組織金屬蛋白酶抑制因子1(TIMP1)檢測試劑盒(酶聯(lián)免疫吸附試驗法)
ELISA Kit for Tissue Inhibitors Of Metalloproteinase 1 (TIMP1)
EPO; CLGI; EPA; HCI; Erythroid Potentiating Activity; Collagenase Inhibitor; Metalloproteinase inhibitor 1; Fibroblast collagenase inhibitor
- 編號USEA552Mu
- 物種Mus musculus (Mouse，小鼠) 相同的名稱，不同的物種。
- 實驗方法雙抗夾心
- 反應時長3h
- 檢測范圍78-5,000pg/mL
- 靈敏度最小可檢測劑量小于等于28pg/mL.
- 樣本類型serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
- 下載 英文說明書 中文說明書
- 規(guī)格 48T96T 96T*5 96T*10 96T*100
- 價格 ¥ 983 ¥ 1404 ¥ 6318 ¥ 11934 ¥ 98280
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產品包裝(模擬)
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產品包裝(模擬)
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實驗結果圖
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標準曲線圖
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通過ISO 9001、ISO 13485質量體系認證
特異性
本試劑盒用于檢測組織金屬蛋白酶抑制因子1(TIMP1)，經檢測與其它相似物質無明顯交叉反應。
由于受到技術及樣本來源的限制，不可能完成對所有相關或相似物質交叉反應檢測，因此本試劑盒有可能與未經檢測的其它物質有交叉反應。
回收率
分別于定值血清及血漿樣本中加入一定量的組織金屬蛋白酶抑制因子1(TIMP1)（加標樣品），重復測定并計算其均值，回收率為測定值與理論值的比率。
| 樣本 | 回收率范圍(%) | 平均回收率(%) |
| serum(n=5) | 90-98 | 94 |
| EDTA plasma(n=5) | 81-90 | 86 |
| heparin plasma(n=5) | 80-94 | 84 |
精密度
精密度用樣品測定值的變異系數(shù)CV表示。CV(%) = SD/mean×100
批內差：取同批次試劑盒對低、中、高值定值樣本進行定量檢測,每份樣本連續(xù)測定20 次，分別計算不同濃度樣本的平均值及SD值。
批間差：選取3個不同批次的試劑盒分別對低、中、高值定值樣本進行定量測定，每個樣本使用同一試劑盒重復測定8次，分別計算不同濃度樣本的平均值及SD值。
批內差： CV<10%
批間差： CV<12%
線性
在定值血清及血漿樣本內加入適量的組織金屬蛋白酶抑制因子1(TIMP1)，并倍比稀釋成1:2，1:4，1:8，1:16的待測樣本，線性范圍即為稀釋后樣本中組織金屬蛋白酶抑制因子1(TIMP1)含量的測定值與理論值的比率。
| 樣本 | 1:2 | 1:4 | 1:8 | 1:16 |
| serum(n=5) | 83-101% | 82-96% | 81-99% | 90-99% |
| EDTA plasma(n=5) | 87-99% | 95-103% | 92-101% | 91-101% |
| heparin plasma(n=5) | 93-104% | 94-101% | 92-99% | 80-103% |
穩(wěn)定性
經測定，試劑盒在有效期內按推薦溫度保存，其活性降低率小于5%。
為減小外部因素對試劑盒破壞前后檢測值的影響，實驗室的環(huán)境條件需盡量保持一致，尤其是實驗室內溫度、濕度及溫育條件。其次由同一實驗員來進行操作可減少人為誤差。
實驗流程
1. 實驗前標準品、試劑及樣本的準備；
2. 加樣（標準品及樣本）100µL，37°C孵育1小時；
3. 吸棄，加檢測溶液A100µL，37°C孵育1小時；
4. 洗板3次；
5. 加檢測溶液B100µL，37°C孵育30分鐘；
6. 洗板5次；
7. 加TMB底物90µL，37°C孵育10-20分鐘；
8. 加終止液50µL，立即450nm讀數(shù)。
實驗原理
將組織金屬蛋白酶抑制因子1(TIMP1)抗體包被于96孔微孔板中，制成固相載體，向微孔中分別加入標準品或標本，其中的組織金屬蛋白酶抑制因子1(TIMP1)與連接于固相載體上的抗體結合，然后加入生物素化的組織金屬蛋白酶抑制因子1(TIMP1)抗體，將未結合的生物素化抗體洗凈后，加入HRP標記的親和素，再次徹底洗滌后加入TMB底物顯色。TMB在過氧化物酶的催化下轉化成藍色，并在酸的作用下轉化成最終的黃色。顏色的深淺和樣品中的組織金屬蛋白酶抑制因子1(TIMP1)呈正相關。用酶標儀在450nm波長下測定吸光度(O.D.值)，計算樣品濃度。
增值服務
相關產品
| 編號 | 適用物種：Mus musculus (Mouse，小鼠) | 應用(僅供研究使用，不用于臨床診斷！) |
| URPA552Mu02 | 組織金屬蛋白酶抑制因子1(TIMP1)重組蛋白 | Positive Control; Immunogen; SDS-PAGE; WB. |
| URPA552Mu01 | 組織金屬蛋白酶抑制因子1(TIMP1)重組蛋白 | Positive Control; Immunogen; SDS-PAGE; WB. |
| UPAA552Mu01 | 組織金屬蛋白酶抑制因子1(TIMP1)多克隆抗體 | WB; IHC; ICC; IP. |
| UPAA552Mu02 | 組織金屬蛋白酶抑制因子1(TIMP1)多克隆抗體 | WB; IHC; ICC; IP. |
| ULAA552Mu71 | 組織金屬蛋白酶抑制因子1(TIMP1)多克隆抗體(生物素標記) | WB; IHC; ICC. |
| USEA552Mu | 組織金屬蛋白酶抑制因子1(TIMP1)檢測試劑盒(酶聯(lián)免疫吸附試驗法) | Enzyme-linked immunosorbent assay for Antigen Detection. |
| USCA552Mu | 組織金屬蛋白酶抑制因子1(TIMP1)檢測試劑盒(化學發(fā)光免疫分析法) | Chemiluminescent immunoassay for Antigen Detection. |
| ULMA552Mu | 組織金屬蛋白酶抑制因子1(TIMP1)等多因子檢測試劑盒(流式熒光發(fā)光法) | FLIA Kit for Antigen Detection. |
參考文獻
| 雜志 | 參考文獻 |
| Experimental & Translational Stroke Medicine | Early outcome and blood-brain barrier integrity after co-administered thrombolysis and hyperbaric oxygenation in experimental stroke [PubMed: 21679435] |
| PLoS ONE | A Combinatorial Relative Mass Value Evaluation of Endogenous Bioactive Proteins in Three-Dimensional Cultured Nucleus Pulposus Cells of Herniated Intervertebral Discs: Identification of Potential Target Proteins for Gene Therapeutic Approaches [Plosone: Source] |
| Polski Przeglad Chirurgiczny | Concentration of gelatinases and their tissue inhibitors in pancreatic inflammatory and neoplastic tumors and their influence on the early postoperative course [Pubmed: 23585206] |
| Thromb Haemost. | Analysis of the expression of nine secreted matrix metalloproteinases and their endogenous inhibitors in the brain of mice subjected to ischaemic stroke [Pubmed:24671655] |
| The Scientific World Journal | Pass-predicted hepatoprotective activity of Caesalpinia sappan in thioacetamide-induced liver fibrosis in rats [Pubmed:24701154] |
| PLOS ONE | The Complementary Effects of Atorvastatin and Exercise Treatment on the Composition and Stability of the Atherosclerotic Plaques in ApoE Knockout Mice [Pubmed:25264981] |
| Mediators of Inflammation | Metalloproteinases and Their Tissue Inhibitors in Comparison between Different Chronic Pneumopathies in the Horse [Journals: Mi] |
| PLOS | Adiponectin Attenuates Lung Fibroblasts Activation and Pulmonary Fibrosis Induced by Paraquat [PubMed: 25945502] |
| Canadian Journal of Physiology and Pharmacology | Modified Citrus Pectin stops progression of liver fibrosis by inhibiting galactin-3 and inducing apoptosis of stellate cells [Doi: Abs] |
| PLoS One | Silencing of Paternally Expressed Gene 10 Inhibits Trophoblast Proliferation and Invasion [PubMed: 26680220] |
| Kyselov | Влияние диуретиков с антиальдостероновым действием на состояние межклеточного матрикса и раннее постинфарктное ремоделирование левого … [Article: View] |
| Оригинальные исследования / Original researches | матриксу та ранн [Article: Viewfile] |
| Basic Research | CTGF upreCavia (Guinea pig )lation correlates with MMP-9 level in airway remodeling in a murine model of asthma [Journal:27699] |
| J Orthop Res. | Tetrandrine suppresses articular inflammatory response by inhibiting pro-inflammatory factors via NF-κB inactivation. [Pubmed:26748661] |
| Int Wound J. | From varices to venous ulceration: the story of chronic venous disease described by metalloproteinases. [Pubmed:26991748] |
| Am J Pathol.? | Serum Osteopontin as a Novel Biomarker for Muscle Regeneration in Duchenne Muscular Dystrophy. [Pubmed:26963343] |
| Journal of Orthopaedic?Research | Tetrandrine suppresses articular inflammatory response by inhibiting pro‐inflammatory factors via NF‐κB inactivation [pubmed:26748661] |
| Canadian Journal of Veterinary?Research | Evaluation of hyaluronic acid, procollagen type III N-terminal peptide, and tissue inhibitor of matrix metalloproteinase-1 as serum markers of canine hepatic fibrosis [pubmed:27733785] |
| BMC Veterinary Research | Metalloproteinases?and their?inhibitors?are?influenced?by?inhalative?glucocorticoid?therapy?incombination?with?environmental?dust?reduction?in?equine?recurrent airway obstruction. [pubmed:27938355] |
| Journal of Cellular Physiology | Increased Chondrogenic Potential of Mesenchymal Cells From Adipose Tissue Versus Bone Marrow‐Derived Cells in Osteoarthritic In Vitro Models [pubmed:27739057] |
| Toxicology and Applied Pharmacology | Sildenafil protects against bile duct ligation induced hepaticfibrosis in rats: Potential role for silent information regulator 1 (SIRT1) [pubmed:28974454] |
| Journal of Applied Animal Research | Hypoxia enhances secretion of matrix metalloproteinases in bovine dermal fibroblasts:an in vitro approach to bovine digital dermatitis [10.108:09712119.2017.1335208] |
| Cellular Physiology and Biochemistry | The MiR-495/Annexin A3/P53 Axis Inhibits the Invasion and EMT of Colorectal Cancer Cells [pubmed:29224019] |
| International Wound Journal | From varices to venous ulceration: the story of chronic venous disease described by metalloproteinases [pubmed:26991748] |
| Archives of Medical Science | CTGF upregulation correlates with MMP-9 level in airway remodeling in a murine model of asthma. [pubmed:28507585] |
| APMIS | Hepatoprotectivity of Panduratin A against liver damage: demonstration with a rat model of cirrhosis induced by thioacetamide [Pubmed:30058214] |
| Journal of Applied Animal Research? | Hypoxia enhances secretion of matrix metalloproteinases in bovine dermal fibroblasts: an approach to bovine digital dermatitis [10.1080:09712119.2017.1335208] |
| МАТРИКСНЫЕ МЕТАЛЛОПРОТЕИНАЗЫ: ЗНАЧЕНИЕ В РЕМОДЕЛИРОВАНИИ СЕРДЦА У ПАЦИЕНТОВ С ДИСПЛАЗИЕЙ СОЕДИНИТЕЛЬНОЙ ТКАНИ [] | |
| Mediators of Inflammation | Metalloproteinases and their Inhibitors under the Course of Immunostimulation by CPG-ODN and Specific Antigen Inhalation in Equine Asthma [] |
| International Journal of Clinical and Experimental Medicine | Expression of MMP-9 and TIMP-1 in rat models of pressure ulcer and their significance [] |
| Oncology Research | MicroRNA-6884-5p regulates the proliferation, invasion and EMT of gastric cancer cells by directly targeting S100A16 [Pubmed: 31796150] |
| Open Medicine | Plasma matrix metalloproteinase-9 and tissue inhibitor of matrix metalloproteinase-1 as prognostic biomarkers in critically ill patients [Pubmed: 32190734] |
| Scientific Reports | Matrix metallopeptidase expression and modulation by transforming growth factor-β1 in equine endometrosis [Pubmed: 31980722] |
| JOURNAL OF THROMBOSIS AND THROMBOLYSIS | Extracellular matrix collagen biomarkers levels in patients with chronic thromboembolic pulmonary hypertension [Pubmed: 33175289] |
| Molecular Medicine Reports | MicroRNA-1 promotes cartilage matrix synthesis and regulates chondrocyte differentiation via post-transcriptional suppression of Ihh expression [Pubmed: 32705199] |
| International Journal of Environmental Research and Public Health | Biomarkers Facilitate the Assessment of Prognosis in Critically Ill Patients with Primary Brain Injury: A Cohort Study [Pubmed: 32575870] |
| . | Почечная дисфункция при преэклампсии: прогнозирование и дифференциальный диагноз. Часть 1 [] |
| MEDIATORS OF INFLAMMATION | MMP-9 Concentration in Peritoneal Fluid Is a Valuable Biomarker Associated with Endotoxemia in Equine Colic [33488296] |
| CLINICAL SCIENCE | Berberine accelerated wound healing by restoring TrxR1/JNK in diabetes [33491733] |
| DOCSBAY | Intermittent Pacing Therapy Favorably Modulates Infarct Remodeling [28386775] |
| EVALUATION OF DYNAMICS OF METALLOPROTEINASE ACTIVITY AND INDICATORS OF OXIDATIVE STRESS IN THE TREATMENT OF BURNS WITH A NEW DERIVATIVE OF N-ACETYL-6- AMINOHEXANOIC ACID [] | |
| VETERINARY OPHTHALMOLOGY | Concentrations of tissue inhibitor of matrix metalloproteinase‐1 and hyaluronic acid in canine amniotic membranes cryopreserved for different time points and its?… [34240563] |
| Hum Brain Mapp | Deep transfer learning of structural magnetic resonance imaging fused with blood parameters improves brain age prediction [34913545] |
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