睪酮(Testo)檢測試劑盒(酶聯(lián)免疫吸附試驗法,快捷型)
Instant ELISA Kit for Testosterone (Testo)
T; Testesteron; Testeronun
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產(chǎn)品包裝(模擬)
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產(chǎn)品包裝(模擬)
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實驗結(jié)果圖
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通過ISO 9001、ISO 13485質(zhì)量體系認(rèn)證
特異性
本試劑盒用于檢測睪酮(Testo)檢測試劑盒(酶聯(lián)免疫吸附試驗法,快捷型)，經(jīng)檢測與其它相似物質(zhì)無明顯交叉反應(yīng)。
由于受到技術(shù)及樣本來源的限制，不可能完成對所有相關(guān)或相似物質(zhì)交叉反應(yīng)檢測，因此本試劑盒有可能與未經(jīng)檢測的其它物質(zhì)有交叉反應(yīng)。
回收率
分別于定值血清及血漿樣本中加入一定量的睪酮(Testo)檢測試劑盒(酶聯(lián)免疫吸附試驗法,快捷型)（加標(biāo)樣品），重復(fù)測定并計算其均值，回收率為測定值與理論值的比率。
| 樣本 | 回收率范圍(%) | 平均回收率(%) |
| serum(n=5) | 94-101 | 98 |
| EDTA plasma(n=5) | 84-93 | 89 |
| heparin plasma(n=5) | 87-94 | 91 |
精密度
精密度用樣品測定值的變異系數(shù)CV表示。CV(%) = SD/mean×100
批內(nèi)差：取同批次試劑盒對低、中、高值定值樣本進(jìn)行定量檢測,每份樣本連續(xù)測定20 次，分別計算不同濃度樣本的平均值及SD值。
批間差：選取3個不同批次的試劑盒分別對低、中、高值定值樣本進(jìn)行定量測定，每個樣本使用同一試劑盒重復(fù)測定8次，分別計算不同濃度樣本的平均值及SD值。
批內(nèi)差： CV<10%
批間差： CV<12%
線性
在定值血清及血漿樣本內(nèi)加入適量的睪酮(Testo)檢測試劑盒(酶聯(lián)免疫吸附試驗法,快捷型)，并倍比稀釋成1:2，1:4，1:8，1:16的待測樣本，線性范圍即為稀釋后樣本中睪酮(Testo)檢測試劑盒(酶聯(lián)免疫吸附試驗法,快捷型)含量的測定值與理論值的比率。
| 樣本 | 1:2 | 1:4 | 1:8 | 1:16 |
| serum(n=5) | 98-105% | 97-105% | 78-101% | 78-89% |
| EDTA plasma(n=5) | 88-95% | 93-102% | 97-105% | 88-98% |
| heparin plasma(n=5) | 82-94% | 78-102% | 95-104% | 81-90% |
穩(wěn)定性
經(jīng)測定，試劑盒在有效期內(nèi)按推薦溫度保存，其活性降低率小于5%。
為減小外部因素對試劑盒破壞前后檢測值的影響，實驗室的環(huán)境條件需盡量保持一致，尤其是實驗室內(nèi)溫度、濕度及溫育條件。其次由同一實驗員來進(jìn)行操作可減少人為誤差。
實驗流程
1. 實驗前標(biāo)準(zhǔn)品、試劑及樣本的準(zhǔn)備；
2. 加樣（標(biāo)準(zhǔn)品及樣本）100µL，37°C溫育30分鐘;
3. 甩干，加檢測溶液A100µL，37°C溫育30分鐘;
4. 洗板3次；
5. 加檢測溶液B100µL， 37°C孵育10分鐘；
6. 洗板5次；
7. 加TMB底物90µL，37°C孵育10－20分鐘；
8. 加終止液50µL，立即450nm讀數(shù)。
實驗原理
將睪酮(Testo)檢測試劑盒(酶聯(lián)免疫吸附試驗法,快捷型)單克隆抗體包被于96孔微孔板中，制成固相載體，向微孔中加入標(biāo)準(zhǔn)品或標(biāo)本以及同時加入HRP標(biāo)記睪酮(Testo)檢測試劑盒(酶聯(lián)免疫吸附試驗法,快捷型)的多克隆抗體，孵育結(jié)束徹底洗滌后加入TMB底物顯色。TMB在過氧化物酶的催化下轉(zhuǎn)化成藍(lán)色，并在酸的作用下轉(zhuǎn)化成最終的黃色。顏色的深淺和樣品中的被檢測物濃度呈正相關(guān)。用酶標(biāo)儀在450nm波長下測定吸光度(O.D.值)，計算樣品濃度。
增值服務(wù)
相關(guān)產(chǎn)品
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| UCPA458Ge21 | 睪酮(Testo)卵白蛋白偶聯(lián)物 | Immunogen; SDS-PAGE; WB. |
| UPAA458Ge01 | 睪酮(Testo)多克隆抗體 | ELISA |
| UPAA458Ge02 | 睪酮(Testo)多克隆抗體 | ELISA, CLIA. / IHC-Fr, ICC, IP (predicted). |
| ULAA458Ge71 | 睪酮(Testo)多克隆抗體(生物素標(biāo)記) | WB; IHC; ICC. |
| UMAA458Ge27 | 睪酮(Testo)單克隆抗體 | ELISA, CLIA. / IHC-Fr, ICC, IP (predicted). |
| UMAA458Ge22 | 睪酮(Testo)單克隆抗體 | ELISA, CLIA. / IHC-Fr, ICC, IP (predicted). |
| UMAA458Ge23 | 睪酮(Testo)單克隆抗體 | ELISA, CLIA. / IHC-Fr, ICC, IP (predicted). |
| UMAA458Ge24 | 睪酮(Testo)單克隆抗體 | ELISA, CLIA. / IHC-Fr, ICC, IP (predicted). |
| UMAA458Ge25 | 睪酮(Testo)單克隆抗體 | ELISA, CLIA. / IHC-Fr, ICC, IP (predicted). |
| UMAA458Ge21 | 睪酮(Testo)單克隆抗體 | ELISA |
| UMEA458Ge | 睪酮(Testo)檢測試劑盒(酶聯(lián)免疫吸附試驗法,小樣本) | Enzyme-linked immunosorbent assay for Antigen Detection. |
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| UKSA458Ge11 | 睪酮(Testo)檢測試劑盒DIY材料(酶聯(lián)免疫吸附試驗法) | Main materials for "Do It (ELISA Kit) Yourself". |
| UKSA458Ge12 | 睪酮(Testo)檢測試劑盒DIY材料(酶聯(lián)免疫吸附試驗法) | Main materials for "Do It (ELISA Kit) Yourself". |
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