白介素13(IL13)檢測試劑盒DIY材料(酶聯(lián)免疫吸附試驗(yàn)法)
ELISA Kit DIY Materials for Interleukin 13 (IL13)
ALRH; BHR1; P600; IL-13; NC30
- 編號UKSA060Hu01
- 物種Homo sapiens (Human，人) 相同的名稱，不同的物種。
- 試劑內(nèi)容 包被抗體、檢測抗體、標(biāo)準(zhǔn)品、HRP標(biāo)記的鏈霉親和素、TMB底物、96孔板
- 可檢測樣本Serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids.
- 可適用原理雙抗夾心酶聯(lián)免疫吸附試驗(yàn)法測抗原
- 可檢測范圍15.6-1,000pg/mL
- 可適靈敏度6.7pg/mL
- 應(yīng)用自己動(dòng)手制作ELISA試劑盒的主要材料
- 下載 英文說明書 中文說明書
- 規(guī)格 96T*596T*10 96T*20 96T*50 96T*100
- 價(jià)格 ¥ 3402 ¥ 5670 ¥ 10206 ¥ 19845 ¥ 34020
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產(chǎn)品包裝(模擬)
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產(chǎn)品包裝(模擬)
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通過ISO 9001、ISO 13485質(zhì)量體系認(rèn)證
特異性
試劑盒中的抗體對白介素13(IL13)具有高靈敏度和高特異性，經(jīng)檢測與其它相似物質(zhì)無明顯交叉反應(yīng)。
用法
1.用100μL每孔的工作濃度的包被抗體包被96孔板，4度孵育過夜或者37度孵育2小時(shí)；
2.洗板1次；
3.用200μL每孔的工作濃度的封閉液,37度孵育1.5小時(shí)；
4.洗板1次。酶標(biāo)板即可用于ELISA實(shí)驗(yàn)，后續(xù)使用方法與ELISA同。
儲(chǔ)存
抗體，標(biāo)準(zhǔn)品和HRP標(biāo)記的鏈霉親和素在-20度保存，TMB需在4度保存，96孔板在室溫保存。所有組分保質(zhì)期為1年，試劑一旦開封，在4度保存時(shí)間為1個(gè)月。
配套試劑
贈(zèng)品
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相關(guān)產(chǎn)品
| 編號 | 適用物種：Homo sapiens (Human，人) | 應(yīng)用(僅供研究使用，不用于臨床診斷！) |
| UAPA060Hu01 | 白介素13(IL13)活性蛋白 | Cell?culture;?Activity?Assays. |
| UEPA060Hu61 | 白介素13(IL13)真核蛋白 | Positive Control; Immunogen; SDS-PAGE; WB. |
| URPA060Hu01 | 白介素13(IL13)重組蛋白 | Positive Control; Immunogen; SDS-PAGE; WB. |
| UAPA060Hu62 | 白介素13(IL13)活性蛋白 | Cell?culture;?Activity?Assays. |
| UEPA060Hu62 | 白介素13(IL13)真核蛋白 | Positive Control; Immunogen; SDS-PAGE; WB. |
| UPAA060Hu01 | 白介素13(IL13)多克隆抗體 | IHC |
| URAA060Hu21 | 白介素13(IL13)重組抗體 | WB; IF; ICC; IHC; IP; FCM. |
| UMAA060Hu22 | 白介素13(IL13)單克隆抗體 | WB; IHC; ICC; IP. |
| USEA060Hu | 白介素13(IL13)檢測試劑盒(酶聯(lián)免疫吸附試驗(yàn)法) | Enzyme-linked immunosorbent assay for Antigen Detection. |
| ULMA060Hu | 白介素13(IL13)等多因子檢測試劑盒(流式熒光發(fā)光法) | FLIA Kit for Antigen Detection. |
| UKSA060Hu01 | 白介素13(IL13)檢測試劑盒DIY材料(酶聯(lián)免疫吸附試驗(yàn)法) | 自己動(dòng)手制作ELISA試劑盒的主要材料 |
參考文獻(xiàn)
| 雜志 | 參考文獻(xiàn) |
| Critical Care Medicine | Systemic release of cytokines and heat shock proteins in porcine models of polytrauma and hemorrhage [PubMed: PMC3383056] |
| Int J Clin Exp Pathol | Downregulation of SUMF2 gene in ovalbumin-induced rat model of allergic inflammation [PubMed: 26722390] |
| Advances in Experimental Medicine and Biology? | Antiinflammatory Effect of N-Acetylcysteine Combined with Exogenous Surfactant in Meconium-Induced Lung Injury [Pubmed:27283193] |
| Molecular Immunology | Targeted inhibition of GATA-6 attenuates airway inflammation and remodeling by reCavia (Guinea pig )lating caveolin-1 through TLR2/MyD88/NF-??B in murine model of asthma [Pubmed:27289030] |
| Journal of Agricultural Biotechnology | Gln Attenuates LPS-induced Inflammatory Injury of Bos taurus Testis Sustentacular Cells [10610] |
| BMC Veterinary Research | Lymphocytic, cytokine and transcriptomic profiles in peripheral blood of dogs with atopic dermatitis [pubmed:27553600] |
| Molecular?Immunology | Targeted inhibition of GATA-6 attenuates airway inflammation and remodeling by regulating caveolin-1 through TLR2/MyD88/NF-κB in murine model of asthma [pubmed:27289030] |
| Physiological?Research | Lung inflammatory and oxidative alterations after exogenous surfactant therapy fortified with budesonide in rabbit model of meconium aspiration syndrome. [pubmed:28006947] |
| Gut Pathog. | Oral administration of Clostridium butyricum CGMCC0313‐1 reduces ovalbumin‐induced allergic airway inflammation in mice [pubmed:28250847] |
| Veterinary Immunology and Immunopathology | Cytokine levels in colostrum and in foals' serum pre- and post-suckling [pubmed:28242000] |
| Asian Pacific Society of Respirology | Oral administration of Clostridium butyricum CGMCC0313-1 reduces ovalbumin-induced allergic airway inflammation in mice [pubmed:28122397] |
| Journal of Autoimmunity | Commensal bacteria aggravate allergic asthma via NLRP3/IL-1β signaling in post-weaning mice [10.1016:j.jaut.2018.07.003] |
| bulletin of experimental biology and medicine | Quantitative and Qualitative Characterization of Phagocytic Activity of Macrophages of Bone Marrow and Fetal Origin [Pubmed: 31183654] |
| life sciences | Melatonin biosynthesis restored by CpG oligodeoxynucleotides attenuates allergic airway inflammation via regulating NLRP3 inflammasome [Pubmed: 31738882] |
| Frontiers in Immunology | TLR2-Melatonin Feedback Loop Regulates the Activation of NLRP3 Inflammasome in Murine Allergic Airway Inflammation [Pubmed: 32117301] |
| Parasites & Vectors | Fasciola gigantica excretory-secretory products (FgESPs) modulate the differentiation and immune functions of buffalo dendritic cells through a mechanism?… [Pubmed: 32680553] |
| Biomedicine & Pharmacotherapy | Qingfei oral liquid alleviates airway hyperresponsiveness and mucus hypersecretion via TRPV1 signaling in RSV-infected asthmatic mice [Pubmed: 32521453] |
| Journal of Traditional Chinese Medical Sciences | Mahuang Xixin Fuzi Decoction protects against ovalbumin-induced allergic rhinitis by inhibiting type 2 innate lymphoid cells in mice [] |
| Traditional Medicine Research | Qingfei oral liquid downregulates TRPV1 expression to reduce airway inflammation and mucus hypersecretion injury caused by respiratory syncytial virus infection … [] |
| Applied materials today | Tanshinone IIA-loaded aligned microfibers facilitate stem cell recruitment and capillary formation by inducing M2 macrophage polarization [] |
| Aggravation of Airway Inflammation in RSV-Infected Asthmatic Mice Following Infection-Induced Alteration of Gut Microbiota [] | |
| Biomed Pharmacother | Qingfei oral liquid inhibited autophagy to alleviate inflammation via mTOR signaling pathway in RSV-infected asthmatic mice [33706133] |
| BIOMATERIALS | Aligned microfiber-induced macrophage polarization to guide schwann-cell-enabled peripheral nerve regeneration [33813259] |
| PLoS One | Beneficial impact of cathelicidin on hypersensitivity pneumonitis treatment?aIn vivo studies [33999928] |
| Anti-inflammatory activity of non-selective PDE inhibitor aminophylline on the lung tissue and respiratory parameters in animal model of ARDS [] |
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