S100鈣結合蛋白B(S100B)檢測試劑盒(酶聯免疫吸附試驗法)
ELISA Kit for S100 Calcium Binding Protein B (S100B)
S100-B; NEF; S100Beta; S-100 protein subunit beta
- 編號USEA567Rb
- 物種Oryctolagus cuniculus (Rabbit，兔) 相同的名稱，不同的物種。
- 實驗方法雙抗夾心
- 反應時長3h
- 檢測范圍15.6-1,000pg/mL
- 靈敏度最小可檢測劑量小于等于6.3pg/mL.
- 樣本類型Serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
- 下載 英文說明書 中文說明書
- 規(guī)格 48T96T 96T*5 96T*10 96T*100
- 價格 ¥ 1436 ¥ 2052 ¥ 9234 ¥ 17442 ¥ 143640
- 欲了解實際交易價格和更多情況，請與當地經銷商聯系!
-
產品包裝(模擬)
-
產品包裝(模擬)
-
實驗結果圖
-
標準曲線圖
-
通過ISO 9001、ISO 13485質量體系認證
特異性
本試劑盒用于檢測S100鈣結合蛋白B(S100B)，經檢測與其它相似物質無明顯交叉反應。
由于受到技術及樣本來源的限制，不可能完成對所有相關或相似物質交叉反應檢測，因此本試劑盒有可能與未經檢測的其它物質有交叉反應。
回收率
分別于定值血清及血漿樣本中加入一定量的S100鈣結合蛋白B(S100B)（加標樣品），重復測定并計算其均值，回收率為測定值與理論值的比率。
| 樣本 | 回收率范圍(%) | 平均回收率(%) |
| serum(n=5) | 98-105 | 102 |
| EDTA plasma(n=5) | 86-98 | 94 |
| heparin plasma(n=5) | 89-98 | 92 |
精密度
精密度用樣品測定值的變異系數CV表示。CV(%) = SD/mean×100
批內差：取同批次試劑盒對低、中、高值定值樣本進行定量檢測,每份樣本連續(xù)測定20 次，分別計算不同濃度樣本的平均值及SD值。
批間差：選取3個不同批次的試劑盒分別對低、中、高值定值樣本進行定量測定，每個樣本使用同一試劑盒重復測定8次，分別計算不同濃度樣本的平均值及SD值。
批內差： CV<10%
批間差： CV<12%
線性
在定值血清及血漿樣本內加入適量的S100鈣結合蛋白B(S100B)，并倍比稀釋成1:2，1:4，1:8，1:16的待測樣本，線性范圍即為稀釋后樣本中S100鈣結合蛋白B(S100B)含量的測定值與理論值的比率。
| 樣本 | 1:2 | 1:4 | 1:8 | 1:16 |
| serum(n=5) | 78-97% | 92-99% | 87-96% | 96-105% |
| EDTA plasma(n=5) | 93-105% | 94-103% | 78-104% | 86-97% |
| heparin plasma(n=5) | 88-104% | 96-103% | 78-99% | 79-89% |
穩(wěn)定性
經測定，試劑盒在有效期內按推薦溫度保存，其活性降低率小于5%。
為減小外部因素對試劑盒破壞前后檢測值的影響，實驗室的環(huán)境條件需盡量保持一致，尤其是實驗室內溫度、濕度及溫育條件。其次由同一實驗員來進行操作可減少人為誤差。
實驗流程
1. 實驗前標準品、試劑及樣本的準備；
2. 加樣（標準品及樣本）100µL，37°C孵育1小時；
3. 吸棄，加檢測溶液A100µL，37°C孵育1小時；
4. 洗板3次；
5. 加檢測溶液B100µL，37°C孵育30分鐘；
6. 洗板5次；
7. 加TMB底物90µL，37°C孵育10-20分鐘；
8. 加終止液50µL，立即450nm讀數。
實驗原理
將S100鈣結合蛋白B(S100B)抗體包被于96孔微孔板中，制成固相載體，向微孔中分別加入標準品或標本，其中的S100鈣結合蛋白B(S100B)與連接于固相載體上的抗體結合，然后加入生物素化的S100鈣結合蛋白B(S100B)抗體，將未結合的生物素化抗體洗凈后，加入HRP標記的親和素，再次徹底洗滌后加入TMB底物顯色。TMB在過氧化物酶的催化下轉化成藍色，并在酸的作用下轉化成最終的黃色。顏色的深淺和樣品中的S100鈣結合蛋白B(S100B)呈正相關。用酶標儀在450nm波長下測定吸光度(O.D.值)，計算樣品濃度。
增值服務
相關產品
| 編號 | 適用物種：Oryctolagus cuniculus (Rabbit，兔) | 應用(僅供研究使用，不用于臨床診斷！) |
| URPA567Rb01 | S100鈣結合蛋白B(S100B)重組蛋白 | Positive Control; Immunogen; SDS-PAGE; WB. |
| UPAA567Rb01 | S100鈣結合蛋白B(S100B)多克隆抗體 | IHC |
| UPAA567Rb52 | S100鈣結合蛋白B(S100B)多克隆抗體 | WB; IHC; ICC; IP. |
| UPAA567Rb51 | S100鈣結合蛋白B(S100B)多克隆抗體 | IHC |
| ULAA567Rb71 | S100鈣結合蛋白B(S100B)多克隆抗體(生物素標記) | IHC |
| ULAA567Rb81 | S100鈣結合蛋白B(S100B)多克隆抗體(異硫氰酸熒光素標記) | WB; IHC; ICC; IF. |
| USEA567Rb | S100鈣結合蛋白B(S100B)檢測試劑盒(酶聯免疫吸附試驗法) | Enzyme-linked immunosorbent assay for Antigen Detection. |
| ULMA567Rb | S100鈣結合蛋白B(S100B)等多因子檢測試劑盒(流式熒光發(fā)光法) | FLIA Kit for Antigen Detection. |
| UKSA567Rb01 | S100鈣結合蛋白B(S100B)檢測試劑盒DIY材料(酶聯免疫吸附試驗法) | 自己動手制作ELISA試劑盒的主要材料 |
參考文獻
| 雜志 | 參考文獻 |
| Epilepsia | Protective effects of naloxone in two-hit seizure model [PubMed: 19674055] |
| Journal of Neurotrauma | The Anti-Inflammatory and Neuroprotective Effects of Ghrelin in Subarachnoid Hemorrhage-Induced Oxidative Brain Damage in Rats [Liebert: 20091210] |
| Food and Chemical Toxicology | Sub-acute effects of diazinon on biochemical indices and specific biomarkers in rats: Protective effects of crocin and safranal [PubMed: 20637253] |
| 20 | Melatonin treatment protects against spinal cord injury induced functional and biochemical changes in rat urinary bladder [Wiley: source] |
| Neuroscience Letters | A preliminary study of the effects of ulinastatin on early postoperative cognition function in patients undergoing abdominal surgery [Pubmed: 23422155] |
| Genes Cells | Induction of receptor for advanced glycation end products by insufficient leptin action triggers pancreatic β-cell failure in type 2 diabetes. [Pubmed: 23410183] |
| J Craniofac Surg. | The Experiment Research of Neuroprotection of Hypoperfusion Postconditioning on Cerebral Ischemia [Pubmed:25006960] |
| Int J Mol Sci | The effect of electroporation of a lyotroic liquid crystal Genistein-based formulation in the recovery of murine melanoma lesions [PubMed: 26184156] |
| Mol Neurobiol | The Severity of Cecal Ligature and Puncture-Induced Sepsis Correlates with the Degree of Encephalopathy, but the Sepsis Does Not Lead to Acute Activation of Spleen Lymphocytes in Mice [PubMed: 26081155] |
| Pediatria i Medycyna Rodzinna | St??enie bia?ka S100B w surowicy krwi u m?odzie?y z omdleniami [yadda:60e5630d0ac1] |
| 中國臨床藥理學與治療學 | 右美托咪定對腦膜瘤切除術患者血清S100β 蛋白及神經元特異性烯醇化酶的影響 [article:9794] |
| Eur J Pharmacol.? | Development of an experimental rat model of hyperammonemic encephalopathy and evaluation of the effects of rifaximin [Pubmed:26980242] |
| Journal For?Neurovirology | Soluble?TLR2?and?4?concentrations?in?cerebrospinal?fluid?in?HIV/SIV-relatedneuropathological?conditions. [pubmed:27882497] |
| Neurourol?Urodyn | Functional and structural changes of the urinary bladder following spinal cord injury; treatment with alpha lipoic acid [pubmed:27490041] |
| cellular?physiology and biochemistry | Hydrogen-Rich Saline Attenuates Brain Injury Induced by Cardiopulmonary Bypass and Inhibits Microvascular Endothelial Cell Apoptosis Via the PI3K/Akt/GSK3β … [pubmed:29040978] |
| Biomedicine & pharmacotherapy | Berberine ameliorates intrahippocampal kainate-induced status epilepticus and consequent epileptogenic process in the rat: Underlying mechanisms [pubmed:28061403] |
| Cellular Physiology and Biochemistry | Activated Α7nachr Improves Postoperative Cognitive Dysfunction and Intestinal Injury Induced by Cardiopulmonary Bypass in Rats: Inhibition of the Proinflammatory?… [Pubmed:29672286] |
| Brain?Research | Intra-arterial human urinary kallidinogenase alleviates brain injury in rats with permanent middle cerebral artery occlusion through PI3K/AKT/FoxO1 signaling pathway [Pubmed:29510144] |
| Neurochemical Research | Protective Effects of Cornel Iridoid Glycoside in Rats After Traumatic Brain Injury [Pubmed:29492766] |
| Biochemical and Biophysical?Research?Communications | C6 glioma-conditioned medium induces malignant transformation of mesenchymal stem cells: Possible role of S100B/RAGE pathway [Pubmed:29050939] |
| Journal of the Neurological?Sciences | S100B, NSE and MMP-9 fail to predict neurologic outcome while elevated S100B associates with milder initial clinical presentation after aneurysmal subarachnoid?… [Pubmed:29801873] |
| Biochemistry and?Cell?Biology | Ectopic expression of Miro 1 ameliorates seizures and inhibits hippocampal neurodegeneration in a mouse model of pilocarpine epilepsy [Pubmed:29365285] |
| S100B as a potential biomarker for concussion in male and female collegiate rugby athletes [:] | |
| Indian?Journal?of?Psychiatry | Serum S100B levels in patients with depression [] |
| Evidence-Based Complementary and Alternative Medicine | Naomaitai Ameliorated Brain Damage in Rats with Vascular Dementia by PI3K/PDK1/AKT Signaling Pathway [] |
| Brain?Sciences | Extracellular S100β Disrupts Bergman Glia Morphology and Synaptic Transmission in Cerebellar Purkinje Cells [Pubmed: 31013844] |
| Medicina | The Administration of the New Pyrimidine Derivative—4-{2-[2-(3, 4-Dimethoxyphenyl)-Vinyl]-6-Ethyl-4-Oxo-5-Phenyl-4H-Pyrimidine-1-Il} Benzsulfamide Restores the?… [] |
| ACTA NEUROBIOLOGIAE EXPERIMENTALIS | Neuroprotective effect of poly (lactic-co-glycolic acid) nanoparticle-bound brain-derived neurotrophic factor in a permanent middle cerebral artery occlusion?… [Pubmed: 32214270] |
| Stem Cells International | Exosomes of Antler Mesenchymal Stem Cells Improve Postoperative Cognitive Dysfunction in Cardiopulmonary Bypass Rats through Inhibiting the TLR2?… [Pubmed: 32300366] |
| Neurocritical Care | Biomarkers of Neurological Outcome After Aneurysmal Subarachnoid Hemorrhage as Early Predictors at Discharge from an Intensive Care Unit [Pubmed: 32978732] |
| European Review for Medical and Pharmacological Sciences | Neuroprotective effect of CTRP3 overexpression against sevoflurane anesthesia-induced cognitive dysfunction in aged rats through activating AMPK/SIRT1 and … [Pubmed: 32432773] |
| ИЗУЧЕНИЕ ЦЕРЕБРОПРОТЕКТОРНОГО ДЕЙСТВИЯ ПРЕПАРАТОВ РАЗЛИЧНЫХ ФАРМАКОЛОГИЧЕСКИХ ГРУПП В УСЛОВИЯХ?… [] | |
| Scientific Reports | Potential biomarkers of childhood brain tumor identified by proteomics of cerebrospinal fluid from extraventricular drainage (EVD) [33469081] |
| Crit Care | Serum biomarkers and cerebral autoregulation as early warnings of delayed cerebral ischemia risk in patients after aneurysmal subarachnoid haemorrhage [27885969] |
| Transl Psychiatry | Increased GFAP concentrations in the cerebrospinal fluid of patients with unipolar depression [34021122] |
| Biochimie | Neuroprotective effect of 6-hydroxy-2, 2, 4-trimethyl-1, 2-dihydroquinoline mediated via regulation of antioxidant system and inhibition of inflammation and apoptosis in??- [33964368] |
| Int J Neuropsychopharmacol | High S100B levels predict antidepressant response in patients with major depression even when considering inflammatory and metabolic markers [Pubmed:35176771] |
| Metab Brain Dis | 1-benzoyl-6-hydroxy-2, 2, 4-trimethyl-1, 2-dihydroquinoline exerts a neuroprotective effect and normalises redox homeostasis in a rat model of cerebral ischemia?… [Pubmed:35201554] |
| PLoS One | Neuroprotective effect of sodium alginate against chromium-induced brain damage in rats [Pubmed:35421180] |
| International Journal of Molecular Sciences | Sepsis Encephalopathy Is Partly Mediated by miR370-3p-Induced Mitochondrial Injury but Attenuated by BAM15 in Cecal Ligation and Puncture Sepsis Male Mice [Pubmed:35628259] |
| Authorea | THE EVALUATION OF MEDULLA SPINALIS PERFUSION AT MODERATE HYPOTHERMIA WITH PATIENTS WHO UNDERGO ANTEGRADE SELECTIVE CEREBRAL?… [] |
| EPIDEMIOLOGIA, DETERMINANTELE ?I PREDIC?IA ACCIDENTULUI VASCULAR CEREBRAL ISCHEMIC LA COPII [] |
留言咨詢
