組織因子(TF)檢測試劑盒(酶聯(lián)免疫吸附試驗法)
ELISA Kit for Tissue Factor (TF)
CD142; FIII; F3; TFA; Thromboplastin; Coagulation Factor III
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產品包裝(模擬)
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產品包裝(模擬)
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實驗結果圖
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標準曲線圖
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通過ISO 9001、ISO 13485質量體系認證
特異性
本試劑盒用于檢測組織因子(TF)，經檢測與其它相似物質無明顯交叉反應。
由于受到技術及樣本來源的限制，不可能完成對所有相關或相似物質交叉反應檢測，因此本試劑盒有可能與未經檢測的其它物質有交叉反應。
回收率
分別于定值血清及血漿樣本中加入一定量的組織因子(TF)（加標樣品），重復測定并計算其均值，回收率為測定值與理論值的比率。
| 樣本 | 回收率范圍(%) | 平均回收率(%) |
| sodium citrate plasma(n=5) | 97-105 | 101 |
精密度
精密度用樣品測定值的變異系數(shù)CV表示。CV(%) = SD/mean×100
批內差：取同批次試劑盒對低、中、高值定值樣本進行定量檢測,每份樣本連續(xù)測定20 次，分別計算不同濃度樣本的平均值及SD值。
批間差：選取3個不同批次的試劑盒分別對低、中、高值定值樣本進行定量測定，每個樣本使用同一試劑盒重復測定8次，分別計算不同濃度樣本的平均值及SD值。
批內差： CV<10%
批間差： CV<12%
線性
在定值血清及血漿樣本內加入適量的組織因子(TF)，并倍比稀釋成1:2，1:4，1:8，1:16的待測樣本，線性范圍即為稀釋后樣本中組織因子(TF)含量的測定值與理論值的比率。
| 樣本 | 1:2 | 1:4 | 1:8 | 1:16 |
| sodium citrate plasma(n=5) | 80-104% | 80-94% | 93-103% | 91-105% |
穩(wěn)定性
經測定，試劑盒在有效期內按推薦溫度保存，其活性降低率小于5%。
為減小外部因素對試劑盒破壞前后檢測值的影響，實驗室的環(huán)境條件需盡量保持一致，尤其是實驗室內溫度、濕度及溫育條件。其次由同一實驗員來進行操作可減少人為誤差。
實驗流程
1. 實驗前標準品、試劑及樣本的準備；
2. 加樣（標準品及樣本）100µL，37°C孵育1小時；
3. 吸棄，加檢測溶液A100µL，37°C孵育1小時；
4. 洗板3次；
5. 加檢測溶液B100µL，37°C孵育30分鐘；
6. 洗板5次；
7. 加TMB底物90µL，37°C孵育10-20分鐘；
8. 加終止液50µL，立即450nm讀數(shù)。
實驗原理
將組織因子(TF)抗體包被于96孔微孔板中，制成固相載體，向微孔中分別加入標準品或標本，其中的組織因子(TF)與連接于固相載體上的抗體結合，然后加入生物素化的組織因子(TF)抗體，將未結合的生物素化抗體洗凈后，加入HRP標記的親和素，再次徹底洗滌后加入TMB底物顯色。TMB在過氧化物酶的催化下轉化成藍色，并在酸的作用下轉化成最終的黃色。顏色的深淺和樣品中的組織因子(TF)呈正相關。用酶標儀在450nm波長下測定吸光度(O.D.值)，計算樣品濃度。
增值服務
相關產品
| 編號 | 適用物種：Homo sapiens (Human，人) | 應用(僅供研究使用，不用于臨床診斷！) |
| URPA524Hu01 | 組織因子(TF)重組蛋白 | Positive Control; Immunogen; SDS-PAGE; WB. |
| UPAA524Hu01 | 組織因子(TF)多克隆抗體 | WB; IHC; ICC; IP. |
| UFAA524Hu01 | 抗組織因子(TF)多克隆抗體 | Flow cytometry. |
| ULAA524Hu71 | 組織因子(TF)多克隆抗體(生物素標記) | WB; IHC; ICC. |
| ULAA524Hu81 | 組織因子(TF)多克隆抗體(異硫氰酸熒光素標記) | WB; IHC; ICC; IF. |
| UFAA524Hu81 | 抗組織因子(TF)多克隆抗體(異硫氰酸熒光素標記) | FCM |
| UMAA524Hu21 | 組織因子(TF)單克隆抗體 | WB; IHC; ICC/IF |
| UMAA524Hu22 | 組織因子(TF)單克隆抗體 | IHC; ICC/IF |
| UMAA524Hu23 | 組織因子(TF)單克隆抗體 | IHC |
| UFAA524Hu42 | 抗組織因子(TF)單克隆抗體(藻紅素標記) | FCM |
| USEA524Hu | 組織因子(TF)檢測試劑盒(酶聯(lián)免疫吸附試驗法) | Enzyme-linked immunosorbent assay for Antigen Detection. |
| USCA524Hu | 組織因子(TF)檢測試劑盒(化學發(fā)光免疫分析法) | Chemiluminescent immunoassay for Antigen Detection. |
| ULMA524Hu | 組織因子(TF)等多因子檢測試劑盒(流式熒光發(fā)光法) | FLIA Kit for Antigen Detection. |
參考文獻
| 雜志 | 參考文獻 |
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| BioMed research international | Endothelial gene expression and molecular changes in response to radiosurgery in in vitro and in vivo models of cerebral arteriovenous malformations [Pubmed: 24199192] |
| The Journal of Biological Chemistry | Sphingolipid Pathway Regulates Innate Immune Responses at the Fetomaternal Interface during Pregnancy [Pubmed:25505239] |
| Hippokratia | Thrombosis in Hemodialysis Patients; Their Association with Tissue Factor and Tissue Factor Pathway Inhibitor [PubMed: 20011089] |
| shock | Noble-Collip Drum Trauma Induces Disseminated Intravascular Coagulation But Not Acute Coagulopathy of Trauma-Shock [PubMed: 25423126] |
| Perfusion | Ex vivo simulation of cardiopulmonary bypass with human blood for hemocompatibility testing [PubMed: 26243277] |
| Molecular Nutrition & Food Research | Trans‐fatty acid promotes thrombus formation in mice by aggravating antithrombogenic endothelial functions via Toll‐like receptors [PubMed: 25546502] |
| Food Funct | The effects of polysaccharides from the root of Angelica sinensis on tumor growth and iron metabolism in H22-bearing mice [Pubmed:26757699] |
| Blood Cells, Inflammation and Infection | Nebulized Heparin Attenuates Pulmonary Coagulopathy and Inflammation through Alveolar Macrophages in a Rat Model of Acute Lung Injury [pubmed:29202212] |
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