激活素A(ACVA)等多因子檢測試劑盒(流式熒光發光法)
Multiplex Assay Kit for Activin A (ACVA) ,etc. by FLIA (Flow Luminescence Immunoassay)
ACV-A; Activin Beta A Beta A Homodimer
（注：單次混測多因子不超過8個指標 ）
- 編號ULMA001Ra
- 物種Rattus norvegicus (Rat，大鼠) 相同的名稱，不同的物種。
- 實驗方法競爭抑制
- 反應時長1.5h
- 檢測范圍0.98-1000pg/mL
- 靈敏度最小可檢測劑量小于等于0.327 pg/mL.
- 樣本類型Serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
- 下載 英文說明書 中文說明書
- 規格 8指標數 7指標數 6指標數 5指標數 4指標數 3指標數 2指標數1指標數
- 價格 ¥ 1281 ¥ 1330 ¥ 1404 ¥ 1502 ¥ 1601 ¥ 1749 ¥ 1970 ¥ 2463 添加到價格計算器 計算器
- 欲了解實際交易價格和更多情況，請與當地經銷商聯系!
-
產品包裝(模擬)
-
產品包裝(模擬)
-
通過ISO 9001、ISO 13485質量體系認證
特異性
本試劑盒用于檢測激活素A(ACVA)等多因子檢測試劑盒(流式熒光發光法)，經檢測與其它相似物質無明顯交叉反應。
由于受到技術及樣本來源的限制，不可能完成對所有相關或相似物質交叉反應檢測，因此本試劑盒有可能與未經檢測的其它物質有交叉反應。
回收率
分別于定值血清及血漿樣本中加入一定量的激活素A(ACVA)等多因子檢測試劑盒(流式熒光發光法)（加標樣品），重復測定并計算其均值，回收率為測定值與理論值的比率。
| 樣本 | 回收率范圍(%) | 平均回收率(%) |
| serum(n=5) | 86-99 | 89 |
| EDTA plasma(n=5) | 84-97 | 88 |
| heparin plasma(n=5) | 81-96 | 88 |
精密度
精密度用樣品測定值的變異系數CV表示。CV(%) = SD/mean×100
批內差：取同批次試劑盒對低、中、高值定值樣本進行定量檢測,每份樣本連續測定20 次，分別計算不同濃度樣本的平均值及SD值。
批間差：選取3個不同批次的試劑盒分別對低、中、高值定值樣本進行定量測定，每個樣本使用同一試劑盒重復測定8次，分別計算不同濃度樣本的平均值及SD值。
批內差： CV<10%
批間差： CV<12%
線性
在定值血清及血漿樣本內加入適量的激活素A(ACVA)等多因子檢測試劑盒(流式熒光發光法)，并倍比稀釋成1:2，1:4，1:8，1:16的待測樣本，線性范圍即為稀釋后樣本中激活素A(ACVA)等多因子檢測試劑盒(流式熒光發光法)含量的測定值與理論值的比率。
| 樣本 | 1:2 | 1:4 | 1:8 | 1:16 |
| serum(n=5) | 94-103% | 85-103% | 85-101% | 96-105% |
| EDTA plasma(n=5) | 88-102% | 79-96% | 83-95% | 81-104% |
| heparin plasma(n=5) | 83-99% | 85-93% | 81-98% | 94-101% |
穩定性
經測定，試劑盒在有效期內按推薦溫度保存，其活性降低率小于5%。
為減小外部因素對試劑盒破壞前后檢測值的影響，實驗室的環境條件需盡量保持一致，尤其是實驗室內溫度、濕度及溫育條件。其次由同一實驗員來進行操作可減少人為誤差。
實驗流程
1. 實驗前標準品、試劑及樣本準備；
2. 加樣（標準品、樣本、磁珠、檢測溶液A）標準品或樣本50μL及磁珠10μL，加檢測溶液A50μL，
37°C酶標板振蕩器孵育60分鐘；
3. 磁吸洗板3次；
4. 加檢測溶液B100μL，37°C振動孵育30分鐘；
5. 磁吸洗板3次；
6. 加鞘液100μL，旋渦震蕩2分鐘后讀數。
實驗原理
將激活素A(ACVA)等多因子檢測試劑盒(流式熒光發光法)抗體包被于磁珠，制成固相載體，向微孔中分別加入標準品或標本以及磁珠、標記VEGFA，標記的激活素A(ACVA)等多因子檢測試劑盒(流式熒光發光法)和未標記的激活素A(ACVA)等多因子檢測試劑盒(流式熒光發光法)與連接于固相載體上的抗體競爭結合，然后將未結合物洗凈后，加入PE標記的親和素，再次徹底洗滌后即可上機讀數。MFI值和樣品中的激活素A(ACVA)等多因子檢測試劑盒(流式熒光發光法)呈負相關。
贈品
增值服務
相關產品
| 編號 | 適用物種：Rattus norvegicus (Rat，大鼠) | 應用(僅供研究使用，不用于臨床診斷！) |
| UCEA001Ra | 激活素A(ACVA)檢測試劑盒(酶聯免疫吸附試驗法) | Enzyme-linked immunosorbent assay for Antigen Detection. |
| ULMA001Ra | 激活素A(ACVA)等多因子檢測試劑盒(流式熒光發光法) | FLIA Kit for Antigen Detection. |
參考文獻
| 雜志 | 參考文獻 |
| 13 | Secretory Products From Epicardial Adipose Tissue of Patients With Type 2 Diabetes Mellitus Induce Cardiomyocyte Dysfunction [Ahajournals: Source] |
| J. Pharmacogn | [Article: Hksobf_2015_V46N2_167] |
| United States Patent Application | Methods for Diagnosis and Treatment of Concussion or Brain Injury [2016:0103140] |
| BMC Cancer. | Activins and their related proteins in colon carcinogenesis: insights from early and advanced azoxymethane rat models of colon cancer. [pubmed:27835986] |
| Basic Clin Pharmacol Toxicol. | Magnolol Attenuates Concanavalin A-induced Hepatic Fibrosis, Inhibits CD4+ T Helper 17 (Th17) Cell Differentiation and Suppresses Hepatic Stellate Cell Activation: Blockade of Smad3/Smad4 Signalling. [pubmed:28032440] |
| Basic & Clinical Pharmacology & Toxicology | Magnolol Attenuates Concanavalin A-induced Hepatic Fibrosis, Inhibits CD4 + T Helper 17(Th17) Cell Differentiation and Suppresses Hepatic Stellate Cell Activation: Blockade of Smad3/Smad4 Signalling [doi:10.1111] |
| Journal of Immunology | MicroRNA-27b Modulates Inflammatory Response and Apoptosis during Mycobacterium tuberculosis Infection [Pubmed:29661829] |
| Journal of?Vascular?Surgery | Expression of platelet-derived growth factor B is upregulated in patients with thoracic aortic dissection [Pubmed:29685513] |
| Acta Biochimica et Biophysica Sinica | Toxic effects of arsenic trioxide on protoscoleces through ROS production, and Ca-ER stress-dependent apoptosis [10.1093:abbs] |
| Poultry?Science | The effect of raw and fermented rapeseed cake on the metabolic parameters, immune status, and intestinal morphology of turkeys [Pubmed:29917099] |
| Food Bioscience | Characterization of Phospholipids from Pacific Saury () Viscera and Their Neuroprotective Activity [10.1016:j.fbio.2018.06.002] |
| Current Analytical Chemistry | Single Wall Carbon Nanotube and Magnetic Bead Based Electrochemical Immunosensor for Sensitive Detection of Salivary Secretory Immunoglobulin A [10.2174:2211556006666170525161225] |
| Journal?of?Veterinary?Internal?Medicine | FRIDAY, NOVEMBER 3, 2017 11.30-12.0 BALLROOM AB [:] |
| Universal Journal of Pharmaceutical Research | SEROPREVALENCE OF ANTI-MANNOSE BINDING LECTIN AUTOANTIBODIES IN PATIENTS WITH RHEUMATOID ARTHRITIS IN SANA'A CITY-YEMEN [10.22270:ujpr.v3i2.138] |
| Involvement of RBP4 in diabetic atherosclerosis and the role of vitamin D intervention [:] | |
| Clin Oral Investig.? | Gingival crevicular fluid levels of human beta-defensin-1 in type 2 diabetes mellitus and periodontitis [Pubmed:29713888] |
| Myocardial fibrosis in hypertrophic cardiomyopathy [:] | |
| TELLING TRANSVERSAL COMPETENCES… TO BE PROFESSIONALLY PROMOTED [:] | |
| Protein biomarker panels for detecting colorectal cancer and advanced adenoma [:] | |
| Endothelotropic activity of 4-hydroxy-3, 5-di-tret-butylcinnamic acid in the conditions of experimental cerebral ischemia [:] | |
| Silencing of the CSNK2β gene by siRNA inhibits invasiveness and growth of MDA-MB-231 cells [10.1101:311092] | |
| Duchenne Muscular Dystrophy | Flow Cytometry-Defined CD49d Expression in Circulating T-Lymphocytes Is a Biomarker for Disease Progression in Duchenne Muscular Dystrophy [Pubmed:29067667] |
| S | S [S] |
| P | P [P] |
| D | D [D] |
| h | h [h] |
| O | O [O] |
| M | M [M] |
| E | E [E] |
| I | I [I] |
| B | B [B] |
| T | T [T] |
| N | N [N] |
| [] | |
| [] | |
| L | L [L] |
| J | J [J] |
| Journal of Geriatric?Oncology | Serum biomarkers for predicting overall survival and early mortality in older patients with metastatic solid tumors [Pubmed: 30952517] |
| clinica chimica acta | The performances of serum activins and follistatin in the diagnosis of ectopic pregnancy: A prospective case-control study [Pubmed: 31647905] |
| hindawi | The Association between the Activin A Serum Level and Carotid Intima-Media Thickness in Chronic Kidney Disease Patients [] |
| Pathology & Oncology Research | Profiling Activins and Follistatin in Colorectal Cancer According to Clinical Stage, Tumour Sidedness and Smad4 Status [34867090] |
