C反應(yīng)蛋白(CRP)檢測(cè)試劑盒(酶聯(lián)免疫吸附試驗(yàn)法,高敏型)
High Sensitive ELISA Kit for C Reactive Protein (CRP)
C-RP; PTX1; Pentraxin-Related; Pentraxin 1
- 編號(hào)UHEA821Ga
- 物種Chicken (Gallus，雞) 相同的名稱，不同的物種。
- 實(shí)驗(yàn)方法雙抗夾心
- 反應(yīng)時(shí)長(zhǎng)3h
- 檢測(cè)范圍62.5-4,000pg/mL
- 靈敏度最小可檢測(cè)劑量小于等于24.4pg/mL.
- 樣本類型serum, plasma and other biological fluids
- 下載 英文說明書 中文說明書
- 規(guī)格 48T96T 96T*5 96T*10 96T*100
- 價(jià)格 ¥ 1579 ¥ 2256 ¥ 10152 ¥ 19176 ¥ 157920
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產(chǎn)品包裝(模擬)
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產(chǎn)品包裝(模擬)
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實(shí)驗(yàn)結(jié)果圖
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標(biāo)準(zhǔn)曲線圖
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通過ISO 9001、ISO 13485質(zhì)量體系認(rèn)證
特異性
本試劑盒用于檢測(cè)C反應(yīng)蛋白(CRP)檢測(cè)試劑盒(酶聯(lián)免疫吸附試驗(yàn)法,高敏型)，經(jīng)檢測(cè)與其它相似物質(zhì)無明顯交叉反應(yīng)。
由于受到技術(shù)及樣本來源的限制，不可能完成對(duì)所有相關(guān)或相似物質(zhì)交叉反應(yīng)檢測(cè)，因此本試劑盒有可能與未經(jīng)檢測(cè)的其它物質(zhì)有交叉反應(yīng)。
回收率
分別于定值血清及血漿樣本中加入一定量的C反應(yīng)蛋白(CRP)檢測(cè)試劑盒(酶聯(lián)免疫吸附試驗(yàn)法,高敏型)（加標(biāo)樣品），重復(fù)測(cè)定并計(jì)算其均值，回收率為測(cè)定值與理論值的比率。
| 樣本 | 回收率范圍(%) | 平均回收率(%) |
| serum(n=5) | 83-91 | 86 |
| EDTA plasma(n=5) | 83-93 | 87 |
| heparin plasma(n=5) | 79-104 | 101 |
精密度
精密度用樣品測(cè)定值的變異系數(shù)CV表示。CV(%) = SD/mean×100
批內(nèi)差：取同批次試劑盒對(duì)低、中、高值定值樣本進(jìn)行定量檢測(cè),每份樣本連續(xù)測(cè)定20 次，分別計(jì)算不同濃度樣本的平均值及SD值。
批間差：選取3個(gè)不同批次的試劑盒分別對(duì)低、中、高值定值樣本進(jìn)行定量測(cè)定，每個(gè)樣本使用同一試劑盒重復(fù)測(cè)定8次，分別計(jì)算不同濃度樣本的平均值及SD值。
批內(nèi)差： CV<10%
批間差： CV<12%
線性
在定值血清及血漿樣本內(nèi)加入適量的C反應(yīng)蛋白(CRP)檢測(cè)試劑盒(酶聯(lián)免疫吸附試驗(yàn)法,高敏型)，并倍比稀釋成1:2，1:4，1:8，1:16的待測(cè)樣本，線性范圍即為稀釋后樣本中C反應(yīng)蛋白(CRP)檢測(cè)試劑盒(酶聯(lián)免疫吸附試驗(yàn)法,高敏型)含量的測(cè)定值與理論值的比率。
| 樣本 | 1:2 | 1:4 | 1:8 | 1:16 |
| serum(n=5) | 95-103% | 79-98% | 85-96% | 92-101% |
| EDTA plasma(n=5) | 98-105% | 82-90% | 85-96% | 78-89% |
| heparin plasma(n=5) | 80-90% | 81-99% | 96-103% | 84-102% |
穩(wěn)定性
經(jīng)測(cè)定，試劑盒在有效期內(nèi)按推薦溫度保存，其活性降低率小于5%。
為減小外部因素對(duì)試劑盒破壞前后檢測(cè)值的影響，實(shí)驗(yàn)室的環(huán)境條件需盡量保持一致，尤其是實(shí)驗(yàn)室內(nèi)溫度、濕度及溫育條件。其次由同一實(shí)驗(yàn)員來進(jìn)行操作可減少人為誤差。
實(shí)驗(yàn)流程
1. 實(shí)驗(yàn)前標(biāo)準(zhǔn)品、試劑及樣本的準(zhǔn)備；
2. 加樣（標(biāo)準(zhǔn)品及樣本）100µL，37°C孵育1小時(shí)；
3. 吸棄，加檢測(cè)溶液A100µL，37°C孵育1小時(shí)；
4. 洗板3次；
5. 加檢測(cè)溶液B100µL，37°C孵育30分鐘；
6. 洗板5次；
7. 加TMB底物90µL，37°C孵育10-20分鐘；
8. 加終止液50µL，立即450nm讀數(shù)。
實(shí)驗(yàn)原理
將C反應(yīng)蛋白(CRP)檢測(cè)試劑盒(酶聯(lián)免疫吸附試驗(yàn)法,高敏型)抗體包被于96孔微孔板中，制成固相載體，向微孔中分別加入標(biāo)準(zhǔn)品或標(biāo)本，其中的C反應(yīng)蛋白(CRP)檢測(cè)試劑盒(酶聯(lián)免疫吸附試驗(yàn)法,高敏型)與連接于固相載體上的抗體結(jié)合，然后加入生物素化的C反應(yīng)蛋白(CRP)檢測(cè)試劑盒(酶聯(lián)免疫吸附試驗(yàn)法,高敏型)抗體，將未結(jié)合的生物素化抗體洗凈后，加入HRP標(biāo)記的親和素，再次徹底洗滌后加入TMB底物顯色。TMB在過氧化物酶的催化下轉(zhuǎn)化成藍(lán)色，并在酸的作用下轉(zhuǎn)化成最終的黃色。顏色的深淺和樣品中的C反應(yīng)蛋白(CRP)檢測(cè)試劑盒(酶聯(lián)免疫吸附試驗(yàn)法,高敏型)呈正相關(guān)。用酶標(biāo)儀在450nm波長(zhǎng)下測(cè)定吸光度(O.D.值)，計(jì)算樣品濃度。
贈(zèng)品
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相關(guān)產(chǎn)品
| 編號(hào) | 適用物種：Chicken (Gallus，雞) | 應(yīng)用(僅供研究使用，不用于臨床診斷！) |
| UAPA821Ga01 | C反應(yīng)蛋白(CRP)活性蛋白 | Cell?culture;?Activity?Assays. |
| URPA821Ga01 | C反應(yīng)蛋白(CRP)重組蛋白 | Positive Control; Immunogen; SDS-PAGE; WB. |
| UPAA821Ga01 | C反應(yīng)蛋白(CRP)多克隆抗體 | WB; ICC/IF |
| UMAA821Ga21 | C反應(yīng)蛋白(CRP)單克隆抗體 | WB; IHC; ICC; IP. |
| USEA821Ga | C反應(yīng)蛋白(CRP)檢測(cè)試劑盒(酶聯(lián)免疫吸附試驗(yàn)法) | Enzyme-linked immunosorbent assay for Antigen Detection. |
| UHEA821Ga | C反應(yīng)蛋白(CRP)檢測(cè)試劑盒(酶聯(lián)免疫吸附試驗(yàn)法,高敏型) | Enzyme-linked immunosorbent assay for Antigen Detection. |
| ULMA821Ga | C反應(yīng)蛋白(CRP)等多因子檢測(cè)試劑盒(流式熒光發(fā)光法) | FLIA Kit for Antigen Detection. |
| UPGA821Ga01 | C反應(yīng)蛋白(CRP)引物對(duì) | PCR |
| UCGA821Ga01 | C反應(yīng)蛋白(CRP)cDNA | PCR、蛋白表達(dá)等 |
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| МАРКЕРЫ КОСТНОГО РЕМОДЕЛИРОВАНИЯ ПРИ ЗАМЕЩЕНИИ ДЕФЕКТАТРАБЕКУЛЯРНОЙ КОСТНОЙ ТКАНИ РЕЗОРБИРУЕМЫМИ И?… [] | |
| J Transl Med | Multi-omic signatures of atherogenic dyslipidaemia: pre-clinical target identification and validation in humans [33407555] |
| INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES | Use of Fourier-Transform Infrared Spectroscopy (FT-IR) for Monitoring Experimental Helicobacter pylori Infection and Related Inflammatory Response in Guinea Pig??- [33396581] |
| Sci Rep | Ileal transposition helps to regulate plasma hepatokine levels in obese Zucker (Crl: ZUC (ORL)-Lepr fa) rats [33833309] |
| Mol Med Rep | Baicalin relieves Mycoplasma pneumoniae infection?induced lung injury through regulating microRNA?221 to inhibit the TLR4/NF?|êB signaling pathway [34109422] |
| Nutrients | Prevalence and Factors Associated with Iron Deficiency and Anemia among Residents of Urban Areas of S?o Paulo, Brazil [34072813] |
| peerj | Perfluorooctane sulfonate exerts inflammatory bowel disease-like intestinal injury in rats [33510972] |
| Biomed Pharmacother | Intravenous ferric carboxymaltose does not provide benefits in reperfused acute myocardial infarction in the rat with normal iron status [34243097] |
| Aging | Pim-2 kinase inhibits inflammation by suppressing the mTORC1 pathway in atherosclerosis [34547720] |
| Analytical and Bioanalytical Chemistry | Nets of biotin-derived gold nanoparticles as a label for the C-reactive protein immunoassay [34559243] |
| J Oleo Sci | Cardio-protective and Anti-atherosclerosis Effect of Crocetin on Vitamin D3 and HFD-induced Atherosclerosis in Rats [34615830] |
| Medicine | Early-stage serum Stanniocalcin 1 as a predictor of outcome in patients with aneurysmal subarachnoid hemorrhage [Pubmed:34941085] |
| Talanta | A rapid fluorescent aptasensor for point-of-care detection of C-reactive protein [Pubmed:35714415] |
| Experimental and Therapeutic Medicine | Construction of a rabbit model with vinorelbine administration via peripherally inserted central catheter and dynamic monitoring of changes in phlebitis and thrombosis [Pubmed:35126715] |
| Sensors and Actuators B: Chemical | A Two-step Competition Assay for Visual, Sensitive and Quantitative C-reactive Protein Detection in Low-cost Microfluidic Particle Accumulators [] |
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