促腎上腺皮質(zhì)激素(ACTH)檢測(cè)試劑盒(化學(xué)發(fā)光免疫分析法)
CLIA Kit for Adrenocorticotropic Hormone (ACTH)
Corticotropin; Adrenocorticotropin
- 編號(hào)UCCA836Hu
- 物種Homo sapiens (Human，人) 相同的名稱，不同的物種。
- 實(shí)驗(yàn)方法競(jìng)爭(zhēng)抑制
- 反應(yīng)時(shí)長(zhǎng)2h
- 檢測(cè)范圍3.9-1,000pg/mL
- 靈敏度最小可檢測(cè)劑量小于等于1.5pg/mL.
- 樣本類型serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
- 下載 英文說明書 中文說明書
- 規(guī)格 48T96T 96T*5 96T*10 96T*100
- 價(jià)格 ¥ 1764 ¥ 2520 ¥ 11340 ¥ 21420 ¥ 176400
- 欲了解實(shí)際交易價(jià)格和更多情況，請(qǐng)與當(dāng)?shù)亟?jīng)銷商聯(lián)系!
-
產(chǎn)品包裝(模擬)
-
產(chǎn)品包裝(模擬)
-
實(shí)驗(yàn)結(jié)果圖
-
標(biāo)準(zhǔn)曲線圖
-
通過ISO 9001、ISO 13485質(zhì)量體系認(rèn)證
特異性
本試劑盒用于檢測(cè)促腎上腺皮質(zhì)激素(ACTH)，經(jīng)檢測(cè)與其它相似物質(zhì)無明顯交叉反應(yīng)。
由于受到技術(shù)及樣本來源的限制，不可能完成對(duì)所有相關(guān)或相似物質(zhì)交叉反應(yīng)檢測(cè)，因此本試劑盒有可能與未經(jīng)檢測(cè)的其它物質(zhì)有交叉反應(yīng)。
回收率
分別于定值血清及血漿樣本中加入一定量的促腎上腺皮質(zhì)激素(ACTH)（加標(biāo)樣品），重復(fù)測(cè)定并計(jì)算其均值，回收率為測(cè)定值與理論值的比率。
| 樣本 | 回收率范圍(%) | 平均回收率(%) |
| serum(n=5) | 79-101 | 89 |
| EDTA plasma(n=5) | 79-99 | 85 |
| heparin plasma(n=5) | 87-98 | 91 |
精密度
精密度用樣品測(cè)定值的變異系數(shù)CV表示。CV(%) = SD/mean×100
批內(nèi)差：取同批次試劑盒對(duì)低、中、高值定值樣本進(jìn)行定量檢測(cè),每份樣本連續(xù)測(cè)定20 次，分別計(jì)算不同濃度樣本的平均值及SD值。
批間差：選取3個(gè)不同批次的試劑盒分別對(duì)低、中、高值定值樣本進(jìn)行定量測(cè)定，每個(gè)樣本使用同一試劑盒重復(fù)測(cè)定8次，分別計(jì)算不同濃度樣本的平均值及SD值。
批內(nèi)差： CV<10%
批間差： CV<12%
線性
在定值血清及血漿樣本內(nèi)加入適量的促腎上腺皮質(zhì)激素(ACTH)，并倍比稀釋成1:2，1:4，1:8，1:16的待測(cè)樣本，線性范圍即為稀釋后樣本中促腎上腺皮質(zhì)激素(ACTH)含量的測(cè)定值與理論值的比率。
| 樣本 | 1:2 | 1:4 | 1:8 | 1:16 |
| serum(n=5) | 99-105% | 97-104% | 93-101% | 81-90% |
| EDTA plasma(n=5) | 78-98% | 83-97% | 81-101% | 89-96% |
| heparin plasma(n=5) | 78-98% | 91-105% | 81-96% | 95-102% |
穩(wěn)定性
經(jīng)測(cè)定，試劑盒在有效期內(nèi)按推薦溫度保存，其活性降低率小于5%。
為減小外部因素對(duì)試劑盒破壞前后檢測(cè)值的影響，實(shí)驗(yàn)室的環(huán)境條件需盡量保持一致，尤其是實(shí)驗(yàn)室內(nèi)溫度、濕度及溫育條件。其次由同一實(shí)驗(yàn)員來進(jìn)行操作可減少人為誤差。
實(shí)驗(yàn)流程
1. 實(shí)驗(yàn)前標(biāo)準(zhǔn)品、試劑及樣本的準(zhǔn)備；
2. 加樣（標(biāo)準(zhǔn)品及樣本）50µL，
加入50µL檢測(cè)液A(臨用前配制);
37°C溫育1小時(shí)。
3. 洗板3次；
4. 加檢測(cè)溶液B100µL，37°C孵育30分鐘；
5. 洗板5次；
6. 加底物100µL，37°C孵育10分鐘；
7. 讀數(shù)。
實(shí)驗(yàn)原理
本試劑盒應(yīng)用競(jìng)爭(zhēng)抑制酶聯(lián)免疫分析法測(cè)定標(biāo)本中待測(cè)物質(zhì)水平。將促腎上腺皮質(zhì)激素(ACTH)單克隆抗體包被微孔板，制成固相載體，往包被抗體的微孔中同時(shí)加入生物素標(biāo)記的抗原和待測(cè)抗原（標(biāo)準(zhǔn)品或樣本），待測(cè)抗原與生物素標(biāo)記抗原對(duì)特異性抗體進(jìn)行競(jìng)爭(zhēng)結(jié)合。溫育后經(jīng)洗滌去掉未結(jié)合物，然后加入HRP標(biāo)記的親和素，再次徹底洗滌后加入底物。加入底物后會(huì)產(chǎn)生輝光型光信號(hào)。發(fā)射光強(qiáng)度和樣品中的促腎上腺皮質(zhì)激素(ACTH)呈負(fù)相關(guān)。用化學(xué)發(fā)光儀測(cè)定相對(duì)光單位（RLU），計(jì)算樣品濃度。
贈(zèng)品
相關(guān)產(chǎn)品
| 編號(hào) | 適用物種：Homo sapiens (Human，人) | 應(yīng)用(僅供研究使用，不用于臨床診斷！) |
| UCPA836Hu11 | 促腎上腺皮質(zhì)激素(ACTH)牛血清白蛋白偶聯(lián)物 | Immunogen; SDS-PAGE; WB. |
| UCPA836Hu21 | 促腎上腺皮質(zhì)激素(ACTH)卵白蛋白偶聯(lián)物 | Immunogen; SDS-PAGE; WB. |
| UPAA836Hu08 | 促腎上腺皮質(zhì)激素(ACTH)多克隆抗體 | ELISA, CLIA. / IHC-Fr, ICC, IP (predicted). |
| UPAA836Hu02 | 促腎上腺皮質(zhì)激素(ACTH)多克隆抗體 | ELISA, CLIA. / IHC-Fr, ICC, IP (predicted). |
| ULAA836Hu81 | 促腎上腺皮質(zhì)激素(ACTH)多克隆抗體(異硫氰酸熒光素標(biāo)記) | WB; IHC; ICC; IF. |
| UMAA836Hu21 | 促腎上腺皮質(zhì)激素(ACTH)單克隆抗體 | WB |
| UMAA836Hu28 | 促腎上腺皮質(zhì)激素(ACTH)單克隆抗體 | ELISA, CLIA. / IHC-Fr, ICC, IP (predicted). |
| UCEA836Hu | 促腎上腺皮質(zhì)激素(ACTH)檢測(cè)試劑盒(酶聯(lián)免疫吸附試驗(yàn)法) | Enzyme-linked immunosorbent assay for Antigen Detection. |
| UCCA836Hu | 促腎上腺皮質(zhì)激素(ACTH)檢測(cè)試劑盒(化學(xué)發(fā)光免疫分析法) | Chemiluminescent immunoassay for Antigen Detection. |
| ULMA836Hu | 促腎上腺皮質(zhì)激素(ACTH)等多因子檢測(cè)試劑盒(流式熒光發(fā)光法) | FLIA Kit for Antigen Detection. |
| UKSA836Hu11 | 促腎上腺皮質(zhì)激素(ACTH)檢測(cè)試劑盒DIY材料(酶聯(lián)免疫吸附試驗(yàn)法) | Main materials for "Do It (ELISA Kit) Yourself". |
參考文獻(xiàn)
| 雜志 | 參考文獻(xiàn) |
| Respir Res. | Intracerebroventricular injection of leukotriene B4 attenuates antigen-induced asthmatic response via BLT1 receptor stimulating HPA-axis in sensitized rats. [PubMed: 20403205] |
| Toxicology Letters | Regulation of corticosterone secretion is modified by PFOS exposure at different levels of the hypothalamic–pituitary–adrenal axis in adult male rats [Pubmed:24440345] |
| Mol Med Rep. | Ursolic acid suppresses growth and adrenocorticotrophic hormone secretion in AtT20 cells as a potential agent targeting adrenocorticotrophic hormone-producing pituitary adenoma. [Pubmed:24682498] |
| Peptides | Differential stress response in rats subjected to chronic mild stress is accompanied by changes in CRH-family gene expression at the pituitary level [Pubmed:25236411] |
| Journal of Animal and Feed Sciences | Interdependence of the peripheral metabolism of glucocorticoids and thyroid hormones under calorie deficit in rats at different ages [Researchgate:Source] |
| International Journal of Immunopathology & Pharmacology | Sea buckthorn (Hippophae rhamnoides L.) oil protects against chronic stress-induced inhibitory function of natural killer cells in rats [PubMed: 26684638] |
| Cell Tissue Res | CXCL10/CXCR3 signaling mediates inhibitory action by interferon-gamma on CRF-stimulated adrenocorticotropic hormone (ACTH) release [PubMed: 26572542] |
| Neuroscience Letters | Electroacupuncture reCavia (Guinea pig )late hypothalamic–pituitary–adrenal axis and enhance hippocampal serotonin system in a rat model of depression [Pubmed:26773866] |
| Neural Regeneration Research | Moxibustion?upreCavia (Guinea pig )lates?hippocampal?progranulin?expression. [Pubmed:27212922] |
| Environmental Science and Pollution Research | Lycopene ameliorates atrazine-induced oxidative damage in adrenal cortex of male rats by activation of the Nrf2/HO-1 pathway [Pubmed:27102619] |
| Sci Rep. | Hedgehog signaling activation induces stem cell proliferation and hormone release in the adult pituitary gland [Pubmed:27109116] |
| Sci Rep. | High activity of the stress promoter contributes to susceptibility to stress in the tree shrew [Pubmed:27125313] |
| brain research bulletin | Activation of ATP-sensitive potassium channel by iptakalim normalizes stress-induced HPA axis disorder and depressive behaviour by alleviating inflammation and oxidative stress in mouse hypothalamus. [pubmed:28161195] |
| 中國(guó)臨床藥理學(xué)與治療學(xué) | 肉蔻五味丸抗抑郁作用及機(jī)制研究 [9787] |
| Neuroscience | Heat stress-induced neuroinflammation and aberration in monoamine levels in hypothalamus are associated with temperature dysregulation [pubmed:28663093] |
| Toxicology | Mixture effects of azole fungicides on the adrenal gland in a broad dose range [pubmed:28454766] |
| Biomedicine & Pharmacotherapy | Triptolide suppresses growth and hormone secretion in murine pituitary corticotroph tumor cells via NF-kappaB signaling pathway [pubmed:28892788] |
| Hormones and?Behavior | Acth-induced model of depression resistant to tricyclic antidepressants: Neuroendocrine and behavioral changes and influence of long-term magnesium?… [Pubmed:30025718] |
| Frontiers in Pharmacology | Vaccinium bracteatum Leaf Extract Reverses Chronic Restraint Stress-Induced Depression-Like Behavior in Mice: Regulation of Hypothalamic-Pituitary?… [Pubmed:30038568] |
| Molecular and Cellular Endocrinology | Metformin suppresses growth and adrenocorticotrophic hormone secretion in mouse pituitary corticotroph tumor AtT20 cells [10.1016:j.mce.2018.07.007] |
| Frontiers in?Behavioral?Neuroscience | Chronic hippocampal abnormalities and blunted HPA axis in an animal model of repeated unpredictable stress [Pubmed:30079015] |
| Placenta | Human placentophagy: Effects of dehydration and steaming on hormones, metals and bacteria in placental tissue [Pubmed:29941176] |
| BMC?Veterinary?Research | Hypothalamus-pituitary-adrenal axis involves in anti-viral ability through regulation of immune response in piglets infected by highly pathogenic porcine?… [Pubmed:29540178] |
| European Journal of Applied Physiology | Comparison between men and women of volume regulating hormones and aquaporin-2 excretion following graded central hypovolemia [Pubmed: 30564880] |
| Cyber Leninka | ТЭС-ТЕРАПИЯ КАК МЕТОД ПРЕДУПРЕЖДЕНИЯ ДЕЗАДАПТАЦИИ У САМЦОВ КРЫС С ВЫСОКОЙ СТРЕССОУСТОЙЧИВОСТЬЮ [] |
| Serbian Journal of Experimental and Clinical Research | Assessment of the TDCS Influence on Stress-Induced Disorders in Rats with Low Stress Sustainability and Endurance [] |
| Scientific Reports | Mice lacking Casp1, Ifngr and Nos2 genes exhibit altered depressive-and anxiety-like behaviour, and gut microbiome composition [Pubmed: 31015500] |
| alcoholism-clinical and experimental research | Persistent changes in stress‐regulatory genes in pregnant woman or a child with prenatal alcohol exposure [Pubmed: 31329297] |
| Hormones and Behavior | Hypothalamus-pituitary-adrenal axis imbalance and inflammation contribute to sex differences in separation-and restraint-induced depression [Pubmed: 32165183] |
| Prog Neuropsychopharmacol Biol Psychiatry | Progress in Neuropsychopharmacology & Biological Psychiatry [Pubmed: 17134810] |
| Evid Based Complement Alternat Med | So-Ochim-Tang-Gamibang, a Traditional Herbal Formula, Ameliorates Depression by Regulating Hyperactive Glucocorticoid Signaling In Vitro and In Vivo [Pubmed: 33224257] |
| PROGRESS IN NEURO-PSYCHOPHARMACOLOGY & BIOLOGICAL PSYCHIATRY | Repeated unpredictable stress and social isolation induce chronic HPA axis dysfunction and persistent abnormal fear memory [Pubmed: 32682873] |
| Aging Cell | Altered glucocorticoid metabolism represents a feature of macroph‐aging [Pubmed: 32463582] |
| neurochemical research | Anxiolytic and Anti?depressive Like Effects of Translocator Protein (18 kDa) Ligand YL?IPA08 in a Rat Model of Postpartum Depression [Pubmed: 32383026] |
| Behavioral Ecology and Sociobiology? | Exposure to eagle owl feces induces anti-predator behavior, physiology, and hypothalamic gene responses in a subterranean rodent, the plateau zokor (Eospalax?… [] |
| EUROPEAN CHILD & ADOLESCENT PSYCHIATRY | Increased serum brain-derived neurotrophic factor and adrenocorticotropic hormone levels are associated with obsessive compulsive disorder in medication?free??- [33389158] |
| International Journal of?Nanomedicine | Water-Soluble Carbon Dots in Cigarette Mainstream Smoke: Their Properties and the Behavioural, Neuroendocrinological, and Neurotransmitter Changes They ?- [33762821] |
| J Am Heart Assoc | Post?\Cardiac Arrest Hydrocortisone Use Ameliorates Cardiac Mitochondrial Injury in a Male Rat Model of Ventricular Fibrillation Cardiac Arrest [33960200] |
| Effects of Propofol versus Sevoflurane on Postoperative Pain and Neuroendocrine Stress Response in Oocyte Pickup Patients [33936350] |
