高遷移率族蛋白1(HMGB1)檢測試劑盒DIY材料(酶聯(lián)免疫吸附試驗法)
ELISA Kit DIY Materials for High Mobility Group Protein 1 (HMGB1)
HMG1; HMG3; SBP1; Sulfoglucuronyl Carbohydrate Binding Protein; Amphoterin; High Mobility Group Box 1 Protein
- 編號UKSA399Ra01
- 物種Rattus norvegicus (Rat，大鼠) 相同的名稱，不同的物種。
- 試劑內(nèi)容 包被抗體、檢測抗體、標準品、HRP標記的鏈霉親和素、TMB底物、96孔板
- 可檢測樣本Serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
- 可適用原理雙抗夾心酶聯(lián)免疫吸附試驗法測抗原
- 可檢測范圍31.2-2,000pg/mL
- 可適靈敏度12.5pg/mL
- 應用自己動手制作ELISA試劑盒的主要材料
- 下載 英文說明書 中文說明書
- 規(guī)格 96T*596T*10 96T*20 96T*50 96T*100
- 價格 ¥ 3694 ¥ 6156 ¥ 11081 ¥ 21546 ¥ 36936
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產(chǎn)品包裝(模擬)
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產(chǎn)品包裝(模擬)
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通過ISO 9001、ISO 13485質(zhì)量體系認證
特異性
試劑盒中的抗體對高遷移率族蛋白1(HMG1)具有高靈敏度和高特異性，經(jīng)檢測與其它相似物質(zhì)無明顯交叉反應。
用法
1.用100μL每孔的工作濃度的包被抗體包被96孔板，4度孵育過夜或者37度孵育2小時；
2.洗板1次；
3.用200μL每孔的工作濃度的封閉液,37度孵育1.5小時；
4.洗板1次。酶標板即可用于ELISA實驗，后續(xù)使用方法與ELISA同。
儲存
抗體，標準品和HRP標記的鏈霉親和素在-20度保存，TMB需在4度保存，96孔板在室溫保存。所有組分保質(zhì)期為1年，試劑一旦開封，在4度保存時間為1個月。
配套試劑
增值服務
相關產(chǎn)品
| 編號 | 適用物種：Rattus norvegicus (Rat，大鼠) | 應用(僅供研究使用，不用于臨床診斷！) |
| UAPA399Ra01 | 高遷移率族蛋白1(HMGB1)活性蛋白 | Cell?culture;?Activity?Assays. |
| URPA399Ra01 | 高遷移率族蛋白1(HMGB1)重組蛋白 | Positive Control; Immunogen; SDS-PAGE; WB. |
| UPAA399Ra01 | 高遷移率族蛋白1(HMGB1)多克隆抗體 | WB; IHC |
| ULAA399Ra81 | 高遷移率族蛋白1(HMGB1)多克隆抗體(異硫氰酸熒光素標記) | WB; IHC; ICC; IF. |
| ULAA399Ra71 | 高遷移率族蛋白1(HMGB1)多克隆抗體(生物素標記) | WB |
| UMAA399Ra21 | 高遷移率族蛋白1(HMGB1)單克隆抗體 | WB; IHC; ICC; IP. |
| USEA399Ra | 高遷移率族蛋白1(HMGB1)檢測試劑盒(酶聯(lián)免疫吸附試驗法) | Enzyme-linked immunosorbent assay for Antigen Detection. |
| ULMA399Ra | 高遷移率族蛋白1(HMGB1)等多因子檢測試劑盒(流式熒光發(fā)光法) | FLIA Kit for Antigen Detection. |
| UKSA399Ra01 | 高遷移率族蛋白1(HMGB1)檢測試劑盒DIY材料(酶聯(lián)免疫吸附試驗法) | 自己動手制作ELISA試劑盒的主要材料 |
參考文獻
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| Sci Rep | Intracellular translocation of HMGB1 is important for Zika virus replication in Huh7 cells [Pubmed:35058496] |
| ORL J Otorhinolaryngol Relat Spec | High Mobility Group Box-1 Protein and Interleukin 33 Expression in Allergic Rhinitis [Pubmed:34979505] |
| J Exp Clin Cancer Res | SKP2 drives the sensitivity to neddylation inhibitors and cisplatin in malignant pleural mesothelioma [Pubmed:35197103] |
| Am J Physiol Gastrointest Liver Physiol | Abnormal intestinal milieu in post-traumatic stress disorder is not impacted by treatment that improves symptoms [Pubmed:35638693] |
| Journal of the South African Veterinary Association | Circulating markers of endothelial activation in canine parvoviral enteritis [] |
| Reseach square | The antiviral action of resveratrol against Zika virus through the regulation of interferon responses by HMGB1 [] |
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