環(huán)磷酸腺苷(cAMP)檢測(cè)試劑盒DIY材料(酶聯(lián)免疫吸附試驗(yàn)法)
ELISA Kit DIY Materials for Cyclic Adenosine Monophosphate (cAMP)
c-AMP; 3'-5'-Cyclic Adenosine Monophosphate; Adenosine Cyclophosphate
- 編號(hào)UKSA003Ge11
- 物種Pan-species (General，通用) 相同的名稱，不同的物種。
- 試劑內(nèi)容 包被抗體、生物素標(biāo)記的競(jìng)爭(zhēng)物、標(biāo)準(zhǔn)品、HRP標(biāo)記的鏈霉親和素、TMB底物、96孔板
- 可檢測(cè)樣本serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
- 可適用原理競(jìng)爭(zhēng)抑制酶聯(lián)免疫吸附試驗(yàn)法測(cè)抗原
- 可檢測(cè)范圍246.9-20000pg/mL
- 可適靈敏度92.5pg/mL
- 應(yīng)用Main materials for "Do It (ELISA Kit) Yourself".
- 下載 英文說(shuō)明書 中文說(shuō)明書
- 規(guī)格 96T*596T*10 96T*20 96T*50 96T*100
- 價(jià)格 ¥ 4298 ¥ 7164 ¥ 12895 ¥ 25074 ¥ 42984
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產(chǎn)品包裝(模擬)
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產(chǎn)品包裝(模擬)
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通過(guò)ISO 9001、ISO 13485質(zhì)量體系認(rèn)證
特異性
試劑盒中的抗體對(duì)環(huán)磷酸腺苷(cAMP)具有高靈敏度和高特異性，經(jīng)檢測(cè)與其它相似物質(zhì)無(wú)明顯交叉反應(yīng)。
用法
1.用100μL每孔的工作濃度的包被抗體包被96孔板，4度孵育過(guò)夜或者37度孵育2小時(shí)；
2.洗板1次；
3.用200μL每孔的工作濃度的封閉液,37度孵育1.5小時(shí)；
4.洗板1次。酶標(biāo)板即可用于ELISA實(shí)驗(yàn)，后續(xù)使用方法與ELISA同。
儲(chǔ)存
抗體，標(biāo)準(zhǔn)品和HRP標(biāo)記的鏈霉親和素在-20度保存，TMB需在4度保存，96孔板在室溫保存。所有組分保質(zhì)期為1年，試劑一旦開封，在4度保存時(shí)間為1個(gè)月。
配套試劑
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| 編號(hào) | 適用物種：Pan-species (General，通用) | 應(yīng)用(僅供研究使用，不用于臨床診斷！) |
| UCPA003Ge11 | 環(huán)磷酸腺苷(cAMP)牛血清白蛋白偶聯(lián)物 | Immunogen; SDS-PAGE; WB. |
| UCPA003Ge21 | 環(huán)磷酸腺苷(cAMP)卵白蛋白偶聯(lián)物 | Immunogen; SDS-PAGE; WB. |
| UCPA003Ge41 | 環(huán)磷酸腺苷(cAMP)人血清白蛋白偶聯(lián)物 | Immunogen; SDS-PAGE; WB. |
| UPAA003Ge01 | 環(huán)磷酸腺苷(cAMP)多克隆抗體 | ELISA, CLIA. / IHC-Fr, ICC, IP (predicted). |
| ULAA003Ge71 | 環(huán)磷酸腺苷(cAMP)多克隆抗體(生物素標(biāo)記) | WB; IHC; ICC. |
| ULAA003Ge81 | 環(huán)磷酸腺苷(cAMP)多克隆抗體(異硫氰酸熒光素標(biāo)記) | WB; IHC; ICC; IF. |
| UFAA003Ge81 | 抗環(huán)磷酸腺苷(cAMP)多克隆抗體(異硫氰酸熒光素標(biāo)記) | FCM |
| UMAA003Ge21 | 環(huán)磷酸腺苷(cAMP)單克隆抗體 | ELISA |
| UMAA003Ge01 | 環(huán)磷酸腺苷(cAMP)單克隆抗體 | ELISA, CLIA. / IHC-Fr, ICC, IP (predicted). |
| UMAA003Ge22 | 環(huán)磷酸腺苷(cAMP)單克隆抗體 | ELISA |
| UMAA003Ge23 | 環(huán)磷酸腺苷(cAMP)單克隆抗體 | ELISA |
| UCEA003Ge | 環(huán)磷酸腺苷(cAMP)檢測(cè)試劑盒(酶聯(lián)免疫吸附試驗(yàn)法) | Enzyme-linked immunosorbent assay for Antigen Detection. |
| UMEA003Ge | 環(huán)磷酸腺苷(cAMP)檢測(cè)試劑盒(酶聯(lián)免疫吸附試驗(yàn)法,小樣本) | Enzyme-linked immunosorbent assay for Antigen Detection. |
| ULMA003Ge | 環(huán)磷酸腺苷(cAMP)等多因子檢測(cè)試劑盒(流式熒光發(fā)光法) | FLIA Kit for Antigen Detection. |
| UKSA003Ge11 | 環(huán)磷酸腺苷(cAMP)檢測(cè)試劑盒DIY材料(酶聯(lián)免疫吸附試驗(yàn)法) | Main materials for "Do It (ELISA Kit) Yourself". |
參考文獻(xiàn)
| 雜志 | 參考文獻(xiàn) |
| Int J Clin Exp Pathol | Cold exposure stimulates lipid metabolism, induces inflammatory response in the adipose tissue of mice and promotes the osteogenic differentiation of BMMSCs via the p38 MAPK pathway [PubMed: 26617802] |
| Experimental Dermatology | Light Emitting Diodes Down‐reCavia (Guinea pig )lates Cathelicidin, Kallikrein, and Toll‐like Receptor 2 Expressions in Keratinocytes and Rosacea‐like Mouse Skin [Pubmed:27315464] |
| arteriosclerosis thrombosis and vascular biology | P2Y12 Promotes Migration of Vascular Smooth Muscle Cells Through Cofilin Dephosphorylation During Atherogenesis. [pubmed:28062501] |
| Taylor & Francis | Influences of Hyriopsis cumingii polysaccharides on mice immunosignaling molecules and T lymphocyte differentiation [09540105.2017.1306494] |
| international journal of biological macromolecules | Immunomodulatory effect of APS and PSP is mediated by Ca2+-cAMP and TLR4/NF-κB signaling pathway in macrophage. [pubmed:27732877] |
| Front Pharmacol.? | Ginsenoside Rg5 Inhibits Succinate-Associated Lipolysis in Adipose Tissue and Prevents Muscle Insulin Resistance. [pubmed:28261091] |
| Allergologia et immunopathologia | The relation of innate and adaptive immunity with viral-induced acute asthma attacks: Focusing on IP-10 and cathelicidin [pubmed:27955890] |
| Journal of Enzyme Inhibition and Medicinal Chemistry | Structural re-positioning, molecular modelling, oxidative degradation, and biological screening of linagliptin as adenosine 3 receptor (ADORA3) modulators?… [Pubmed:29768061] |
| Frontiers in Physiology | Astragaloside IV Inhibits Adipose Lipolysis and Reduces Hepatic Glucose Production via Akt Dependent PDE3B Expression in HFD-Fed Mice [Pubmed:29410630] |
| Nanoscience and Nanotechnology Letters | Dobutamine Up-Regulates Aquaporin 5 Expression in Septic Pulmonary Edema Model In Vitro via cAMP-PKA/CREB Signaling Pathway [10.1166:nnl.2018.2602] |
| PLoS One | Tuberculous meningitis is associated with higher cerebrospinal HIV-1 viral loads compared to other HIV-1-associated meningitides [Pubmed:29394269] |
| Theriogenology | Developmental competence of buffalo (Bubalus bubalis) denuded oocytes cocultured with cumulus cells: Protective role of cumulus cells [Pubmed:30092373] |
| Evidence-Based Complementary and Alternative Medicine | iTRAQ-Based Proteomics to Reveal the Mechanism of Hypothalamus in Kidney-Yin Deficiency Rats Induced by Levothyroxine [] |
| EXPERIMENTAL BIOLOGY AND MEDICINE | Calcimimetic R568 improved cardiac remodeling by classic and novel renin-angiotensin system in spontaneously hypertensive rats [Pubmed: 31159562] |
| Scientific Reports | Deletion of the serine protease CAP2/Tmprss4 leads to dysregulated renal water handling upon dietary potassium depletion [Pubmed: 31863073] |
| REPRODUCTION FERTILITY AND DEVELOPMENT | Cytoplasm lipids can be modulated through hormone-sensitive lipase and are related to mitochondrial function in porcine IVM oocytes [Pubmed: 32172783] |
| CANADIAN JOURNAL OF PHYSIOLOGY AND PHARMACOLOGY | Astragaloside IV relieves gestational diabetes mellitus in genetic mice through reducing hepatic gluconeogenesis [Pubmed: 32160476] |
| J Physiol Biochem | Altered absorptive function in the gall bladder during cholesterol gallstone formation is associated with abnormal NHE3 complex formation [Pubmed: 32557227] |
| Biomed Pharmacother | Antihypertensive effects of allicin on spontaneously hypertensive rats via vasorelaxation and hydrogen sulfide mechanisms [Pubmed: 32480217] |
| Legal Medicine | DNA microarray analysis of hypothermia-exposed murine lungs for identification of forensic biomarkers [Pubmed: 33161360] |
| Neuropeptides | Adrenomedullin 2 attenuates LPS-induced inflammation in microglia cells by receptor-mediated cAMP-PKA pathway [33253929] |
| Oxidative Medicine and Cellular Longevity | A Green and Blue Monochromatic Light Combination Therapy Reduces Oxidative Stress and Enhances B-Lymphocyte Proliferation through Promoting ?- [33828639] |
| Physiological crosstalk between the Mel1a and Mel1c pathways modulates melatonin-mediated, monochromatic light combination-induced bursa B-lymphocyte??- [] | |
| Pflügers Archiv : European journal of physiology | The role of adrenergic and muscarinic receptors in stress-induced cardiac injury [34245378] |
| Theranostics | Sult2b1 deficiency exacerbates ischemic stroke by promoting pro-inflammatory macrophage polarization in mice [34815805] |
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