白介素17(IL17)檢測試劑盒(酶聯(lián)免疫吸附試驗法)
ELISA Kit for Interleukin 17 (IL17)
IL17A; CTLA8; IL-17A; Cytotoxic T-Lymphocyte-Associated Protein 8
- 編號USEA063Ra
- 物種Rattus norvegicus (Rat，大鼠) 相同的名稱，不同的物種。
- 實驗方法雙抗夾心
- 反應(yīng)時長3h
- 檢測范圍15.6-1,000pg/mL
- 靈敏度最小可檢測劑量小于等于6.4pg/mL.
- 樣本類型serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
- 下載 英文說明書 中文說明書
- 規(guī)格 48T96T 96T*5 96T*10 96T*100
- 價格 ¥ 1436 ¥ 2052 ¥ 9234 ¥ 17442 ¥ 143640
- 欲了解實際交易價格和更多情況，請與當(dāng)?shù)亟?jīng)銷商聯(lián)系!
-
產(chǎn)品包裝(模擬)
-
產(chǎn)品包裝(模擬)
-
實驗結(jié)果圖
-
標(biāo)準(zhǔn)曲線圖
-
通過ISO 9001、ISO 13485質(zhì)量體系認(rèn)證
特異性
本試劑盒用于檢測白介素17(IL17)，經(jīng)檢測與其它相似物質(zhì)無明顯交叉反應(yīng)。
由于受到技術(shù)及樣本來源的限制，不可能完成對所有相關(guān)或相似物質(zhì)交叉反應(yīng)檢測，因此本試劑盒有可能與未經(jīng)檢測的其它物質(zhì)有交叉反應(yīng)。
回收率
分別于定值血清及血漿樣本中加入一定量的白介素17(IL17)（加標(biāo)樣品），重復(fù)測定并計算其均值，回收率為測定值與理論值的比率。
| 樣本 | 回收率范圍(%) | 平均回收率(%) |
| serum(n=5) | 78-93 | 83 |
| EDTA plasma(n=5) | 78-90 | 85 |
| heparin plasma(n=5) | 94-105 | 98 |
精密度
精密度用樣品測定值的變異系數(shù)CV表示。CV(%) = SD/mean×100
批內(nèi)差：取同批次試劑盒對低、中、高值定值樣本進(jìn)行定量檢測,每份樣本連續(xù)測定20 次，分別計算不同濃度樣本的平均值及SD值。
批間差：選取3個不同批次的試劑盒分別對低、中、高值定值樣本進(jìn)行定量測定，每個樣本使用同一試劑盒重復(fù)測定8次，分別計算不同濃度樣本的平均值及SD值。
批內(nèi)差： CV<10%
批間差： CV<12%
線性
在定值血清及血漿樣本內(nèi)加入適量的白介素17(IL17)，并倍比稀釋成1:2，1:4，1:8，1:16的待測樣本，線性范圍即為稀釋后樣本中白介素17(IL17)含量的測定值與理論值的比率。
| 樣本 | 1:2 | 1:4 | 1:8 | 1:16 |
| serum(n=5) | 91-98% | 98-105% | 78-99% | 92-101% |
| EDTA plasma(n=5) | 78-103% | 78-92% | 83-99% | 98-105% |
| heparin plasma(n=5) | 87-94% | 80-97% | 94-105% | 94-101% |
穩(wěn)定性
經(jīng)測定，試劑盒在有效期內(nèi)按推薦溫度保存，其活性降低率小于5%。
為減小外部因素對試劑盒破壞前后檢測值的影響，實驗室的環(huán)境條件需盡量保持一致，尤其是實驗室內(nèi)溫度、濕度及溫育條件。其次由同一實驗員來進(jìn)行操作可減少人為誤差。
實驗流程
1. 實驗前標(biāo)準(zhǔn)品、試劑及樣本的準(zhǔn)備；
2. 加樣（標(biāo)準(zhǔn)品及樣本）100µL，37°C孵育1小時；
3. 吸棄，加檢測溶液A100µL，37°C孵育1小時；
4. 洗板3次；
5. 加檢測溶液B100µL，37°C孵育30分鐘；
6. 洗板5次；
7. 加TMB底物90µL，37°C孵育10-20分鐘；
8. 加終止液50µL，立即450nm讀數(shù)。
實驗原理
將白介素17(IL17)抗體包被于96孔微孔板中，制成固相載體，向微孔中分別加入標(biāo)準(zhǔn)品或標(biāo)本，其中的白介素17(IL17)與連接于固相載體上的抗體結(jié)合，然后加入生物素化的白介素17(IL17)抗體，將未結(jié)合的生物素化抗體洗凈后，加入HRP標(biāo)記的親和素，再次徹底洗滌后加入TMB底物顯色。TMB在過氧化物酶的催化下轉(zhuǎn)化成藍(lán)色，并在酸的作用下轉(zhuǎn)化成最終的黃色。顏色的深淺和樣品中的白介素17(IL17)呈正相關(guān)。用酶標(biāo)儀在450nm波長下測定吸光度(O.D.值)，計算樣品濃度。
增值服務(wù)
相關(guān)產(chǎn)品
| 編號 | 適用物種：Rattus norvegicus (Rat，大鼠) | 應(yīng)用(僅供研究使用，不用于臨床診斷！) |
| URPA063Ra01 | 白介素17(IL17)重組蛋白 | Positive Control; Immunogen; SDS-PAGE; WB. |
| UPAA063Ra01 | 白介素17(IL17)多克隆抗體 | WB; IHC; ICC; IP. |
| UMAA063Ra22 | 白介素17(IL17)單克隆抗體 | WB; IHC |
| UMAA063Ra23 | 白介素17(IL17)單克隆抗體 | IHC |
| UMAA063Ra21 | 白介素17(IL17)單克隆抗體 | WB; IHC |
| USEA063Ra | 白介素17(IL17)檢測試劑盒(酶聯(lián)免疫吸附試驗法) | Enzyme-linked immunosorbent assay for Antigen Detection. |
| UMEA063Ra | 白介素17(IL17)檢測試劑盒(酶聯(lián)免疫吸附試驗法,小樣本) | Enzyme-linked immunosorbent assay for Antigen Detection. |
| ULMA063Ra | 白介素17(IL17)等多因子檢測試劑盒(流式熒光發(fā)光法) | FLIA Kit for Antigen Detection. |
參考文獻(xiàn)
| 雜志 | 參考文獻(xiàn) |
| PLOS ONE | Impaired Autoimmune T Helper 17 Cell Responses Following DNA Vaccination against Rat Experimental Autoimmune Encephalomyelitis [PubMed: PMC2577303] |
| Cellular Immunology | Atorvastatin ameliorates experimental autoimmune neuritis by decreased Th1/Th17 cytokines and up-regulated T regulatory cells [ScienceDirect: S0008874911002164] |
| The Journal of Urology | Improvement by Phytotherapeutic Agent of Detrusor Overactivity, Down-Regulation of Pharmacological Receptors and Urinary Cytokines in Rats with Cyclophosphamide Induced Cystitis [PubMed: S0022534712049087] |
| Critical Care Medicine | Systemic release of cytokines and heat shock proteins in porcine models of polytrauma and hemorrhage [PubMed: PMC3383056] |
| Lower Urinary Tract Symptoms | Effects of Saw Palmetto Extract on Urodynamic Parameters, Bladder Muscarinic and Purinergic Receptors and Urinary Cytokines in Rats with Cyclophosphamide-Induced Cystitis [Onlinelibrary: luts.12015] |
| Shock | Expression of Hemopexin in Acute Rejection of Rat Liver Allograft Identified by Serum Proteomic Analysis. [Lww: Source] |
| J Vet Med Sci. | CD4+ T Cell Cytokine Gene and Protein Expression in Duodenal Mucosa of Dogs with Inflammatory Bowel Disease [Pubmed:24270804] |
| Journal of Dairy Science | Increased blood-circulating interferon-γ, interleukin-17, and osteopontin levels in bovine paratuberculosis [Pubmed:24679935] |
| Inflammation.? | Pemetrexed Ameliorates Experimental Arthritis in Rats [Pubmed:25129058] |
| Inflammation | Lapatinib Ameliorates Experimental Arthritis in Rats [Pubmed:25283886] |
| Molecular and Cellular Biochemistry | Beta-arrestin-2 negatively modulates inflammation response in mouse chondrocytes induced by 4-mer hyaluronan oligosaccharide [Pubmed:25318610] |
| Molecular Medicine Reports | Function of interleukin?17 and ?35 in the blood of patients with hepatitis B?related liver cirrhosis [Pubmed:25323532] |
| Genet Mol Res | Protective effect and mechanism of probucol in the treatment of spinal cord injury [PubMed: 26214485] |
| veterinary research forum | Evaluation of proliferation and cytokines production by mitogen-stimulated bovine peripheral blood mononuclear cells [Article_14936_6.Html: ] |
| Tumor Biology | Hypoxia-induced secretion of IL-20 from adipose-derived mesenchymal stem cell promotes growth and cancer stem cell properties of Burkitt lymphoma [PubMed: 26695151] |
| Clinical & Experimental Immunology | MicroRNA‐155 may be involved in the pathogenesis of atopic dermatitis by modulating the differentiation and function of T helper type 17 (Th18) cells [PubMed: 25761610] |
| J Reprod Immunol | The immunomodulating effect of seminal plasma on T cells [PubMed: 25799173] |
| Biomarkers | Interleukin-17 levels correlate with poor prognosis and vascular endothelial growth factor concentration in the serum of patients with non-small cell lung cancer [PubMed: 26301881] |
| Molecular Medicine Reports | Tanshinone IIA attenuates experimental autoimmune encephalomyelitis in rats [Pubmed:27357729] |
| SpringerPlus | Ethyl pyruvate?attenuated?coxsackievirus?B3-induced?acute?viral?myocarditis?by?suppression?ofHMGB1/RAGE/NF-ΚB?pathway. [Pubmed:27026909] |
| International Immunopharmacology | Consistency and pathophysiological characterization of a rat polymicrobial sepsis model via the improved cecal ligation and puncture surgery [Pubmed:26802602] |
| Journal of Agricultural Biotechnology | Gln Attenuates LPS-induced Inflammatory Injury of Bos taurus Testis Sustentacular Cells [10610] |
| Veterinary Research Communications | Immunological and pathological investigations in equine experimental uveitis [pubmed:27344152] |
| Journal of Neuroinflammation | Th17-skewed immune response and cluster of differentiation 40 ligand expression in canine steroid-responsive meningitis-arteritis, a large animal model for neutrophilic meningitis. [pubmed:28114998] |
| American Journal of Translational Research | Anti-inflammatory effects of Boletus edulis polysaccharide on asthma pathology [pubmed:27830033] |
| Springerplus | Ethyl pyruvate attenuated coxsackievirus B3-induced acute viral myocarditis by suppression of HMGB1/RAGE/NF-ΚB pathway [pubmed:27026909] |
| Journal?of?Virology | Characterization?of?Simian?Immunodeficiency?Virus?Variants?Anatomically?Compartmentalized?inPlasma?and?Milk?in?Chronically?Infected?African?Green?Monkeys. [pubmed:] |
| J Nephropathol | Relationship between IL-17 serum level and ambulatory blood pressure in women with polycystic ovary syndrome [pubmed:28042549] |
| Journal of Nephropathology | Relationship between IL-17 and ambulatory blood pressure in polycystic ovary syndrome [8b168a09f5832df1b5c2f48631612458b2d8.pdf] |
| Basic Clin Pharmacol Toxicol. | Magnolol Attenuates Concanavalin A-induced Hepatic Fibrosis, Inhibits CD4+ T Helper 17 (Th17) Cell Differentiation and Suppresses Hepatic Stellate Cell Activation: Blockade of Smad3/Smad4 Signalling. [pubmed:28032440] |
| Gut Pathog. | Oral administration of Clostridium butyricum CGMCC0313‐1 reduces ovalbumin‐induced allergic airway inflammation in mice [pubmed:28250847] |
| Procalcitonin und proinflammatorische Zytokine in der bronchoalveol?ren Lavage beim lungenkranken Pferd unter inhalativer Glukokortikoidtherapie [FUDISS_thesis_000000103830] | |
| Scientific Report | Anti-asthmatic efect of pitavastatin through aerosol inhalation is associated with CD4+ CD25+ Foxp3+ T cells in an asthma mouse model [pubmed:28729731] |
| Basic & Clinical Pharmacology & Toxicology | Magnolol Attenuates Concanavalin A-induced Hepatic Fibrosis, Inhibits CD4 + T Helper 17(Th17) Cell Differentiation and Suppresses Hepatic Stellate Cell Activation: Blockade of Smad3/Smad4 Signalling [doi:10.1111] |
| Academic Journal | Antiinflammatory and antioxidant effects of gemcitabine in collagen-induced arthritis model [pubmed:28618762] |
| International Journal of Clinical and Experimental Pathology | Low-density neutrophils in severe fever with thrombocytopenia syndrome (SFTS) display decreased function to phagocytose SFTS virus and enhanced capacity to synthesize cytokines [ISSN:1936-2625/IJCEP0045334] |
| Asian Pacific Society of Respirology | Oral administration of Clostridium butyricum CGMCC0313-1 reduces ovalbumin-induced allergic airway inflammation in mice [pubmed:28122397] |
| MYOPAIN | Increased Levels of IL-17, IL-23, MIP-1α, MCP-1 and Global Leukocytes in Fibromyalgia Patients [10.1080/24708593.2017.1357664] |
| Molecular?Medicine?Reports | Lower level of IL?35 and its reduced inhibition in Th17 cells in patients with bone marrow mononuclear cells Coombs test?positive hemocytopenia. [pubmed:29257310] |
| Molecular Medicine Reports | Interleukin-17 antagonist attenuates lung inflammation through inhibition of the ERK1/2 and NF-κB pathway in LPS-induced acute lung injury [10.3892/mmr.2017.6837] |
| ARTIFICIAL?CELLS?NANOMEDICINE AND BIOTECHNOLOGY? | Dexamethasone reduces serum level of IL-17 in Bleomycin-A5-induced rats model of pulmonary fibrosis [pubmed:28608724] |
| International Immunopharmacology | Therapeutic effect of bone marrow mesenchymal stem cells pretreated with acetylsalicylic acid on experimental periodontitis in rats [pubmed:29195233] |
| Biomedical Research | Associations among interleukin-17, interferon-gamma, and acute coronary syndrome [ISSN 0970-938X] |
| Cellular Physiology and Biochemistry | Activated Α7nachr Improves Postoperative Cognitive Dysfunction and Intestinal Injury Induced by Cardiopulmonary Bypass in Rats: Inhibition of the Proinflammatory?… [Pubmed:29672286] |
| Frontiers in Pharmacology | Traditional Herbal Medicine-Derived Sulforaphene LFS-01 Reverses Colitis in Mice by Selectively Altering the Gut Microbiota and Promoting Intestinal?… [Pubmed:29375374] |
| Bioscience?Reports | Decreased miR-128 expression alleviated the progression of Rheumatoid arthritis by up-regulating the expression of TNFAIP3 [Pubmed:29853534] |
| Medical?Science?Monitor | Involvement of T-Helper 9 Activation in a Mouse Model of Allergic Rhinitis [Pubmed:29982264] |
| Journal of?International?Medical?Research | Changes in Th1/Th2-producing cytokines during acute exacerbation chronic obstructive pulmonary disease [Pubmed:29950127] |
| Canadian Journal of Physiology and Pharmacology | Inhibition of microRNA-155 attenuates concanavalin A-induced autoimmune hepatitis by regulating Treg/Th17 cell differentiation [Pubmed: 30290123] |
| BMC?Microbiology | Nystatin enhances the immune response against Candida albicans and protects the ultrastructure of the vaginal epithelium in a rat model of vulvovaginal?… [Pubmed: 30359236] |
| Biology?of?Reproduction | Recombinant adiponectin alleviates abortion in mice by regulating Th17/Treg imbalance via p38MAPK-STAT5 pathway [Pubmed: 30496353] |
| BMC?MICROBIOLOGY | Enhanced antifungal activity of bovine lactoferrin-producing probiotic Lactobacillus casei in the murine model of vulvovaginal candidiasis [Pubmed: 30621597] |
| CNS?Neuroscience & Therapeutics | Negative regulation of glial Tim‐3 inhibits the secretion of inflammatory factors and modulates microglia to antiinflammatory phenotype after experimental intracerebral?… [Pubmed: 30677253] |
| OPEN ACCESS MACEDONIAN JOURNAL OF MEDICAL SCIENCES | Seluang Fish (Rasbora Spp.) Oil Decreases Inflammatory Cytokines Via Increasing Vitamin D Level in Systemic Lupus Erythematosus [] |
| Frontiers in Microbiology | Local probiotic Lactobacillus crispatus and Lactobacillus delbrueckii exhibit strong antifungal effects against vulvovaginal candidiasis in a rat model [] |
| 中國免疫學(xué)雜志 | TRAF-6 調(diào)控TGF-β1-Smad2/Smad3 信號通路在Graves 病免疫發(fā)病機(jī)制中的作用 [] |
| Microbial Pathogenesis | Therapeutic effectiveness of type I interferon in vulvovaginal candidiasis [Pubmed: 31158491] |
| Inflammation | Protein Kinase C Theta Inhibition Attenuates Lipopolysaccharide-Induced Acute Lung Injury through Notch Signaling Pathway via Suppressing Th17 Cell Response?… [Pubmed: 31297750] |
| international immunopharmacology | Adenovirus-mediated down-regulation of miR-21-5p alleviates experimental autoimmune uveoretinitis in mice [Pubmed: 31228813] |
| Untersuchung einer Th17-vermittelten Immunantwort bei der kaninen idiopathischen Epilepsie [] | |
| Free Radical Biology and Medicine | Vitamin D protects against oxidative stress, inflammation and hepatorenal damage induced by acute paracetamol toxicity in rat [Pubmed: 31255668] |
| BioMed Research International | Indoleamine 2, 3-Dioxygenase (IDO) Regulates Th17/Treg Immunity in Experimental IgA Nephropathy [] |
| Animals | Anti-Inflammatory State in Arabian Horses Introduced to the Endurance Training [Pubmed: 31462005] |
| World Journal of Pediatrics | Regulation of JAK/STAT signal pathway by miR-21 in the pathogenesis of juvenile idiopathic arthritis [Pubmed: 31641939] |
| Biotechnology and Applied Biochemistry | Epigallocatechin 3‐gallate attenuates arthritis by regulating Nrf‐2, HO‐1 and cytokine levels in an experimental arthritis model [Pubmed: 31746064] |
| Microbial Pathogenesis | Tangeretin attenuates lipopolysaccharide-induced acute lung injury through Notch signaling pathway via suppressing Th17 cell response in mice [Pubmed: 31676364] |
| International Journal of Rheumatic Diseases | Effects of thalidomide on Th17, Treg cells and TGF‐β1/Smad3 pathway in a mouse model of systemic sclerosis [Pubmed: 31840939] |
| Cytokine | Levels of pro-and anti-inflammatory cytokines in cystic fibrosis patients with or without gingivitis [Pubmed: 31927460] |
| international urology and?nephrology | The therapeutic effect of dendritic cells expressing indoleamine 2, 3-dioxygenase (IDO) on an IgA nephropathy mouse model [Pubmed: 31894556] |
| JOURNAL OF PERIODONTAL RESEARCH | Salivary Del‐1, IL‐17, and LFA‐1 levels in periodontal health and disease [Pubmed: 32153040] |
| journal of molecular endocrinology | LncRNA Profile in Hashimoto's Thyroiditis and Potential Function of NONHSAT079547. 2 [Pubmed: 32229697] |
| Toxicol Appl Pharmacol | Daphnetin ameliorates glucocorticoid-induced osteoporosis via activation of Wnt/GSK-3β/β-catenin signaling [Pubmed: 33171191] |
| CARDIOVASCULAR?DRUGS?AND THERAPY | Rhodiola crenulata reduces ventricular arrhythmia through mitigating the activation of IL-17 and inhibiting the MAPK signaling pathway [Pubmed: 32946036] |
| Biomed Research International | Effects of 2, 3, 7, 8-Tetrachlorodibenzo-p-dioxin on T Cell Differentiation in Primary Biliary Cholangitis [Pubmed: 32908870] |
| J Neurosurg | Covering the proximal nerve stump with chondroitin sulfate proteoglycans prevents traumatic painful neuroma formation by blocking axon regeneration after … [Pubmed: 32470939] |
| JOURNAL OF MICROBIOLOGY | Melatonin attenuates microbiota dysbiosis of jejunum in short-term sleep deprived mice [Pubmed: 32424577] |
| bioscience reports | Protective effect of Asarum sieboldii essential oil on ovalbumin induced allergic rhinitis in rat [Pubmed: 32395767] |
| Journal of Biochemical and Molecular Toxicology | Vitamin D3 and calcium cosupplementation alleviates cadmium hepatotoxicity in the rat: Enhanced antioxidative and anti‐inflammatory actions by remodeling cellular?… [] |
| Revista da Associacao Medica Brasileira | A comparison of IL-17 and IL-34 concentrations in the cerebrospinal fluid of patients with acute inflammatory demyelinating neuropathy and chronic?… [] |
| biosciences biotechnology research asia | Dietary Malted Barley Grain Improved Structure and Function of Spinal Cord of Mother Rats fed on a high cholesterol diet. [] |
| ACS Biomaterials Science & Engineering | Tissue-Engineered Decellularized Allografts for Anterior Cruciate Ligament Reconstruction [] |
| Effects of minocycline on neuroinflammation, gut microbiome and hippocampal neurogenesis in rats with Gulf War Illness [] | |
| Minocycline Alleviate A Rat Model Of Gulf War Illness Via Altering Gut Microbiome, Attenuating Neuroinflammation And Enhancing Hippocampal Neurogenesis [] | |
| 中國應(yīng)用生理學(xué)雜志 | 丹參對非酒精性脂肪肝Th17細(xì)胞及相關(guān)細(xì)胞因子的影響* [] |
| Rev Assoc Med Bras | A comparison of IL-17 and IL-34 concentrations in the cerebrospinal fluid of patients with acute inflammatory demyelinating neuropathy and chronic??- [33295414] |
| BEHAVIOURAL BRAIN RESEARCH | Minocycline alleviates Gulf War Illness rats via altering gut microbiome, attenuating neuroinflammation and enhancing hippocampal neurogenesis [34000339] |
| Int J Clin Pract | Serum Galectin?\3 Levels in Patients with Psoriasis [34137138] |
| cells | The Effect of the Clenbuterol?a|?2-Adrenergic Receptor Agonist on the Peripheral Blood Mononuclear Cells Proliferation, Phenotype, Functions, and Reactive Oxygen??- [33920705] |
| Mediators of Inflammation | Sparstolonin B Exerts Therapeutic Effects on Collagen-Induced Arthritis by Inhibiting the NLRP3 Inflammasome and Reducing the Activity of |á1, 3 ?- [] |
| CNS Neurosci Ther | Alterations in the gut microbiome with hemorrhagic transformation in experimental stroke [34591349] |
| PLoS One | Evaluation of the involvement of Th17-cells in the pathogenesis of canine spinal cord injury [34591917] |
| J Periodontol | Cytokine profile in serum and gingival crevicular fluid of children with inflammatory bowel disease: A case‐control study [34730850] |
| Transl Psychiatry | Associations between expression of indoleamine 2, 3-dioxygenase enzyme and inflammatory cytokines in patients with first-episode drug-naive?… [34802039] |
| Viruses | Interleukin-10-Mediated Lymphopenia Caused by Acute Infection with Foot-and-Mouth Disease Virus in Mice [34960627] |
| PLoS One | Th17 cell-mediated immune response in a subpopulation of dogs with idiopathic epilepsy [Pubmed:35025939] |
| Pathogens | Elevated Levels of IL-33, IL-17 and IL-25 Indicate the Progression from Chronicity to Hepatocellular Carcinoma in Hepatitis C Virus Patients [Pubmed:35056005] |
| Biomed Pharmacother | Jiangu granule ameliorated OVX rats bone loss by modulating gut microbiota-SCFAs-Treg/Th17 axis [Pubmed:35453007] |
| npj Biofilms and Microbiomes | The microbiota-gut-kidney axis mediates host osmoregulation in a small desert mammal [Pubmed:35379849] |
| Expert Opinion on Therapeutic Targets | Dapagliflozin mitigates ovalbumin-prompted airway inflammatory-oxidative successions and associated bronchospasm in a rat model of allergic asthma [Pubmed:35549595] |
留言咨詢
