鐵調(diào)素(Hepc)檢測試劑盒(酶聯(lián)免疫吸附試驗(yàn)法)
ELISA Kit for Hepcidin (Hepc)
HAMP; HFE2B; PLTR; LEAP1; Hepcidin Antimicrobial Peptide; Liver-expressed antimicrobial peptide 1; Putative liver tumor regressor
- 編號USEB979Ca
- 物種Canis familiaris; Canine (Dog，犬) 相同的名稱，不同的物種。
- 實(shí)驗(yàn)方法雙抗夾心
- 反應(yīng)時長3h
- 檢測范圍0.625-40ng/mL
- 靈敏度最小可檢測劑量小于等于0.244ng/mL.
- 樣本類型Serum, plasma and other biological fluids
- 下載 英文說明書 中文說明書
- 規(guī)格 48T96T 96T*5 96T*10 96T*100
- 價格 ¥ 1596 ¥ 2280 ¥ 10260 ¥ 19380 ¥ 159600
- 欲了解實(shí)際交易價格和更多情況，請與當(dāng)?shù)亟?jīng)銷商聯(lián)系!
-
產(chǎn)品包裝(模擬)
-
產(chǎn)品包裝(模擬)
-
實(shí)驗(yàn)結(jié)果圖
-
標(biāo)準(zhǔn)曲線圖
-
通過ISO 9001、ISO 13485質(zhì)量體系認(rèn)證
特異性
本試劑盒用于檢測鐵調(diào)素(Hepc)，經(jīng)檢測與其它相似物質(zhì)無明顯交叉反應(yīng)。
由于受到技術(shù)及樣本來源的限制，不可能完成對所有相關(guān)或相似物質(zhì)交叉反應(yīng)檢測，因此本試劑盒有可能與未經(jīng)檢測的其它物質(zhì)有交叉反應(yīng)。
回收率
分別于定值血清及血漿樣本中加入一定量的鐵調(diào)素(Hepc)（加標(biāo)樣品），重復(fù)測定并計算其均值，回收率為測定值與理論值的比率。
| 樣本 | 回收率范圍(%) | 平均回收率(%) |
| serum(n=5) | 94-104 | 101 |
| EDTA plasma(n=5) | 95-105 | 101 |
| heparin plasma(n=5) | 82-97 | 91 |
精密度
精密度用樣品測定值的變異系數(shù)CV表示。CV(%) = SD/mean×100
批內(nèi)差：取同批次試劑盒對低、中、高值定值樣本進(jìn)行定量檢測,每份樣本連續(xù)測定20 次，分別計算不同濃度樣本的平均值及SD值。
批間差：選取3個不同批次的試劑盒分別對低、中、高值定值樣本進(jìn)行定量測定，每個樣本使用同一試劑盒重復(fù)測定8次，分別計算不同濃度樣本的平均值及SD值。
批內(nèi)差： CV<10%
批間差： CV<12%
線性
在定值血清及血漿樣本內(nèi)加入適量的鐵調(diào)素(Hepc)，并倍比稀釋成1:2，1:4，1:8，1:16的待測樣本，線性范圍即為稀釋后樣本中鐵調(diào)素(Hepc)含量的測定值與理論值的比率。
| 樣本 | 1:2 | 1:4 | 1:8 | 1:16 |
| serum(n=5) | 96-103% | 78-89% | 94-102% | 83-96% |
| EDTA plasma(n=5) | 87-95% | 82-91% | 89-98% | 83-101% |
| heparin plasma(n=5) | 80-104% | 88-102% | 91-105% | 86-93% |
穩(wěn)定性
經(jīng)測定，試劑盒在有效期內(nèi)按推薦溫度保存，其活性降低率小于5%。
為減小外部因素對試劑盒破壞前后檢測值的影響，實(shí)驗(yàn)室的環(huán)境條件需盡量保持一致，尤其是實(shí)驗(yàn)室內(nèi)溫度、濕度及溫育條件。其次由同一實(shí)驗(yàn)員來進(jìn)行操作可減少人為誤差。
實(shí)驗(yàn)流程
1. 實(shí)驗(yàn)前標(biāo)準(zhǔn)品、試劑及樣本的準(zhǔn)備；
2. 加樣（標(biāo)準(zhǔn)品及樣本）100µL，37°C孵育1小時；
3. 吸棄，加檢測溶液A100µL，37°C孵育1小時；
4. 洗板3次；
5. 加檢測溶液B100µL，37°C孵育30分鐘；
6. 洗板5次；
7. 加TMB底物90µL，37°C孵育10-20分鐘；
8. 加終止液50µL，立即450nm讀數(shù)。
實(shí)驗(yàn)原理
將鐵調(diào)素(Hepc)抗體包被于96孔微孔板中，制成固相載體，向微孔中分別加入標(biāo)準(zhǔn)品或標(biāo)本，其中的鐵調(diào)素(Hepc)與連接于固相載體上的抗體結(jié)合，然后加入生物素化的鐵調(diào)素(Hepc)抗體，將未結(jié)合的生物素化抗體洗凈后，加入HRP標(biāo)記的親和素，再次徹底洗滌后加入TMB底物顯色。TMB在過氧化物酶的催化下轉(zhuǎn)化成藍(lán)色，并在酸的作用下轉(zhuǎn)化成最終的黃色。顏色的深淺和樣品中的鐵調(diào)素(Hepc)呈正相關(guān)。用酶標(biāo)儀在450nm波長下測定吸光度(O.D.值)，計算樣品濃度。
增值服務(wù)
相關(guān)產(chǎn)品
| 編號 | 適用物種：Canis familiaris; Canine (Dog，犬) | 應(yīng)用(僅供研究使用，不用于臨床診斷！) |
| URPB979Ca01 | 鐵調(diào)素(Hepc)重組蛋白 | Positive Control; Immunogen; SDS-PAGE; WB. |
| USEB979Ca | 鐵調(diào)素(Hepc)檢測試劑盒(酶聯(lián)免疫吸附試驗(yàn)法) | Enzyme-linked immunosorbent assay for Antigen Detection. |
| ULMB979Ca | 鐵調(diào)素(Hepc)等多因子檢測試劑盒(流式熒光發(fā)光法) | FLIA Kit for Antigen Detection. |
參考文獻(xiàn)
| 雜志 | 參考文獻(xiàn) |
| World J Gastroenterol. | Hepcidin expression in colon during trinitrobenzene sulfonic acid-induced colitis in rats [Pubmed: 24764672] |
| International Journal of Advanced Research | Modulations of serum hepcidin, IL6 and iron status by different exercise regimens in ovarectomized rats [Com:Source] |
| Neurochemical Journal | Serum iron metabolism markers including hepcidin in multiple sclerosis patients [Springer:Source] |
| Afro-Egypt J Infect Endem Dis | Monitoring Hepcidin Level in Chronic Hepatitis C Virus Patients during Therapy [Zu:Source] |
| Gastroenterology | Gluconeogenic signals regulate iron homeostasis via hepcidin in mice [Pubmed:Pmc3989026] |
| J Card Fail. | Inappropriate expression of hepcidin by liver congestion contributes to anemia and relative iron deficiency [Pubmed:24440572] |
| Food Funct. | The action of JAK, SMAD and ERK signal pathways on hepcidin suppression by polysaccharides from Angelica sinensis in rats with iron deficiency anemia [Pubmed:24752529] |
| Free Radic Res. | Changes in skeletal muscle iron metabolism outpace amyotrophic lateral sclerosis onset in transgenic rats bearing the G93A hmSOD1 gene mutation [Pubmed:25175826] |
| Saudi J Kidney Dis Transpl. | Hepcidin and regulation of iron homeostasis in maintenance hemodialysis patients [Pubmed:25193892] |
| J Endocrinol Invest.? | The interplay among iron metabolism, endothelium and inflammatory cascade in dysmetabolic disorders [Pubmed:25245337] |
| Gastroenterology Research and Practice | An Evaluation of the Correlation between Hepcidin Serum Levels and Disease Activity in Inflammatory Bowel Disease [Pubmed:25628652] |
| Clin Lab. | Serum hepcidin levels in Bulgarian population. [Pubmed:25651734] |
| The Tohoku Journal of Experimental Medicine | Liver Congestion in Heart Failure Contributes to Inappropriately Increased Serum Hepcidin despite Anemia [Pubmed:25742771] |
| Cancer Res | Hepcidin regulation in prostate and its disruption in prostate cancer [PubMed: 25858146] |
| Biochem Biophys Res Commun | B-cell translocation gene 2 promotes hepatic hepcidin production via induction of Yin Yang 1 [PubMed: 25839654] |
| Blood Purif | Effect of a More Permeable Dialysis Membrane on ESA Resistance in Hemodialysis Patients - A Pilot Investigation [PubMed: 26536083] |
| Asian Journal of Clinical Nutrition | Urinary Hepcidin in Diagnosis of Iron Deficiency Anemia [Openview: E01Ad7C4A9109Ed5Fadb03Ec206D7Bdc] |
| Front Psychol | Ferrous sulfate, but not iron polymaltose complex, aggravates local and systemic inflammation and oxidative stress in dextran sodium sulfate-induced colitis in rats [PubMed: 25852597] |
| Biol Trace Elem Res | Iron Supplementation Attenuates the Inflammatory Status of Anemic Piglets by Regulating Hepcidin [PubMed: 25774043] |
| APS Journals | Kupffer cell depletion protects against the steatosis, but not the liver damage, induced by marginal copper, high fructose diet in male rats. [PubMed: 25813056] |
| European Journal of Haematology | Hepcidin in tumor‐related iron deficiency anemia and tumor‐related anemia of chronic disease: pathogenic mechanisms and diagnosis [PubMed: 24954786] |
| WJA | The Effects of Treatment on Serum Hepcidin and Iron Homeostasis in HIV-1-Infected In-dividuals [Journal: 59107] |
| Nutrition, Metabolism and Cardiovascular Diseases | Hepcidin?and?iron?metabolism?associated?with?cardiometabolic?risk factors?in?children: A case-control?study. [Pubmed:27139516] |
| Food Funct | The effects of polysaccharides from the root of Angelica sinensis on tumor growth and iron metabolism in H22-bearing mice [Pubmed:26757699] |
| Antioxid Redox Signal. | Hydrogen sulfide attenuates inflammatory hepcidin by reducing IL-6 secretion and promoting SIRT1-mediated STAT3 deacetylation [Pubmed:26154696] |
| BioMed Research International | The Impact of Acute Matriptase Inhibition in Hepatic Inflammatory Models. [pubmed:27642598] |
| Frontiers in Neuroscience | In Absence of the Cellular Prion Protein, Alterations in Copper Metabolism and Copper-Dependent Oxidase Activity Affect Iron Distribution. [pubmed:27729845] |
| Experimental Biology and Medicine | Effects of iron supplementation in mice with hypoferremia induced by obesity [pubmed:27439539] |
| Nutrition, Metabolism and Cardiovascular Diseases | Hepcidin and iron metabolism associated with cardiometabolic risk factors in children: A case–control study [pubmed:27139516] |
| PLOS?ONE | S-Propargyl-Cysteine, a?Novel?Hydrogen?Sulfide?Donor,?Inhibits?Inflammatory?Hepcidin?and Relieves Anemia of Inflammation by Inhibiting IL-6/STAT3 Pathway. [pubmed:27649298] |
| Endocrine connections | Hepcidin links gluco-toxicity to pancreatic beta cell dysfunction by inhibiting Pdx-1 expression [pubmed:28179377] |
| Maternal and cord blood hepcidin levels based on gestational weeks in term and preterm infants [publication:305921433_Maternal_and_cord_blood_hepc] | |
| Oxidative Medicine and Cellular Longevity | Acidic Polysaccharide from Angelica sinensis Reverses Anemia of Chronic Disease Involving the Suppression of Inflammatory Hepcidin and NF-κB Activation [pubmed:29147463] |
| The Royal Society of Chemistry | Silkworm feces extract improves iron deficiency anemia via suppressing hepcidin expression and promoting iron-regulatory proteins expression [DOI: 10.1039/C7RA09576G] |
| Journal of Inflammation | Evaluation of a competitive hepcidin ELISA assay in the differential diagnosis of iron deficiency anaemia with concurrent inflammation and anaemia of inflammation in elderly patients [10.1186:s12950-017-0166-3] |
| Antioxidants and Redox Signaling | AMPK serves as a Therapeutic Target against Anemia of Inflammation [pubmed:27923278] |
| NutriciónHospitalaria | Micronutrient supplementation in gastric bypass surgery: prospective study on in??? ammation and iron metabolism in premenopausal women [10.20960/nh.1162] |
| The Japanese Society of Hematology | Hepcidin suppression in β?thalassemia is associated with the down?regulation of atonal homolog 8 [pubmed:28405918] |
| Tuberculosis | Modulation of iron status biomarkers in tuberculosis-diabetes co-morbidity [10.1016/j.tube.2017.11.011] |
| Oman Medical Journal | The Role of Insulin Therapy in Correcting Hepcidin Levels in Patients with Type 2 Diabetes Mellitus. [pubmed:28584599] |
| Cellular?Physiology and Biochemistry | The Effects of Angelica Sinensis Polysaccharide on Tumor Growth and Iron Metabolism by Regulating Hepcidin in Tumor-Bearing Mice [Pubmed:29843136] |
| Cellular?Microbiology? | inhibits ferroportin translation by modulating FBXL5‐IRP2 axis for its growth within host macrophages [Pubmed:29470856] |
| Journal of Histochemistry & Cytochemistry | Acute and Chronic Iron Overloading Differentially Modulates the Expression of Cellular Iron-homeostatic Molecules in Normal Rat Kidney [Pubmed:29873589] |
| Clinical and Experimental?Medicine | Increased NGAL level associated with iron store in chronic kidney disease with anemia [Pubmed:29909502] |
| Hematology | Relationship between obesity and iron deficiency anemia: is there a role of hepcidin? [Pubmed:29318945] |
| Hepcidin serum levels in HCV chronically mono-infected na?ve patients, compared to healthy individuals [77856] | |
| Food & Function | Effect of iron supplement from microalgae on the treatment of iron-deficient anemia in rats [Doi: 10.1039/C8FO01696H] |
| Haematologica | New thiazolidinones reduce iron overload in mouse models of hereditary hemochromatosis and β-thalassemia [Pubmed: 30792208] |
| Ann Intensive Care | Inflammatory anemia-associated parameters are related to 28-day mortality in patients with sepsis admitted to the ICU: a preliminary observational study [Pubmed: 31183575] |
| Journal of Functional Foods | Dietary gelatin enhances non-heme iron absorption possibly via regulation of systemic iron homeostasis in rats [] |
| Naunyn Schmiedebergs Arch Pharmacol. | 3-Amidinophenylalanine-derived matriptase inhibitors can modulate hepcidin production in vitro [Pubmed: 31659405] |
| DEVELOPMENT GROWTH & DIFFERENTIATION | H2O2 induces oxidative stress damage through the BMP‐6/SMAD/hepcidin axis [Pubmed: 32012242] |
| Frontiers in Physiology | Increased Hepcidin Levels During a Period of High Training Load Do Not Alter Iron Status in Male Elite Junior Rowers [Pubmed: 32038278] |
| J Korean Med Sci | Can Calprotectin Show Subclinical Inflammation in Familial Mediterranean Fever Patients? [Pubmed: 32174062] |
| nephrology dialysis transplantation | Iron kinetics following treatment with sucroferric oxyhydroxide or ferric citrate in healthy rats and models of anaemia, iron overload or inflammation [Pubmed: 32259248] |
| mikrochimica acta | Gold-silver core-shell nanoparticle–based impedimetric immunosensor for detection of iron homeostasis biomarker hepcidin [Pubmed: 33095336] |
| JOURNAL OF STEROID BIOCHEMISTRY AND MOLECULAR BIOLOGY | Circulating Osteocyte‐Related Biomarkers (vitamin D, sclerostin, dickkopf-1), hepcidin, and oxidative stress markers in early breast cancer: their impact in?… [Pubmed: 33065276] |
| Sleep Breath | Relation of serum hepcidin levels and restless legs syndrome in chronic hemodialysis patients [Pubmed: 33029690] |
| journal of trace elements in experimental medicine | Difference in iron metabolism may partly explain sex-related variability in the manifestation of Wilson's disease [Pubmed: 32937238] |
| reproduction in domestic animals | Expression of iron regulatory proteins in full term swine placenta [Pubmed: 32449967] |
| Neurobiology of Aging | Estrogen deficiency is associated with brain iron deposition via upregulation of hepcidin expression in aged female mice [Pubmed: 32920472] |
| The Egyptian Journal of Haematology | Urinary hepcidin concentration in assessment of iron homeostasis in pediatrics [] |
| COMPARATIVE MONITORING OF THE ENZYME ACTIVITY OF BONE METABOLISM IN PATIENTS AFTER DENTAL IMPLANTATION [] | |
| Serum iron, prealbumin, D-dimer and prothrombin time are indicators of the diagnosis, severity and outcome of Salmonella enterica serovar Enteritidis infection [] | |
| Гепсидин и индекс фиброза печени MELD-XI как маркеры полиорганной недостаточности у пациентов хронической сердечной недостаточностью с?… [] | |
| Ассоциации уровней NT-proBNP и гепсидина с клинико-лабораторными параметрами у больных хронической сердечной недостаточностью с разной … [] | |
| Biosci Rep | Correlation of hepcidin and serum ferritin levels in thalassemia patients at Chiang Mai University Hospital [33565577] |
| Biometals | Iron metabolism is disturbed and anti-copper treatment improves but does not normalize iron metabolism in Wilson's disease [33555495] |
| J Mol Cell Cardiol | Systemic iron deficiency does not affect the cardiac iron content and progression of heart failure [34139233] |
| Continuous Veno-Venous Hemofiltration (CVVH) Improves Iron Metabolism Disorders in Patients With Sepsis: A Single-Center Prospective Cohort Study [] | |
| Reports of Biochemistry & Molecular Biology | Evaluation of Serum Cyclooxygenase, Hepcidin Levels in Acute Renal Injury (AKI) Patients Following Cardiac Catheterization [] |
| Food Funct | High-dose ferric citrate supplementation attenuates omega-3 polyunsaturated fatty acid biosynthesis via downregulating delta 5 and 6 desaturases in rats with high-fat?… [34787162] |
| J Trace Elem Med Biol | Caffeine and alcohol-friends or foes of human iron stores? [Pubmed:35063815] |
| PeerJ | Resveratrol ameliorates iron overload induced liver fibrosis in mice by regulating iron homeostasis [Pubmed:35698613] |
| Serum Calprotectin and Iron Metabolism Biomarker Levels in Beh?et's Disease [] | |
| Food Bioscience | Roles of homopolymeric apoferritin in alleviating alcohol-induced liver injury [] |
留言咨詢
