骨保護素(OPG)檢測試劑盒(酶聯(lián)免疫吸附試驗法)
ELISA Kit for Osteoprotegerin (OPG)
TNFRSF11B; TNFRSF11-B; OCIF; OPG; TR1; Tumor Necrosis Factor Receptor Superfamily Member 11b; Osteoclastogenesis Inhibitory Factor
- 編號USEA108Mu
- 物種Mus musculus (Mouse，小鼠) 相同的名稱，不同的物種。
- 實驗方法雙抗夾心
- 反應時長3h
- 檢測范圍0.156-10ng/mL
- 靈敏度最小可檢測劑量小于等于0.060ng/mL.
- 樣本類型serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
- 下載 英文說明書 中文說明書
- 規(guī)格 48T96T 96T*5 96T*10 96T*100
- 價格 ¥ 983 ¥ 1404 ¥ 6318 ¥ 11934 ¥ 98280
- 欲了解實際交易價格和更多情況，請與當地經銷商聯(lián)系!
-
產品包裝(模擬)
-
產品包裝(模擬)
-
實驗結果圖
-
標準曲線圖
-
通過ISO 9001、ISO 13485質量體系認證
特異性
本試劑盒用于檢測骨保護素(OPG)，經檢測與其它相似物質無明顯交叉反應。
由于受到技術及樣本來源的限制，不可能完成對所有相關或相似物質交叉反應檢測，因此本試劑盒有可能與未經檢測的其它物質有交叉反應。
回收率
分別于定值血清及血漿樣本中加入一定量的骨保護素(OPG)（加標樣品），重復測定并計算其均值，回收率為測定值與理論值的比率。
| 樣本 | 回收率范圍(%) | 平均回收率(%) |
| serum(n=5) | 93-105 | 97 |
| EDTA plasma(n=5) | 96-104 | 101 |
| heparin plasma(n=5) | 89-98 | 93 |
精密度
精密度用樣品測定值的變異系數CV表示。CV(%) = SD/mean×100
批內差：取同批次試劑盒對低、中、高值定值樣本進行定量檢測,每份樣本連續(xù)測定20 次，分別計算不同濃度樣本的平均值及SD值。
批間差：選取3個不同批次的試劑盒分別對低、中、高值定值樣本進行定量測定，每個樣本使用同一試劑盒重復測定8次，分別計算不同濃度樣本的平均值及SD值。
批內差： CV<10%
批間差： CV<12%
線性
在定值血清及血漿樣本內加入適量的骨保護素(OPG)，并倍比稀釋成1:2，1:4，1:8，1:16的待測樣本，線性范圍即為稀釋后樣本中骨保護素(OPG)含量的測定值與理論值的比率。
| 樣本 | 1:2 | 1:4 | 1:8 | 1:16 |
| serum(n=5) | 89-96% | 94-101% | 93-103% | 78-98% |
| EDTA plasma(n=5) | 91-101% | 80-101% | 96-103% | 83-102% |
| heparin plasma(n=5) | 88-101% | 83-95% | 94-103% | 95-105% |
穩(wěn)定性
經測定，試劑盒在有效期內按推薦溫度保存，其活性降低率小于5%。
為減小外部因素對試劑盒破壞前后檢測值的影響，實驗室的環(huán)境條件需盡量保持一致，尤其是實驗室內溫度、濕度及溫育條件。其次由同一實驗員來進行操作可減少人為誤差。
實驗流程
1. 實驗前標準品、試劑及樣本的準備；
2. 加樣（標準品及樣本）100µL，37°C孵育1小時；
3. 吸棄，加檢測溶液A100µL，37°C孵育1小時；
4. 洗板3次；
5. 加檢測溶液B100µL，37°C孵育30分鐘；
6. 洗板5次；
7. 加TMB底物90µL，37°C孵育10-20分鐘；
8. 加終止液50µL，立即450nm讀數。
實驗原理
將骨保護素(OPG)抗體包被于96孔微孔板中，制成固相載體，向微孔中分別加入標準品或標本，其中的骨保護素(OPG)與連接于固相載體上的抗體結合，然后加入生物素化的骨保護素(OPG)抗體，將未結合的生物素化抗體洗凈后，加入HRP標記的親和素，再次徹底洗滌后加入TMB底物顯色。TMB在過氧化物酶的催化下轉化成藍色，并在酸的作用下轉化成最終的黃色。顏色的深淺和樣品中的骨保護素(OPG)呈正相關。用酶標儀在450nm波長下測定吸光度(O.D.值)，計算樣品濃度。
增值服務
相關產品
| 編號 | 適用物種：Mus musculus (Mouse，小鼠) | 應用(僅供研究使用，不用于臨床診斷！) |
| UEPA108Mu61 | 骨保護素(OPG)真核蛋白 | Positive Control; Immunogen; SDS-PAGE; WB. |
| URPA108Mu01 | 骨保護素(OPG)重組蛋白 | Positive Control; Immunogen; SDS-PAGE; WB. |
| URPA108Mu02 | 骨保護素(OPG)重組蛋白 | Positive Control; Immunogen; SDS-PAGE; WB. |
| UPAA108Mu01 | 骨保護素(OPG)多克隆抗體 | WB; IHC |
| UPAA108Mu02 | 骨保護素(OPG)多克隆抗體 | WB; IHC; ICC; IP. |
| UMEA108Mu | 骨保護素(OPG)檢測試劑盒(酶聯(lián)免疫吸附試驗法,小樣本) | Enzyme-linked immunosorbent assay for Antigen Detection. |
| USEA108Mu | 骨保護素(OPG)檢測試劑盒(酶聯(lián)免疫吸附試驗法) | Enzyme-linked immunosorbent assay for Antigen Detection. |
| USCA108Mu | 骨保護素(OPG)檢測試劑盒(化學發(fā)光免疫分析法) | Chemiluminescent immunoassay for Antigen Detection. |
| ULMA108Mu | 骨保護素(OPG)等多因子檢測試劑盒(流式熒光發(fā)光法) | FLIA Kit for Antigen Detection. |
| UOGA108Mu02 | 骨保護素(OPG)基因敲除小鼠 | 動物實驗，陰性對照。 |
參考文獻
| 雜志 | 參考文獻 |
| Pharmacology | Protective Effect of Amlodipine on Rat Bone Tissue after Orchidectomy [Karger: 335491] |
| Endocrine Pharmacology | Protective effect of atorvastatin on bone tissue in orchidectomised male albino Wistar rats [ScienceDirect: S0014299912000659] |
| PLOS One | Adipokines in Psoriatic Arthritis Patients: The Correlations with Osteoclast Precursors and Bone Erosions [PubMed: PMC3483160] |
| International Journal of Oral and Maxillofacial Surgery | The effects of chronic zoledronate usage on the jaw and long bones evaluated using RANKL and osteoprotegerin levels in an animal model [Pubmed: 23522850] |
| American Journal of Orthodontics and Dentofacial Orthopedics | OPG and RANKL levels around miniscrew implants during orthodontic tooth movement [Pubmed: 23910201] |
| AGE | Estrogen deficiency does not decrease the in vitro osteogenic potential of rat adipose-derived mesenchymal stem cells [Pubmed: 24687841] |
| Food Science & Nutrition | Effects and mechanisms of 8-prenylnaringenin on osteoblast MC3T3-E1 and osteoclast-like cells RAW264.7 [Onlinelibrary: fsn3.109] |
| Food Sci Nutr. | Effects and mechanisms of 8‐prenylnaringenin on osteoblast MC3T3‐E1 and osteoclast‐like cells RAW264. 7 [Pubmed:Pmc4221832] |
| J Neurol Sci. | The effect of topiramate and lamotrigine on rat bone mass, structure and metabolism. [Pubmed:24629477] |
| Journal of Investigative Surgery | Effect of Nicotine on RANKL and OPG and Bone Mineral Density [Pubmed:24830656] |
| Int J Mol Med. | Effects of icariin on the regulation of the OPG?RANKL?RANK system are mediated through the MAPK pathways in IL?1β?stimulated human SW1353 chondrosarcoma … [Pubmed:25270538] |
| Clinical and Experimental Pharmacology and Physiology | Carvedilol promotes neurological function, reduces bone loss and attenuates cell damage after acute spinal cord injury in rats [Pubmed:25424914] |
| Journal of Colloid and Interface Science | Strontium and zoledronate hydroxyapatites graded composite coatings for bone prostheses [Pubmed:25706198] |
| Int J Clin Exp Pathol | A novel model of bisphosphonate-related osteonecrosis of the jaw in rats [PubMed: 26191212] |
| IJHS(international journal of health sciences) | Relation of Osteoprotegerin, Visfatin and Ghrelin to Metabolic Syndrome in Type 2 Diabetic Patients [PubMed: 26309431] |
| Pharmacology | Effect of Mirtazapine on Rat Bone Tissue after Orchidectomy [PubMed: 25871861] |
| Experimental And Therapeutic Medicine | Antiosteoporotic effect of icariin in ovariectomized rats is mediated via the Wnt/β-catenin pathway [Pubmed:27347050] |
| Bone | The effects of strength training and raloxifene on bone health in aging ovariectomized rats [Pubmed:26812611] |
| Scienze Biomediche | Effetti del laser a bassa dose su cellule osteoblastiche [INCERTIPARENTI_SERENA_TESI.pdf] |
| journal of biomedical materials research part a | RAW 264.7 co-cultured with ultra-high molecular weight polyethylene particles spontaneously differentiate into osteoclasts: an in vitro model of periprosthetic osteolysis. [pubmed:27667508] |
| Nutrition. | Noni leaf and black tea enhance bone regeneration in estrogen-deficient rats. [pubmed:27908549] |
| PLoS One. | Extracorporeal shock waves alone or combined with raloxifene promote bone formation and suppress resorption in ovariectomized rats. [pubmed:28158228] |
| Sci Rep. | Skeletal Site-specific Effects of Zoledronate on in vivo Bone Remodeling and in vitro BMSCs Osteogenic Activity. [pubmed:28139685] |
| Journal of Biomaterials and Tissue Engineering | Modification and Characterization of Polyethylene Glycolated Decellularized Porcine Aortic Heart Valve with Osteoprotegerin [art00007] |
| Japanese Society for Bone and Mineral Research | Allogeneic yet?major histocompatibility complex?matched bone marrow transplantation in?mice results in?an?impairment of?osteoblasts and?a?significantly reduced trabecular bone [article:10.1007] |
| ONCOLOGY LETTERS | Assessment of bone metabolism and biomechanical properties of the femur, following treatment with anastrozole and letrozole in an experimental model of menopause [ol.2017.6596] |
| Journal of Biomedical Materials Research | RAW 264.7 co-cultured with ultra-high molecular weight polyethylene particles spontaneously differentiate into osteoclasts: an in vitro model of periprosthetic osteolysis [10.1002/jbm.a.35912] |
| Oncotarget | Mesenchymal stem cells inhibit RANK-RANKL interactions between osteoclasts and Th17 cells via osteoprotegerin activity. [pubmed:29137353] |
| Asian Journal of Biomedical and Pharmaceutical Sciences | A Water Extract of Benefiting-Bone Capsule Improves Osteoporosis via Transcriptional and Translational Regulation of Key Factors in Notch and Wnt/???2-catenin Signaling Pathways in Ovariectomized Rats [ISSN 2249 – 622X] |
| ACS Applied Materials and Interfaces | Enhanced Osseointegration of Porous Titanium Modified with Zeolitic Imidazolate Framework-8. [pubmed:28696091] |
| Journal of?Clinical?Periodontology? | Peroxisome proliferator‐activated receptor γ plays dual roles on experimental periodontitis in rats [Pubmed:29574908] |
| Journal of Functional Foods | Effect of long-term administration of mangiferin from on bone metabolism in ovariectomized rats [10.1016:j.jff.2018.04.048] |
| Macromolecular?Bioscience | Strontium‐Substituted Hydroxyapatite‐Gelatin Biomimetic Scaffolds Modulate Bone Cell Response [Pubmed:29877029] |
| Journal of Ethnopharmacology | Effects of water extract from epimedium on neuropeptide signaling in an ovariectomized osteoporosis rat model [Pubmed:29705515] |
| International Journal of Molecular Medicine | Bu?Shen?Ning?Xin decoction suppresses osteoclastogenesis by modulating RANKL/OPG imbalance in the CD4+ T lymphocytes of ovariectomized mice [Pubmed:29717766] |
| Journal of?Bone?and?Mineral?Metabolism | Allogeneic yet major histocompatibility complex-matched bone marrow transplantation in mice results in an impairment of osteoblasts and a significantly reduced?… [Pubmed:28766138] |
| Journal of?Bone?and?Mineral?Metabolism? | Therapeutic effect of icariin combined with stem cells on postmenopausal osteoporosis in rats [Pubmed:28681147] |
| Pharmacology | Effects of Amlodipine on Bone Metabolism in Orchidectomised Spontaneously Hypertensive Rats [Pubmed:29898457] |
| Experimental and Therapeutic Medicine | Liraglutide exerts a bone?protective effect in ovariectomized rats with streptozotocin?induced diabetes by inhibiting osteoclastogenesis [Pubmed:29805533] |
| Bone | The sole and combined effect of simvastatin and platelet rich fibrin as a filling material in induced bone defect in tibia of albino rats [Pubmed: 30208342] |
| Bone?Research | NUMB maintains bone mass by promoting degradation of PTEN and GLI1 via ubiquitination in osteoblasts [Pubmed: 30455992] |
| Bone | Development and characterization of supramolecular calcitonin assembly and assessment of its interactions with the bone remodelling process [Pubmed: 30797058] |
| RSC?Advances | Biofunctionalization of decellularized porcine aortic valve with OPG-loaded PCL nanoparticles for anti-calcification [Doi: 10.1039/C9RA00408D] |
| ANTICANCER RESEARCH | Promoter Methylation Down-regulates Osteoprotegerin Expression in Ovarian Carcinoma [Pubmed: 31092428] |
| BIOSCIENCE REPORTS | IL-38 restrains inflammatory response of collagen-induced arthritis in rats via SIRT1/HIF-1α signaling pathway [Pubmed: 32347300] |
| Oxid Med Cell Longev | Long-Term Administration of Abacavir and Etravirine Impairs Semen Quality and Alters Redox System and Bone Metabolism in Growing Male Wistar Rats [34373766] |
| Biomed Pharmacother | Jiangu granule ameliorated OVX rats bone loss by modulating gut microbiota-SCFAs-Treg/Th17 axis [Pubmed:35453007] |
| Medicina (Kaunas) | Plasma Concentrations of New Biochemical Markers of Atherosclerosis in Patients with Dyslipidemia—A Pilot Study [Pubmed:35743980] |
| Journal of Orthopaedic Translation | Exercised accelerated the production of muscle-derived kynurenic acid in skeletal muscle and alleviated the postmenopausal osteoporosis through the Gpr35?… [] |
留言咨詢
