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肌動(dòng)蛋白β(ACTB)單克隆抗體

Monoclonal Antibody to Beta Actin (ACTB)

Actin Beta; ACT-B; PS1TP5BP1; Actin, cytoplasmic 1; Actin, cytoplasmic 1, N-terminally processed

編號(hào)UMAB340Hu21
物種Homo sapiens (Human，人) 相同的名稱，不同的物種。
- 所有
- 人
- 小鼠
- 大鼠
- 豚鼠
- 家兔
- 獼猴
- 山羊
- 綿羊
- 馬
- 牛
- 豬
- 雞
- 狗
- 其他物種
- 多物種
- 泛物種
來(lái)源單抗制備
宿主小鼠
效價(jià)(克隆號(hào))C2
Ig亞型 IgG2b Kappa
純化方式蛋白A + 蛋白G親和純化
標(biāo)記物無(wú)標(biāo)記物
免疫原 RPB340Hu01-肌動(dòng)蛋白β(ACTB)重組蛋白
緩沖液成份0.01M 磷酸鹽緩沖液（pH7.4，containing 0.05% Proclin-300，50% glycerol）
性狀液體
濃度1mg/mL
且適物種Sus scrofa; Porcine (Pig，豬)
應(yīng)用WB; IHC; ICC/IF
如果抗體需用于流式細(xì)胞術(shù)，請(qǐng)參見(jiàn)流式抗體。
下載英文說(shuō)明書(shū) 中文說(shuō)明書(shū)
規(guī)格 20μl100μl 200μl 1ml 10ml
價(jià)格 ¥ 376 878 ¥ 878 ¥ 1254 ¥ 3135 ¥ 12540
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產(chǎn)品包裝(模擬)
產(chǎn)品包裝(模擬)

DAB staining on IHC-P; Sample: Human Kidney Tissue; Primary Ab: 10μg/ml Mouse Anti-Human ACTb Antibody Second Ab: 2μg/mL?HRP-Linked Caprine Anti-Mouse IgG Polyclonal Antibody (Catalog: SAA544Mu19)
DAB staining on IHC-P;
Sample: Human Tonsil Tissue;
Primary Ab: 10μg/ml Mouse Anti-Human ACTb Antibody
Second Ab: 2μg/mL?HRP-Linked Caprine Anti-Mouse IgG Polyclonal Antibody
(Catalog: SAA544Mu19)
DAB staining on IHC-P;
Sample: Human Tonsil Tissue
Primary Ab: 10μg/ml Mouse Anti-Human ACTB Antibody
Control: Used PBS instead of primary antibody
Second Ab: 2μg/ml HRP-Linked Caprine Anti-Mouse IgG Polyclonal Antibody
(Catalog: SAA544Mu19)
DAB staining on IHC-P;
Sample: Human Kidney Tissue
Primary Ab: 10μg/ml Mouse Anti-Human ACTB Antibody
Control: Used PBS instead of primary antibody
Second Ab: 2μg/ml HRP-Linked Caprine Anti-Mouse IgG Polyclonal Antibody
(Catalog: SAA544Mu19)
DAB staining on IHC-P;
Sample: Porcine Kidney Tissue
Primary Ab: 20μg/ml Mouse Anti-Human ACTB Antibody
Control: Used PBS instead of primary antibody
Second Ab: 2μg/ml HRP-Linked Caprine Anti-Mouse IgG Polyclonal Antibody
(Catalog: SAA544Mu19)
FITC staining on IF;
Sample: Hela cell
Primary Ab: 30μg/ml Mouse Anti-Human ACTB Antibody
Second Ab: 2μg/ml FITC-Linked Caprine Anti-Mouse IgG Polyclonal Antibody
(Catalog: SAA544Mu11)

Western Blot; Sample: Human Lung lysate; Primary Ab: 3μg/ml Mouse Anti-Human ACTb Antibody Second Ab: 0.2μg/mL?HRP-Linked Caprine Anti-Mouse IgG Polyclonal Antibody (Catalog: SAA544Mu19)
Western Blot; Sample: Hela cell lysate
Primary Ab: 0.1μg/ml Mouse Anti-Human IGF1 Antibody
Second Ab: 0.2μg/mL HRP-Linked Caprine Anti-Mouse IgG Polyclonal Antibody
(Catalog: SAA544Mu19)

通過(guò)ISO 9001、ISO 13485質(zhì)量體系認(rèn)證

特異性

該抗體是針對(duì)ACTB的小鼠單克隆抗體。在免疫組織化學(xué)染色和免疫印跡實(shí)驗(yàn)中能識(shí)別ACTB。

用法

Western blotting: 0.01-3μg/mL;
Immunohistochemistry: 5-20μg/mL;
Immunofluorescence: 5-30μg/mL;
Optimal working dilutions must be determined by end user.

儲(chǔ)存

經(jīng)常使用則4°C保存。-20°C保存不超過(guò)兩年。避免反復(fù)凍融。

穩(wěn)定性

熱穩(wěn)定性以損失率顯示。損失率是由加速降解試驗(yàn)決定，具體方法如下：在37°C孵育48小時(shí)，沒(méi)有顯著的降解或者沉淀產(chǎn)生。保質(zhì)期內(nèi)，在適當(dāng)?shù)臈l件下存儲(chǔ)，損失率低于5%。

贈(zèng)品

增值服務(wù)

抗體標(biāo)記定制服務(wù)
Protein-A/G純化柱
 細(xì)胞和組織染色液
 抗體陽(yáng)性對(duì)照品
 組織/切片定制服務(wù)
磷酸化抗體定制服務(wù)
免疫印跡(WB)實(shí)驗(yàn)服務(wù)
免疫組織化學(xué)(IHC)實(shí)驗(yàn)服務(wù)
免疫細(xì)胞化學(xué)(ICC)實(shí)驗(yàn)服務(wù)
流式細(xì)胞術(shù)(FCM)實(shí)驗(yàn)服務(wù)
免疫沉淀(IP)實(shí)驗(yàn)服務(wù)
免疫熒光(IF)實(shí)驗(yàn)服務(wù)
緩沖液
 DAB顯色試劑盒
 SABC試劑盒
 長(zhǎng)臂生物素標(biāo)記試劑盒
 實(shí)時(shí)熒光定量PCR實(shí)驗(yàn)服務(wù)

參考文獻(xiàn)

雜志	參考文獻(xiàn)
International Journal of Molecular Sciences	Nano-Nutrition of Chicken Embryos. The Effect of in Ovo Administration of Diamond Nanoparticles and L-Glutamine on Molecular Responses in Chicken Embryo Pectoral Muscles [NCBI:PMC3856104]
European Journal of BioMedical Research	Expression of chloride channels in mouse skin incisional wounds [96]
Cell?Metabolism	Targeted Elimination of Senescent Beta Cells Prevents Type 1 Diabetes []
Biological & Pharmaceutical Bulletin	Neuroinflammation, Oxidative Stress, and Neurogenesis in a Mouse Model of Chronic Fatigue Syndrome, and the Treatment with Kampo Medicine [Pubmed: 31902915]
Biological research	Linc-OIP5 in the breast cancer cells regulates angiogenesis of human umbilical vein endothelial cells through YAP1/Notch/NRP1 signaling circuit at a tumor … [Pubmed: 32046779]
JOURNAL OF INVESTIGATIVE MEDICINE	Effect of ischemic postconditioning on cell apoptosis and expression of relevant genes in non-culprit coronary arteries [Pubmed: 32784207]
bioscience biotechnology research asia	Malted Barley Improved the Structure and Function of Gastrocnemius Muscle of Hypercholesterolemic Mother Rats and their Offspring []
Genes	Tryptophan Depletion Modulates Tryptophanyl-tRNA Synthetase-Mediated High-Affinity Tryptophan Uptake into Human Cells [33261077]
Nat Commun	POLRMT mutations impair mitochondrial transcription causing neurological disease [33602924]
Life Sci	Indole-linked 1, 2, 3-triazole derivatives efficiently modulate COX-2 protein levels in human THP-1 monocytes by suppressing AGE-ROS-NF-kβ nexus [Pubmed:34990649]
J Ethnopharmacol	Isolation and characterization of anti-inflammatory and anti-proliferative compound, for B-cell Non-Hodgkin lymphoma, from Nyctanthes arbor-tristis Linn. [Pubmed:35398498]
Multimedia Tools and Applications	Investigation of single beam ultrasound sensitivity as a monitoring tool for local hyperthermia treatment in breast cancer []

留言咨詢

編號(hào)	適用物種：Homo sapiens (Human，人)	應(yīng)用(僅供研究使用，不用于臨床診斷！)
URPB340Hu01	肌動(dòng)蛋白β(ACTB)重組蛋白	Positive Control; Immunogen; SDS-PAGE; WB.
UPAB340Hu01	肌動(dòng)蛋白β(ACTB)多克隆抗體	WB; IHC
UCAB340Hu01	抗肌動(dòng)蛋白β(ACTB)多克隆抗體	WB; IHC
UMAB340Hu22	肌動(dòng)蛋白β(ACTB)單克隆抗體	WB; IHC
UMAB340Hu23	肌動(dòng)蛋白β(ACTB)單克隆抗體	WB; IHC; ICC/IF
UMAB340Hu24	肌動(dòng)蛋白β(ACTB)單克隆抗體	WB
UMAB340Hu21	肌動(dòng)蛋白β(ACTB)單克隆抗體	WB; IHC; ICC/IF
UMAB340Hu25	肌動(dòng)蛋白β(ACTB)單克隆抗體	WB
UCAB340Hu22	抗肌動(dòng)蛋白β(ACTB)單克隆抗體	WB; IHC; ICC/IF