轉(zhuǎn)化生長因子β3(TGFb3)檢測試劑盒(酶聯(lián)免疫吸附試驗法)
ELISA Kit for Transforming Growth Factor Beta 3 (TGFb3)
TGF-B3; LAP; Latency-associated peptide
- 編號USEB949Hu
- 物種Homo sapiens (Human，人) 相同的名稱，不同的物種。
- 實驗方法雙抗夾心
- 反應(yīng)時長3h
- 檢測范圍15.6-1,000pg/mL
- 靈敏度最小可檢測劑量小于等于5.7pg/mL.
- 樣本類型serum, plasma, tissue homogenates, cell culture supernates and other biological fluids
- 下載 英文說明書 中文說明書
- 規(guī)格 48T96T 96T*5 96T*10 96T*100
- 價格 ¥ 1397 ¥ 1995 ¥ 8978 ¥ 16958 ¥ 139650
- 欲了解實際交易價格和更多情況，請與當(dāng)?shù)亟?jīng)銷商聯(lián)系!
-
產(chǎn)品包裝(模擬)
-
產(chǎn)品包裝(模擬)
-
實驗結(jié)果圖
-
標(biāo)準(zhǔn)曲線圖
-
通過ISO 9001、ISO 13485質(zhì)量體系認(rèn)證
特異性
本試劑盒用于檢測轉(zhuǎn)化生長因子β3(TGFb3)，經(jīng)檢測與其它相似物質(zhì)無明顯交叉反應(yīng)。
由于受到技術(shù)及樣本來源的限制，不可能完成對所有相關(guān)或相似物質(zhì)交叉反應(yīng)檢測，因此本試劑盒有可能與未經(jīng)檢測的其它物質(zhì)有交叉反應(yīng)。
回收率
分別于定值血清及血漿樣本中加入一定量的轉(zhuǎn)化生長因子β3(TGFb3)（加標(biāo)樣品），重復(fù)測定并計算其均值，回收率為測定值與理論值的比率。
| 樣本 | 回收率范圍(%) | 平均回收率(%) |
| serum(n=5) | 78-95 | 82 |
| EDTA plasma(n=5) | 80-96 | 90 |
| heparin plasma(n=5) | 81-98 | 85 |
精密度
精密度用樣品測定值的變異系數(shù)CV表示。CV(%) = SD/mean×100
批內(nèi)差：取同批次試劑盒對低、中、高值定值樣本進(jìn)行定量檢測,每份樣本連續(xù)測定20 次，分別計算不同濃度樣本的平均值及SD值。
批間差：選取3個不同批次的試劑盒分別對低、中、高值定值樣本進(jìn)行定量測定，每個樣本使用同一試劑盒重復(fù)測定8次，分別計算不同濃度樣本的平均值及SD值。
批內(nèi)差： CV<10%
批間差： CV<12%
線性
在定值血清及血漿樣本內(nèi)加入適量的轉(zhuǎn)化生長因子β3(TGFb3)，并倍比稀釋成1:2，1:4，1:8，1:16的待測樣本，線性范圍即為稀釋后樣本中轉(zhuǎn)化生長因子β3(TGFb3)含量的測定值與理論值的比率。
| 樣本 | 1:2 | 1:4 | 1:8 | 1:16 |
| serum(n=5) | 93-101% | 95-102% | 99-105% | 98-105% |
| EDTA plasma(n=5) | 90-101% | 94-102% | 81-91% | 87-95% |
| heparin plasma(n=5) | 95-105% | 83-95% | 97-105% | 86-101% |
穩(wěn)定性
經(jīng)測定，試劑盒在有效期內(nèi)按推薦溫度保存，其活性降低率小于5%。
為減小外部因素對試劑盒破壞前后檢測值的影響，實驗室的環(huán)境條件需盡量保持一致，尤其是實驗室內(nèi)溫度、濕度及溫育條件。其次由同一實驗員來進(jìn)行操作可減少人為誤差。
實驗流程
1. 實驗前標(biāo)準(zhǔn)品、試劑及樣本的準(zhǔn)備；
2. 加樣（標(biāo)準(zhǔn)品及樣本）100µL，37°C孵育1小時；
3. 吸棄，加檢測溶液A100µL，37°C孵育1小時；
4. 洗板3次；
5. 加檢測溶液B100µL，37°C孵育30分鐘；
6. 洗板5次；
7. 加TMB底物90µL，37°C孵育10-20分鐘；
8. 加終止液50µL，立即450nm讀數(shù)。
實驗原理
將轉(zhuǎn)化生長因子β3(TGFb3)抗體包被于96孔微孔板中，制成固相載體，向微孔中分別加入標(biāo)準(zhǔn)品或標(biāo)本，其中的轉(zhuǎn)化生長因子β3(TGFb3)與連接于固相載體上的抗體結(jié)合，然后加入生物素化的轉(zhuǎn)化生長因子β3(TGFb3)抗體，將未結(jié)合的生物素化抗體洗凈后，加入HRP標(biāo)記的親和素，再次徹底洗滌后加入TMB底物顯色。TMB在過氧化物酶的催化下轉(zhuǎn)化成藍(lán)色，并在酸的作用下轉(zhuǎn)化成最終的黃色。顏色的深淺和樣品中的轉(zhuǎn)化生長因子β3(TGFb3)呈正相關(guān)。用酶標(biāo)儀在450nm波長下測定吸光度(O.D.值)，計算樣品濃度。
增值服務(wù)
相關(guān)產(chǎn)品
| 編號 | 適用物種：Homo sapiens (Human，人) | 應(yīng)用(僅供研究使用，不用于臨床診斷！) |
| URPB949Hu02 | 轉(zhuǎn)化生長因子β3(TGFb3)重組蛋白 | Positive Control; Immunogen; SDS-PAGE; WB. |
| UAPB949Hu02 | 轉(zhuǎn)化生長因子β3(TGFb3)活性蛋白 | Cell?culture;?Activity?Assays. |
| URPB949Hu01 | 轉(zhuǎn)化生長因子β3(TGFb3)重組蛋白 | Positive Control; Immunogen; SDS-PAGE; WB. |
| UEPB949Hu61 | 轉(zhuǎn)化生長因子β3(TGFb3)真核蛋白 | Positive Control; Immunogen; SDS-PAGE; WB. |
| URPB949Hu03 | 轉(zhuǎn)化生長因子β3(TGFb3)重組蛋白 | Positive Control; Immunogen; SDS-PAGE; WB. |
| UAPB949Hu01 | 轉(zhuǎn)化生長因子β3(TGFb3)活性蛋白 | Cell?culture;?Activity?Assays. |
| UPAB949Hu01 | 轉(zhuǎn)化生長因子β3(TGFb3)多克隆抗體 | IHC |
| UPAB949Hu02 | 轉(zhuǎn)化生長因子β3(TGFb3)多克隆抗體 | IHC; ICC/IF |
| ULAB949Hu71 | 轉(zhuǎn)化生長因子β3(TGFb3)多克隆抗體(生物素標(biāo)記) | WB; IHC; ICC. |
| UMAB949Hu23 | 轉(zhuǎn)化生長因子β3(TGFb3)單克隆抗體 | IHC; ICC/IF |
| URAB949Hu21 | 轉(zhuǎn)化生長因子β3(TGFb3)重組抗體 | WB; IF; ICC; IHC; IP; FCM. |
| UMAB949Hu22 | 轉(zhuǎn)化生長因子β3(TGFb3)單克隆抗體 | WB |
| UMAB949Hu24 | 轉(zhuǎn)化生長因子β3(TGFb3)單克隆抗體 | WB; IHC; ICC/IF |
| UMAB949Hu26 | 轉(zhuǎn)化生長因子β3(TGFb3)單克隆抗體 | ICC/IF |
| UMAB949Hu25 | 轉(zhuǎn)化生長因子β3(TGFb3)單克隆抗體 | ICC/IF |
| UMAB949Hu21 | 轉(zhuǎn)化生長因子β3(TGFb3)單克隆抗體 | WB; IHC; ICC/IF |
| USEB949Hu | 轉(zhuǎn)化生長因子β3(TGFb3)檢測試劑盒(酶聯(lián)免疫吸附試驗法) | Enzyme-linked immunosorbent assay for Antigen Detection. |
| USCB949Hu | 轉(zhuǎn)化生長因子β3(TGFb3)檢測試劑盒(化學(xué)發(fā)光免疫分析法) | Chemiluminescent immunoassay for Antigen Detection. |
| ULMB949Hu | 轉(zhuǎn)化生長因子β3(TGFb3)等多因子檢測試劑盒(流式熒光發(fā)光法) | FLIA Kit for Antigen Detection. |
| UPSB949Hu01 | 轉(zhuǎn)化生長因子β3(TGFb3)抗體對 | ELISA; CLIA; ELISPOT; Luminex; Immunochromatography and other Immunoassays. |
| UKSB949Hu01 | 轉(zhuǎn)化生長因子β3(TGFb3)檢測試劑盒DIY材料(酶聯(lián)免疫吸附試驗法) | Main materials for "Do It (ELISA Kit) Yourself". |
參考文獻(xiàn)
| 雜志 | 參考文獻(xiàn) |
| PLoS ONE | A Combinatorial Relative Mass Value Evaluation of Endogenous Bioactive Proteins in Three-Dimensional Cultured Nucleus Pulposus Cells of Herniated Intervertebral Discs: Identification of Potential Target Proteins for Gene Therapeutic Approaches [Plosone: Source] |
| J Reprod Immunol | The immunomodulating effect of seminal plasma on T cells [PubMed: 25799173] |
| Fertility and Sterility | Influence of vitamin D and transforming growth factor β3 serum concentrations, obesity, and family history on the risk for uterine fibroids [pubmed:27743697] |
| Eur J Pharm Biopharm | New scaffolds encapsulating TGF-β3/BMP-7 combinations driving strong chondrogenic differentiation [pubmed:28087378] |
| Transforme edici büyüme fakt?rü-beta-1 n?tralizan antikoru ve transforme edici büyüme fakt?rü-beta-3'ün trakeal darl?k geli?imi üzerine etkileri [10.5606/tgkdc.dergisi.2017.14302] | |
| Fertility and Sterility | Ulipristal acetate decreases transforming growth factor β3 serum and tumor tissue concentrations in patients with uterine fibroids [Pubmed:29525690] |
| Scientific Reports | TGF-β concentrations and activity are down-regulated in the aqueous humor of patients with neovascular age-related macular degeneration [Pubmed:29795291] |
| Immunology?Letters | The involvement of multifunctional TGF-β and related cytokines in pathogenesis of endometriosis [Pubmed: 30367890] |
| Cell?Reports | Transforming Growth Factor-β3 Regulates Adipocyte Number in Subcutaneous White Adipose Tissue [Pubmed: 30332637] |
| INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES | Temporal TGF-β Supergene Family Signalling Cues Modulating Tissue Morphogenesis: Chondrogenesis within a Muscle Tissue Model? [Pubmed: 32660137] |
| Equine Vet J | Limited added value of negative pressure wound therapy compared to calcium alginate dressings for second intention healing in a non?\contaminated and??- [34115409] |
| AAPS PharmSciTech | Nanoparticles and Nanostructured Films with TGF-β3: Preparation, Characterization, and Efficacy [34378118] |
