黃體激素(LH)檢測試劑盒(酶聯(lián)免疫吸附試驗(yàn)法)
ELISA Kit for Luteinizing Hormone (LH)
ICSH; Lutropin; Interstitial Cell Stimulating Hormone
- 編號(hào)UCEA441Ra
- 物種Rattus norvegicus (Rat，大鼠) 相同的名稱，不同的物種。
- 實(shí)驗(yàn)方法競爭抑制
- 反應(yīng)時(shí)長2h
- 檢測范圍370.4-30,000pg/mL
- 靈敏度最小可檢測劑量小于等于159.1pg/mL.
- 樣本類型serum, plasma and other biological fluids
- 下載 英文說明書 中文說明書
- 規(guī)格 48T96T 96T*5 96T*10 96T*100
- 價(jià)格 ¥ 1436 ¥ 2052 ¥ 9234 ¥ 17442 ¥ 143640
- 欲了解實(shí)際交易價(jià)格和更多情況，請與當(dāng)?shù)亟?jīng)銷商聯(lián)系!
-
產(chǎn)品包裝(模擬)
-
產(chǎn)品包裝(模擬)
-
實(shí)驗(yàn)結(jié)果圖
-
標(biāo)準(zhǔn)曲線圖
-
通過ISO 9001、ISO 13485質(zhì)量體系認(rèn)證
特異性
本試劑盒用于檢測黃體激素(LH)，經(jīng)檢測與其它相似物質(zhì)無明顯交叉反應(yīng)。
由于受到技術(shù)及樣本來源的限制，不可能完成對所有相關(guān)或相似物質(zhì)交叉反應(yīng)檢測，因此本試劑盒有可能與未經(jīng)檢測的其它物質(zhì)有交叉反應(yīng)。
回收率
分別于定值血清及血漿樣本中加入一定量的黃體激素(LH)（加標(biāo)樣品），重復(fù)測定并計(jì)算其均值，回收率為測定值與理論值的比率。
| 樣本 | 回收率范圍(%) | 平均回收率(%) |
| serum(n=5) | 84-103 | 93 |
| EDTA plasma(n=5) | 91-98 | 94 |
| heparin plasma(n=5) | 89-103 | 99 |
精密度
精密度用樣品測定值的變異系數(shù)CV表示。CV(%) = SD/mean×100
批內(nèi)差：取同批次試劑盒對低、中、高值定值樣本進(jìn)行定量檢測,每份樣本連續(xù)測定20 次，分別計(jì)算不同濃度樣本的平均值及SD值。
批間差：選取3個(gè)不同批次的試劑盒分別對低、中、高值定值樣本進(jìn)行定量測定，每個(gè)樣本使用同一試劑盒重復(fù)測定8次，分別計(jì)算不同濃度樣本的平均值及SD值。
批內(nèi)差： CV<10%
批間差： CV<12%
線性
在定值血清及血漿樣本內(nèi)加入適量的黃體激素(LH)，并倍比稀釋成1:2，1:4，1:8，1:16的待測樣本，線性范圍即為稀釋后樣本中黃體激素(LH)含量的測定值與理論值的比率。
| 樣本 | 1:2 | 1:4 | 1:8 | 1:16 |
| serum(n=5) | 87-104% | 79-91% | 82-91% | 79-98% |
| EDTA plasma(n=5) | 98-105% | 85-99% | 96-103% | 93-101% |
| heparin plasma(n=5) | 90-99% | 87-95% | 98-105% | 80-102% |
穩(wěn)定性
經(jīng)測定，試劑盒在有效期內(nèi)按推薦溫度保存，其活性降低率小于5%。
為減小外部因素對試劑盒破壞前后檢測值的影響，實(shí)驗(yàn)室的環(huán)境條件需盡量保持一致，尤其是實(shí)驗(yàn)室內(nèi)溫度、濕度及溫育條件。其次由同一實(shí)驗(yàn)員來進(jìn)行操作可減少人為誤差。
實(shí)驗(yàn)流程
1. 實(shí)驗(yàn)前標(biāo)準(zhǔn)品、試劑及樣本的準(zhǔn)備；
2. 加樣（標(biāo)準(zhǔn)品及樣本）50µL，
加入50µL檢測液A(臨用前配制);
37°C溫育1小時(shí)。
3. 洗板3次；
4. 加檢測溶液B100µL，37°C孵育30分鐘；
5. 洗板5次；
6. 加TMB底物90µL，37°C孵育10-20分鐘；
7. 加終止液50µL，立即450nm讀數(shù)。
實(shí)驗(yàn)原理
本試劑盒應(yīng)用競爭抑制酶聯(lián)免疫分析法測定標(biāo)本中待測物質(zhì)水平。將黃體激素抗體包被微孔板，制成固相載體，往包被抗體的微孔中同時(shí)加入生物素標(biāo)記的抗原和待測抗原（標(biāo)準(zhǔn)品或樣本），待測抗原與生物素標(biāo)記抗原對特異性抗體進(jìn)行競爭結(jié)合。溫育后經(jīng)洗滌去掉未結(jié)合物，然后加入HRP標(biāo)記的親和素，經(jīng)過溫育和徹底洗滌后加入底物TMB顯色。TMB在過氧化物酶的催化下轉(zhuǎn)化成藍(lán)色，并在酸的作用下轉(zhuǎn)化成最終的黃色。待測標(biāo)本濃度越高，標(biāo)記抗原和抗體的結(jié)合就越受到抑制，顯色愈淺。顯色的深淺與酶量呈正相關(guān)，而與樣品中待測物質(zhì)含量呈負(fù)相關(guān)。用酶標(biāo)儀在450nm波長下測定吸光度（O.D.值），計(jì)算樣品濃度。
贈(zèng)品
相關(guān)產(chǎn)品
| 編號(hào) | 適用物種：Rattus norvegicus (Rat，大鼠) | 應(yīng)用(僅供研究使用，不用于臨床診斷！) |
| URPA441Ra01 | 黃體激素(LH)重組蛋白 | Positive Control; Immunogen; SDS-PAGE; WB. |
| UPAA441Ra01 | 黃體激素(LH)多克隆抗體 | WB; IHC; ICC; IP. |
| ULAA441Ra71 | 黃體激素(LH)多克隆抗體(生物素標(biāo)記) | WB; IHC; ICC. |
| UCEA441Ra | 黃體激素(LH)檢測試劑盒(酶聯(lián)免疫吸附試驗(yàn)法) | Enzyme-linked immunosorbent assay for Antigen Detection. |
| UCCA441Ra | 黃體激素(LH)檢測試劑盒(化學(xué)發(fā)光免疫分析法) | Chemiluminescent immunoassay for Antigen Detection. |
| ULMA441Ra | 黃體激素(LH)等多因子檢測試劑盒(流式熒光發(fā)光法) | FLIA Kit for Antigen Detection. |
參考文獻(xiàn)
| 雜志 | 參考文獻(xiàn) |
| Molecular and Cellular Endocrinology | Effects of genistein and estrogen receptor subtype-specific agonists in ArKO mice following different administration routes. [PubMed: 19686804] |
| International Journal of Clinical and Experimental Medicine | Studies of the pharmacology of 17α-ethynyl-androst-5-ene-3β,7β,17β-triol, a synthetic anti-inflammatory androstene [Ijcem: IJCEM1102004] |
| Biochemical Journal | PPARγ co-activator-1α co-activates steroidogenic factor 1 to stimulate the synthesis of luteinizing hormone and aldosterone [PubMed: 21108604] |
| Molecular and Cellular Endocrinology | Effects of genistein and estrogen receptor subtype-specific agonists in ArKO mice following different administration routes [ScienceDirect: S0303720709004043] |
| Asian Journal of Andrology | Brief maternal exposure of rats to the xenobiotics dibutyl phthalate or diethylstilbestrol alters adult-type Leydig cell development in male offspring [PubMed: 23314658] |
| EXCLI Journal | Protective role of Hibiscus sabdariffa calyx extract against streptozotocin induced sperm damage in diabetic rats [Excli: Source] |
| International Journal of Molecular and Cellular Medicine | Central effects of camphor on GnRH and sexual hormones in male rat [Pubmed: 24551777] |
| Reproductive Toxicology | Inhibitory effects of mitotane on viability and secretory activity in mouse gonadotroph cell lines [Pubmed: 24486453] |
| Urologia internationalis | Effects of Tadalafil on Hemorrhagic Cystitis and Testicular Dysfunction Induced by Cyclophosphamide in Rats [Pubmed: 24051982] |
| Journal of Materials Chemistry B | Nanocomposites of bio-based hyperbranched polyurethane/funtionalized MWCNT as non-immunogenic, osteoconductive, biodegradable and biocompatible scaffolds in bone tissue engineering [Rsc: Source] |
| Electromagnetic Biology and Medicine | The effects of long-term exposure to a 2450 MHz electromagnetic field on growth and pubertal development in female Wistar rats [Informahealthcare: Source] |
| Academic Sciences | Piper longum hexane fraction induces infertility by modulation of inflammatory mediators and gonadotropin insufficiency in female rats [Ijppsjournal: Source] |
| Biochemical and Biophysical Research Communications | Critical function of RA-GEF-2/Rapgef6, a guanine nucleotide exchange factor for Rap1, in mouse spermatogenesis [ScienceDirect: S0006291X14001843] |
| Reproductive Toxicology | Carbamazepine-exposure during gestation and lactation affects pubertal onset and spermatic parameters in male pubertal offspring [Pubmed:24126190] |
| Mol Cell Endocrinol. | Quercetin supplementation restores testicular function and augments germ cell survival in the estrogenized rats [Pubmed:24316378] |
| Andrologia | Protective effects of Eugenia jambolana extract versus N-acetyl cysteine against cisplatin-induced damage in rat testis [Pubmed:24576220] |
| Environmental Research | Perfluorooctane sulfonate effects on the reproductive axis in adult male rats [Pubmed:25171141] |
| Genet Mol Biol. | Targeted disruption of the mouse testis-enriched gene Znf230 does not affect spermatogenesis or fertility [Pubmed:25505846] |
| Turkish Journal of Urology | Apoptosis in testicular tissue of rats after vasectomy: evaluation of eNOS, iNOS immunoreactivities and the effects of ozone therapy [Turkishjournalofurology:Source] |
| Andrologia | Protective effects of Eugenia jambolana extract versus N‐acetyl cysteine against cisplatin‐induced damage in rat testis [PubMed: 24576220] |
| European Journal of Pharmacology | The effect of R-(-)-deprenyl administration on reproductive parameters of rat males [PubMed: 25725114] |
| Andrologia | Dose‐and time‐dependent effects of Garcinia kola seed extract on sexual behaviour and reproductive parameters in male Wistar rats [PubMed: 26123866] |
| Reprod Toxicol | Simvastatin decreases steroid production in the H295R cell line and decreases steroids and FSH in female rats [PubMed: 26476359] |
| Biology of Reproduction | Maternal High-Fat Diet Induced Loss of Fetal Oocytes Is Associated with Compromised Follicle Growth in Adult Rat Offspring [Pubmed:26962114] |
| Andrologia | Dose- and time-dependent?effects?of?Garcinia kola?seed?extract?on?sexual behaviour?and?reproductiveparameters?in?male Wistar rats. [Pubmed:26123866] |
| Int J Mol Sci. | Overexpression of PRL7D1 in Leydig Cells Causes Male Reproductive Dysfunction in Mice [Pubmed:26771609] |
| PLoS Genet. | A Jacob/Nsmf Gene Knockout Results in Hippocampal Dysplasia and Impaired BDNF Signaling in Dendritogenesis [Pubmed:26977770] |
| Scientific Reports | Molecular characterization of insulin resistance and glycolytic metabolism in the rat uterus [pubmed:27461373] |
| 33 | Metformin Ameliorates Uterine Defects in a Rat Model of Polycystic Ovary Syndrome. [pubmed:28336389] |
| environmental pollution | Environmental exposure to polycyclic aromatic hydrocarbons (PAHs): The correlation with and impact on reproductive hormones in umbilical cord serum. [pubmed:27838061] |
| Toxicological?Sciences | Exposure of pregnant mice to perfluorobutanesulfonate causes hypothyroxinemia and developmental abnormalities in female offspring [pubmed:27803384] |
| Andrologia | Effects?of?treatment?with?Hypoxis?hemerocallidea?extract?on?sexual?behaviour?and?reproductiveparameters?in?male?rats. [pubmed:28000943] |
| Biology?of?Reproduction | Maternal High-Fat Diet-Induced Loss of Fetal Oocytes Is Associated with Compromised Follicle Growth in Adult Rat Offspring. [pubmed:26962114] |
| J Endocrinol. | Impact of uteroplacental insufficiency on postnatal rat male gonad. [pubmed:27885054] |
| Int J Pharm. | New gonadotropin-releasing hormone glycolipids with direct antiproliferative activity and gonadotropin-releasing potency. [pubmed:28232269] |
| Environmental Science & Technology | Pyrethroid Insecticide Cypermethrin Accelerates Pubertal Onset in Male Mice via Disrupting Hypothalamic-Pituitary-Gonadal Axis [pubmed:28731686] |
| Journal of Medicinal Chemistry | Development of New Gonadotropin-Releasing Hormone-Modified Dendrimer Platforms with Direct Antiproliferative and Gonadotropin Releasing Activity [pubmed:28958147] |
| Society of Toxicology | Pyrethroid Insecticide Cypermethrin Modulates Gonadotropin Synthesis via Calcium Homeostasis and ERK1/2 Signaling in LbT2 Mouse Pituitary Cells [pubmed:29149324] |
| Reproductive Toxicology | Vitamin D deficiency impairs testicular development and spermatogenesis in mice [pubmed:28655646] |
| The Journal of Biological Chemistry. | Lysophosphatidic acid acyltransferase 3 tunes the membrane status of germ cells by incorporating docosahexaenoic acid during spermatogenesis. [pubmed:28578315] |
| The Journal of Toxicological Sciences | High sensitivity of testicular function to titanium nanoparticles [pubmed:28496042] |
| Journal of Orthopaedic Research | The Combination of icariin and constrained dynamic loading stimulation attenuates bone loss in ovariectomy‐induced osteoporotic mice [pubmed:29058779] |
| Anticancer Research | Transdermal Delivery of Luteinizing Hormone-releasing Hormone with Chitosan Microneedles: A Promising Tool for Androgen Deprivation Therapy [pubmed:29187457] |
| DARU Journal of Pharmaceutical Sciences | Beneficial effects of Heqi san on rat model of polycystic ovary syndrome through the PI3K/AKT pathway [pubmed:29020999] |
| Reproduction. | Effect of controlled ovarian hyperstimulation on puberty and estrus in mice offspring [pubmed:28687593] |
| Archives?of?Toxicology | Atorvastatin decreases steroid production in H295R cells and in major endocrine tissues of male rats [Pubmed:29574565] |
| FASEB?Journal | Vasorin: a newly identified regulator of ovarian folliculogenesis [Pubmed:29259033] |
| Stem?Cell?Research?&?Therapy | The role of mesenchymal stem cells in chemotherapy-induced gonadotoxicity [Pubmed:30021657] |
| Environmental Pollution | Prenatal exposure to polychlorinated biphenyl and umbilical cord hormones and birth outcomes in an island population [Pubmed:29525625] |
| Toxicological?Sciences | Impact of Perfluorooctane Sulfonate on Reproductive Ability of Female Mice through Suppression of Estrogen Receptor a-Activated Kisspeptin Neurons [10.1093:toxsci] |
| Frontiers in Molecular Neuroscience | Impact of Triclosan on Female Reproduction through Reducing Thyroid Hormones to Suppress Hypothalamic Kisspeptin Neurons in Mice [Pubmed:29403355] |
| Biology of Reproduction | Maternal nutrient restriction impairs young adult offspring ovarian signaling resulting in reproductive dysfunction and follicle loss [Pubmed:29351580] |
| Journal of?Ovarian?Research | DHEA-induced ovarian hyperfibrosis is mediated by TGF-β signaling pathway [Pubmed:29321035] |
| Medical Science Monitor | Eicosapentaenoic Acid Improves Polycystic Ovary Syndrome in Rats via Sterol Regulatory Element-Binding Protein 1 (SREBP-1)/Toll-Like Receptor 4 (TLR4)?… [Pubmed:29627845] |
| Human & Experimental Toxicology | Palladium nanoparticle effects on endocrine reproductive system of female rats [Pubmed:29405765] |
| A Pex13 knockout in germ cells induces a spermatogenic arrest [volltexte:2018/8921/] | |
| Biomedicine & Pharmacotherapy | Crocetin attenuates DHT-induced polycystic ovary syndrome in mice via revising kisspeptin neurons [Doi: 10.1016/j.biopha.2018.08.135] |
| 第三軍醫(yī)大學(xué)學(xué)報(bào) | 基因敲除損傷小鼠睪丸睪酮產(chǎn)生與生精功能 [] |
| Biological?Rhythm?Research | Effects of feeding frequency during lactation in primiparous sows on reproduction performance and diurnal rhythm of endocrine profiles at the weaning-to-ovulation?… [] |
| Stem?Cells?Translational?Medicine | Protective Effects of Uncultured Adipose‐Derived Stromal Vascular Fraction on Testicular Injury Induced by Torsion‐Detorsion in Rats [Pubmed: 30569668] |
| Journal of Endocrinology | Uterine progesterone signaling is a target for metformin therapy in polycystic ovary syndrome [Pubmed: 29535146] |
| Experimental and Therapeutic Medicine | Tanshinone IIA attenuates estradiol?induced polycystic ovarian syndrome in mice by ameliorating FSHR expression in the ovary [] |
| Theriogenology | Organic Zn and Cu interaction impact on sexual behaviour, semen characteristics, hormones and spermatozoal gene expression in bucks (Capra hircus) [] |
| International Journal of Reproductive Biomedicine | The therapeutic potential of amifostine on cyclophosphamide-induced testicular dysfunction in rats: An experimental study. [] |
| Andrologia | Sperm parameters quality and reproductive effects of methanolic extract of Alchornea cordifolia leaves on senescent male rats [Pubmed: 31353623] |
| Journal of Ovarian Research | Baicalin ameliorates polycystic ovary syndrome through AMP-activated protein kinase [Pubmed: 31722718] |
| Chemosphere | Exposure of adult mice to perfluorobutanesulfonate impacts ovarian functions through hypothyroxinemia leading to down-regulation of Akt-mTOR signaling [Pubmed: 31809938] |
| Medicina | Effect of DHT-Induced Hyperandrogenism on the Pro-Inflammatory Cytokines in a Rat Model of Polycystic Ovary Morphology [Pubmed: 32120970] |
| Chemosphere | Prenatal exposure of female mice to per?uorononanoic acid delays pubertal activation of the reproductive endocrine axis through enhanced hepatic FGF21?… [Pubmed: 33131727] |
| BIOLOGICAL RESEARCH | Novel mechanisms underlying anti-polycystic ovary like syndrome effects of electroacupuncture in rats: suppressing SREBP1 to mitigate insulin resistance?… [Pubmed: 33109277] |
| ARCHIVES OF GYNECOLOGY AND OBSTETRICS | MiRNAs expression profiling of rat ovaries displaying PCOS with insulin resistance [Pubmed: 32757043] |
| Mol Med | Electroacupuncture alleviates polycystic ovary syndrome-like symptoms through improving insulin resistance, mitochondrial dysfunction, and endoplasmic?… [Pubmed: 32698821] |
| Antioxidants (Basel) | Composition of Royal Jelly (RJ) and Its Anti-Androgenic Effect on Reproductive Parameters in a Polycystic Ovarian Syndrome (PCOS) Animal Model [Pubmed: 32517356] |
| chemosphere | N, N-Dimethylacetamide, an FDA approved excipient, acts post-meiotically to impair spermatogenesis and cause infertility in rats [Pubmed: 32447106] |
| Neurotoxicology | Chronic exposure to low dose of bisphenol A causes follicular atresia by inhibiting kisspeptin neurons in anteroventral periventricular nucleus in female mice [Pubmed: 32407858] |
| FASEB JOURNAL | Acyl-CoA synthetase 6 regulates long-chain polyunsaturated fatty acid composition of membrane phospholipids in spermatids and supports normal spermatogenic?… [Pubmed: 31648559] |
| BioMed Research International | Pathophysiological Changes in Female Rats with Estrous Cycle Disorder Induced by Long-Term Heat Stress [Pubmed: 32685488] |
| ACS Applied Bio Materials | Evaluation for adverse effects of InP/ZnS quantum dots on the in vitro cultured oocytes of mice [] |
| Iranian Journal of Basic Medical Sciences | Therapeutic effects of organic zinc on reproductive hormones, insulin resistance and mTOR expression as a novel component in a rat model of PCOS [] |
| Medical Journal of Cairo University | Hemin Ameliorated Letrozole-Induced Polycystic Ovary Syndrome: A Forward Step for Better Management [] |
| JOURNAL OF ETHNOPHARMACOLOGY | Zihuai recipe alleviates cyclophosphamide-induced diminished ovarian reserve via suppressing PI3K/AKT-mediated apoptosis [33422655] |
| advanced biomedical research | The Effects of Zinc Methionine on Reproductive and Thyroid Hormones in Rats with Polycystic Ovarian Syndrome [33457340] |
| Endocrinology | Diabetes Type 1 Negatively Influences Leydig Cell Function in Rats, Which is Partially Reversible By Insulin Treatment [33507237] |
| JOURNAL OF ETHNOPHARMACOLOGY | BaZiBuShen alleviates altered testicular morphology and spermatogenesis and modulates Sirt6/P53 and Sirt6/NF-|êB pathways in aging mice induced by D-galactose [33508368] |
| Toxicol Appl Pharmacol | Exposure to the bromodomain inhibitor N-methyl pyrrolidone blocks spermatogenesis in a hormonal and non-hormonal fashion [33965371] |
| Nutr Metab | Effects of increasing intake of soybean oil on synthesis of testosterone in Leydig cells [34039393] |
| plants | Tribulus terrestris Efficacy and Safety Concerns in Diabetes and Erectile Dysfunction, Assessed in an Experimental Model [33920217] |
| researchsquare | Lipoamino Acid-Modified GnRH Analogs with Receptor-Mediated Antiproliferative Activity in Prostate and Ovarian Cancer Cells [] |
| Scientific reports | Regulation of Uterine Function During Estrous Cycle, Anestrus Phase and Pregnancy by Steroids in Red Deer (Cervus elaphus L.) [] |
| Chronic Exposure to Perfluorohexane Sulfonate Leads to a Reproduction Deficit by Suppressing Hypothalamic Kisspeptin Expression in Mice [] | |
| JOURNAL OF INHERITED METABOLIC DISEASE | Human and animal fertility studies in cystinosis reveal signs for obstructive azoospermia, a disruptive blood‐testis barrier and a subtherapeutic effect of cysteamine in?… [34494673] |
| Free Radical Biology and Medicine | Cadmium attenuates testosterone synthesis by promoting ferroptosis and blocking autophagosome-lysosome fusion [34610361] |
| Microbiome | Polyamine metabolism links gut microbiota and testicular dysfunction [34758869] |
| J Virol | Zika virus infection in the ovary induces continuously elevated progesterone level and compromises conception in interferon α/β receptor-deficient mice [34730391] |
| Cell Death Dis | METTL3-mediated mRNA N6-methyladenosine is required for oocyte and follicle development in mice [34689175] |
| Theriogenology | Superior effect of broccoli methanolic extract on control of oxidative damage of sperm cryopreservation and reproductive performance in rats: A comparison with?… [Pubmed:35063921] |
| Nutrients | Yogurt Enriched with Inulin Ameliorated Reproductive Functions and Regulated Gut Microbiota in Dehydroepiandrosterone-Induced Polycystic Ovary Syndrome Mice [Pubmed:35057459] |
| Sci Total Environ | Exposure to multiple pyrethroid insecticides affects ovarian follicular development via modifying microRNA expression [Pubmed:35276145] |
| Ecotoxicology and Environmental Safety | Constitutional delay of growth and puberty in female mice is induced by circadian rhythm disruption in utero [Pubmed:35679725] |
| BMC Veterinary Research | A promising animal model for Polycystic Ovary Syndrome: Borderline Hypertensive Rats [] |
留言咨詢
