激活素A(ACVA)檢測試劑盒(酶聯(lián)免疫吸附試驗法)
ELISA Kit for Activin A (ACVA)
ACV-A; Activin Beta A Beta A Homodimer
- 編號USEA001Hu
- 物種Homo sapiens (Human，人) 相同的名稱，不同的物種。
- 實驗方法雙抗夾心
- 反應(yīng)時長3h
- 檢測范圍15.6-1,000pg/mL
- 靈敏度最小可檢測劑量小于等于6.2pg/mL.
- 樣本類型serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
- 下載 英文說明書 中文說明書
- 規(guī)格 48T96T 96T*5 96T*10 96T*100
- 價格 ¥ 1323 ¥ 1890 ¥ 8505 ¥ 16065 ¥ 132300
- 欲了解實際交易價格和更多情況，請與當(dāng)?shù)亟?jīng)銷商聯(lián)系!
-
產(chǎn)品包裝(模擬)
-
產(chǎn)品包裝(模擬)
-
實驗結(jié)果圖
-
標(biāo)準(zhǔn)曲線圖
-
通過ISO 9001、ISO 13485質(zhì)量體系認(rèn)證
特異性
本試劑盒用于檢測激活素A(ACVA)，經(jīng)檢測與其它相似物質(zhì)無明顯交叉反應(yīng)。
由于受到技術(shù)及樣本來源的限制，不可能完成對所有相關(guān)或相似物質(zhì)交叉反應(yīng)檢測，因此本試劑盒有可能與未經(jīng)檢測的其它物質(zhì)有交叉反應(yīng)。
回收率
分別于定值血清及血漿樣本中加入一定量的激活素A(ACVA)（加標(biāo)樣品），重復(fù)測定并計算其均值，回收率為測定值與理論值的比率。
| 樣本 | 回收率范圍(%) | 平均回收率(%) |
| serum(n=5) | 85-92 | 88 |
| EDTA plasma(n=5) | 81-91 | 87 |
| heparin plasma(n=5) | 82-96 | 85 |
精密度
精密度用樣品測定值的變異系數(shù)CV表示。CV(%) = SD/mean×100
批內(nèi)差：取同批次試劑盒對低、中、高值定值樣本進(jìn)行定量檢測,每份樣本連續(xù)測定20 次，分別計算不同濃度樣本的平均值及SD值。
批間差：選取3個不同批次的試劑盒分別對低、中、高值定值樣本進(jìn)行定量測定，每個樣本使用同一試劑盒重復(fù)測定8次，分別計算不同濃度樣本的平均值及SD值。
批內(nèi)差： CV<10%
批間差： CV<12%
線性
在定值血清及血漿樣本內(nèi)加入適量的激活素A(ACVA)，并倍比稀釋成1:2，1:4，1:8，1:16的待測樣本，線性范圍即為稀釋后樣本中激活素A(ACVA)含量的測定值與理論值的比率。
| 樣本 | 1:2 | 1:4 | 1:8 | 1:16 |
| serum(n=5) | 86-97% | 82-89% | 95-102% | 80-99% |
| EDTA plasma(n=5) | 88-97% | 78-98% | 99-105% | 86-96% |
| heparin plasma(n=5) | 96-104% | 90-104% | 88-96% | 93-101% |
穩(wěn)定性
經(jīng)測定，試劑盒在有效期內(nèi)按推薦溫度保存，其活性降低率小于5%。
為減小外部因素對試劑盒破壞前后檢測值的影響，實驗室的環(huán)境條件需盡量保持一致，尤其是實驗室內(nèi)溫度、濕度及溫育條件。其次由同一實驗員來進(jìn)行操作可減少人為誤差。
實驗流程
1. 實驗前標(biāo)準(zhǔn)品、試劑及樣本的準(zhǔn)備；
2. 加樣（標(biāo)準(zhǔn)品及樣本）100µL，37°C孵育1小時；
3. 吸棄，加檢測溶液A100µL，37°C孵育1小時；
4. 洗板3次；
5. 加檢測溶液B100µL，37°C孵育30分鐘；
6. 洗板5次；
7. 加TMB底物90µL，37°C孵育10-20分鐘；
8. 加終止液50µL，立即450nm讀數(shù)。
實驗原理
將激活素A(ACVA)抗體包被于96孔微孔板中，制成固相載體，向微孔中分別加入標(biāo)準(zhǔn)品或標(biāo)本，其中的激活素A(ACVA)與連接于固相載體上的抗體結(jié)合，然后加入生物素化的激活素A(ACVA)抗體，將未結(jié)合的生物素化抗體洗凈后，加入HRP標(biāo)記的親和素，再次徹底洗滌后加入TMB底物顯色。TMB在過氧化物酶的催化下轉(zhuǎn)化成藍(lán)色，并在酸的作用下轉(zhuǎn)化成最終的黃色。顏色的深淺和樣品中的激活素A(ACVA)呈正相關(guān)。用酶標(biāo)儀在450nm波長下測定吸光度(O.D.值)，計算樣品濃度。
增值服務(wù)
相關(guān)產(chǎn)品
| 編號 | 適用物種：Homo sapiens (Human，人) | 應(yīng)用(僅供研究使用，不用于臨床診斷！) |
| URPA001Hu01 | 激活素A(ACVA)重組蛋白 | Positive Control; Immunogen; SDS-PAGE; WB. |
| UAPA001Hu01 | 激活素A(ACVA)活性蛋白 | Cell?culture;?Activity?Assays. |
| UPAA001Hu01 | 激活素A(ACVA)多克隆抗體 | IHC |
| USEA001Hu | 激活素A(ACVA)檢測試劑盒(酶聯(lián)免疫吸附試驗法) | Enzyme-linked immunosorbent assay for Antigen Detection. |
| UCEA001Hu | 激活素A(ACVA)檢測試劑盒(酶聯(lián)免疫吸附試驗法) | Enzyme-linked immunosorbent assay for Antigen Detection. |
| USCA001Hu | 激活素A(ACVA)檢測試劑盒(化學(xué)發(fā)光免疫分析法) | Chemiluminescent immunoassay for Antigen Detection. |
| ULMA001Hu | 激活素A(ACVA)等多因子檢測試劑盒(流式熒光發(fā)光法) | FLIA Kit for Antigen Detection. |
參考文獻(xiàn)
| 雜志 | 參考文獻(xiàn) |
| 13 | Secretory Products From Epicardial Adipose Tissue of Patients With Type 2 Diabetes Mellitus Induce Cardiomyocyte Dysfunction [Ahajournals: Source] |
| J. Pharmacogn | [Article: Hksobf_2015_V46N2_167] |
| United States Patent Application | Methods for Diagnosis and Treatment of Concussion or Brain Injury [2016:0103140] |
| BMC Cancer. | Activins and their related proteins in colon carcinogenesis: insights from early and advanced azoxymethane rat models of colon cancer. [pubmed:27835986] |
| Basic Clin Pharmacol Toxicol. | Magnolol Attenuates Concanavalin A-induced Hepatic Fibrosis, Inhibits CD4+ T Helper 17 (Th17) Cell Differentiation and Suppresses Hepatic Stellate Cell Activation: Blockade of Smad3/Smad4 Signalling. [pubmed:28032440] |
| Basic & Clinical Pharmacology & Toxicology | Magnolol Attenuates Concanavalin A-induced Hepatic Fibrosis, Inhibits CD4 + T Helper 17(Th17) Cell Differentiation and Suppresses Hepatic Stellate Cell Activation: Blockade of Smad3/Smad4 Signalling [doi:10.1111] |
| Journal of Immunology | MicroRNA-27b Modulates Inflammatory Response and Apoptosis during Mycobacterium tuberculosis Infection [Pubmed:29661829] |
| Journal of?Vascular?Surgery | Expression of platelet-derived growth factor B is upregulated in patients with thoracic aortic dissection [Pubmed:29685513] |
| Acta Biochimica et Biophysica Sinica | Toxic effects of arsenic trioxide on protoscoleces through ROS production, and Ca-ER stress-dependent apoptosis [10.1093:abbs] |
| Poultry?Science | The effect of raw and fermented rapeseed cake on the metabolic parameters, immune status, and intestinal morphology of turkeys [Pubmed:29917099] |
| Food Bioscience | Characterization of Phospholipids from Pacific Saury () Viscera and Their Neuroprotective Activity [10.1016:j.fbio.2018.06.002] |
| Current Analytical Chemistry | Single Wall Carbon Nanotube and Magnetic Bead Based Electrochemical Immunosensor for Sensitive Detection of Salivary Secretory Immunoglobulin A [10.2174:2211556006666170525161225] |
| Journal?of?Veterinary?Internal?Medicine | FRIDAY, NOVEMBER 3, 2017 11.30-12.0 BALLROOM AB [:] |
| Universal Journal of Pharmaceutical Research | SEROPREVALENCE OF ANTI-MANNOSE BINDING LECTIN AUTOANTIBODIES IN PATIENTS WITH RHEUMATOID ARTHRITIS IN SANA'A CITY-YEMEN [10.22270:ujpr.v3i2.138] |
| Involvement of RBP4 in diabetic atherosclerosis and the role of vitamin D intervention [:] | |
| Clin Oral Investig.? | Gingival crevicular fluid levels of human beta-defensin-1 in type 2 diabetes mellitus and periodontitis [Pubmed:29713888] |
| Myocardial fibrosis in hypertrophic cardiomyopathy [:] | |
| TELLING TRANSVERSAL COMPETENCES… TO BE PROFESSIONALLY PROMOTED [:] | |
| Protein biomarker panels for detecting colorectal cancer and advanced adenoma [:] | |
| Endothelotropic activity of 4-hydroxy-3, 5-di-tret-butylcinnamic acid in the conditions of experimental cerebral ischemia [:] | |
| Silencing of the CSNK2β gene by siRNA inhibits invasiveness and growth of MDA-MB-231 cells [10.1101:311092] | |
| Duchenne Muscular Dystrophy | Flow Cytometry-Defined CD49d Expression in Circulating T-Lymphocytes Is a Biomarker for Disease Progression in Duchenne Muscular Dystrophy [Pubmed:29067667] |
| S | S [S] |
| P | P [P] |
| D | D [D] |
| h | h [h] |
| O | O [O] |
| M | M [M] |
| E | E [E] |
| I | I [I] |
| B | B [B] |
| T | T [T] |
| N | N [N] |
| [] | |
| [] | |
| L | L [L] |
| J | J [J] |
| Journal of Geriatric?Oncology | Serum biomarkers for predicting overall survival and early mortality in older patients with metastatic solid tumors [Pubmed: 30952517] |
| clinica chimica acta | The performances of serum activins and follistatin in the diagnosis of ectopic pregnancy: A prospective case-control study [Pubmed: 31647905] |
| hindawi | The Association between the Activin A Serum Level and Carotid Intima-Media Thickness in Chronic Kidney Disease Patients [] |
| Pathology & Oncology Research | Profiling Activins and Follistatin in Colorectal Cancer According to Clinical Stage, Tumour Sidedness and Smad4 Status [34867090] |
