吲哚胺2,3-雙加氧酶(IDO)檢測(cè)試劑盒(酶聯(lián)免疫吸附試驗(yàn)法)
ELISA Kit for Indoleamine-2,3-Dioxygenase (IDO)
CD107B; INDO; Indoleamine-Pyrrole 2,3 Dioxygenase
- 編號(hào)USEB547Ra
- 物種Rattus norvegicus (Rat，大鼠) 相同的名稱，不同的物種。
- 實(shí)驗(yàn)方法雙抗夾心
- 反應(yīng)時(shí)長(zhǎng)3h
- 檢測(cè)范圍0.156-10ng/mL
- 靈敏度最小可檢測(cè)劑量小于等于0.057ng/mL.
- 樣本類型Serum, plasma, tissue homogenates and other biological fluids
- 下載 英文說(shuō)明書 中文說(shuō)明書
- 規(guī)格 48T96T 96T*5 96T*10 96T*100
- 價(jià)格 ¥ 1516 ¥ 2166 ¥ 9747 ¥ 18411 ¥ 151620
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產(chǎn)品包裝(模擬)
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產(chǎn)品包裝(模擬)
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實(shí)驗(yàn)結(jié)果圖
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標(biāo)準(zhǔn)曲線圖
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通過(guò)ISO 9001、ISO 13485質(zhì)量體系認(rèn)證
特異性
本試劑盒用于檢測(cè)吲哚胺2,3-雙加氧酶(IDO)，經(jīng)檢測(cè)與其它相似物質(zhì)無(wú)明顯交叉反應(yīng)。
由于受到技術(shù)及樣本來(lái)源的限制，不可能完成對(duì)所有相關(guān)或相似物質(zhì)交叉反應(yīng)檢測(cè)，因此本試劑盒有可能與未經(jīng)檢測(cè)的其它物質(zhì)有交叉反應(yīng)。
回收率
分別于定值血清及血漿樣本中加入一定量的吲哚胺2,3-雙加氧酶(IDO)（加標(biāo)樣品），重復(fù)測(cè)定并計(jì)算其均值，回收率為測(cè)定值與理論值的比率。
| 樣本 | 回收率范圍(%) | 平均回收率(%) |
| serum(n=5) | 86-95 | 92 |
| EDTA plasma(n=5) | 97-104 | 101 |
| heparin plasma(n=5) | 96-105 | 101 |
精密度
精密度用樣品測(cè)定值的變異系數(shù)CV表示。CV(%) = SD/mean×100
批內(nèi)差：取同批次試劑盒對(duì)低、中、高值定值樣本進(jìn)行定量檢測(cè),每份樣本連續(xù)測(cè)定20 次，分別計(jì)算不同濃度樣本的平均值及SD值。
批間差：選取3個(gè)不同批次的試劑盒分別對(duì)低、中、高值定值樣本進(jìn)行定量測(cè)定，每個(gè)樣本使用同一試劑盒重復(fù)測(cè)定8次，分別計(jì)算不同濃度樣本的平均值及SD值。
批內(nèi)差： CV<10%
批間差： CV<12%
線性
在定值血清及血漿樣本內(nèi)加入適量的吲哚胺2,3-雙加氧酶(IDO)，并倍比稀釋成1:2，1:4，1:8，1:16的待測(cè)樣本，線性范圍即為稀釋后樣本中吲哚胺2,3-雙加氧酶(IDO)含量的測(cè)定值與理論值的比率。
| 樣本 | 1:2 | 1:4 | 1:8 | 1:16 |
| serum(n=5) | 82-102% | 80-90% | 81-96% | 91-101% |
| EDTA plasma(n=5) | 92-99% | 94-103% | 81-103% | 83-99% |
| heparin plasma(n=5) | 84-92% | 82-95% | 97-105% | 95-102% |
穩(wěn)定性
經(jīng)測(cè)定，試劑盒在有效期內(nèi)按推薦溫度保存，其活性降低率小于5%。
為減小外部因素對(duì)試劑盒破壞前后檢測(cè)值的影響，實(shí)驗(yàn)室的環(huán)境條件需盡量保持一致，尤其是實(shí)驗(yàn)室內(nèi)溫度、濕度及溫育條件。其次由同一實(shí)驗(yàn)員來(lái)進(jìn)行操作可減少人為誤差。
實(shí)驗(yàn)流程
1. 實(shí)驗(yàn)前標(biāo)準(zhǔn)品、試劑及樣本的準(zhǔn)備；
2. 加樣（標(biāo)準(zhǔn)品及樣本）100µL，37°C孵育1小時(shí)；
3. 吸棄，加檢測(cè)溶液A100µL，37°C孵育1小時(shí)；
4. 洗板3次；
5. 加檢測(cè)溶液B100µL，37°C孵育30分鐘；
6. 洗板5次；
7. 加TMB底物90µL，37°C孵育10-20分鐘；
8. 加終止液50µL，立即450nm讀數(shù)。
實(shí)驗(yàn)原理
將吲哚胺2,3-雙加氧酶(IDO)抗體包被于96孔微孔板中，制成固相載體，向微孔中分別加入標(biāo)準(zhǔn)品或標(biāo)本，其中的吲哚胺2,3-雙加氧酶(IDO)與連接于固相載體上的抗體結(jié)合，然后加入生物素化的吲哚胺2,3-雙加氧酶(IDO)抗體，將未結(jié)合的生物素化抗體洗凈后，加入HRP標(biāo)記的親和素，再次徹底洗滌后加入TMB底物顯色。TMB在過(guò)氧化物酶的催化下轉(zhuǎn)化成藍(lán)色，并在酸的作用下轉(zhuǎn)化成最終的黃色。顏色的深淺和樣品中的吲哚胺2,3-雙加氧酶(IDO)呈正相關(guān)。用酶標(biāo)儀在450nm波長(zhǎng)下測(cè)定吸光度(O.D.值)，計(jì)算樣品濃度。
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相關(guān)產(chǎn)品
| 編號(hào) | 適用物種：Rattus norvegicus (Rat，大鼠) | 應(yīng)用(僅供研究使用，不用于臨床診斷！) |
| URPB547Ra01 | 吲哚胺2,3-雙加氧酶(IDO)重組蛋白 | Positive Control; Immunogen; SDS-PAGE; WB. |
| URPB547Ra02 | 吲哚胺2,3-雙加氧酶(IDO)重組蛋白 | Positive Control; Immunogen; SDS-PAGE; WB. |
| UPAB547Ra01 | 吲哚胺2,3-雙加氧酶(IDO)多克隆抗體 | WB; IHC |
| UPAB547Ra02 | 吲哚胺2,3-雙加氧酶(IDO)多克隆抗體 | WB; IHC |
| ULAB547Ra71 | 吲哚胺2,3-雙加氧酶(IDO)多克隆抗體(生物素標(biāo)記) | WB |
| UMAB547Ra21 | 吲哚胺2,3-雙加氧酶(IDO)單克隆抗體 | WB; IHC; ICC; IP. |
| USEB547Ra | 吲哚胺2,3-雙加氧酶(IDO)檢測(cè)試劑盒(酶聯(lián)免疫吸附試驗(yàn)法) | Enzyme-linked immunosorbent assay for Antigen Detection. |
| ULMB547Ra | 吲哚胺2,3-雙加氧酶(IDO)等多因子檢測(cè)試劑盒(流式熒光發(fā)光法) | FLIA Kit for Antigen Detection. |
參考文獻(xiàn)
| 雜志 | 參考文獻(xiàn) |
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| Biochemistry | Mechanisms of Leptin and Ghrelin Action on Maturation and Functions of Dendritic Cells [] |
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| Transl Psychiatry | Associations between expression of indoleamine 2, 3-dioxygenase enzyme and inflammatory cytokines in patients with first-episode drug-naive?… [34802039] |
