacetamide-induced Liver Fibrosis in Rats [34159266] |
| Hum Exp Toxicol | Carvacrol hinders the progression of hepatic fibrosis via targeting autotaxin and thioredoxin in thioacetamide-induced liver fibrosis in rat [34155936] |
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