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脂聯(lián)素(ADPN)單克隆抗體

Monoclonal Antibody to Adiponectin (ADPN)

GBP28; ApM1; AdipoQ; Acrp30; ACDC; APM1; C1Q And Collagen Domain Containing; Adipocyte Complement-Related Protein Of 30 KDa; Adipose Most Abundant Gene Transcript 1

編號UMAA605Hu21
物種Homo sapiens (Human，人) 相同的名稱，不同的物種。
- 所有
- 人
- 小鼠
- 大鼠
- 豚鼠
- 家兔
- 獼猴
- 山羊
- 綿羊
- 馬
- 牛
- 豬
- 雞
- 狗
- 其他物種
- 多物種
- 泛物種
來源單抗制備
宿主小鼠
效價(jià)(克隆號)H6
Ig亞型 IgG2b Kappa
純化方式蛋白A + 蛋白G親和純化
標(biāo)記物無標(biāo)記物
免疫原 RPA605Hu01-脂聯(lián)素(ADPN)重組蛋白
緩沖液成份0.01M 磷酸鹽緩沖液（pH7.4，containing 0.05% Proclin-300，50% glycerol）
性狀液體
濃度1mg/ml
且適物種Rattus norvegicus (Rat，大鼠)
應(yīng)用WB; IHC
如果抗體需用于流式細(xì)胞術(shù)，請參見流式抗體。
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產(chǎn)品包裝(模擬)
產(chǎn)品包裝(模擬)

DAB staining on IHC-P; Sample: Human Cardiac Muscle Tissue; Primary Ab: 20μg/ml Mouse Anti-Human ADP Antibody Second Ab: 2μg/mL?HRP-Linked Caprine Anti-Mouse IgG Polyclonal Antibody (Catalog: SAA544Mu19)
DAB staining on IHC-P;
Sample: Human Kidney Tissue;
Primary Ab: 20μg/ml Mouse Anti-Human ADP Antibody
Second Ab: 2μg/mL?HRP-Linked Caprine Anti-Mouse IgG Polyclonal Antibody
(Catalog: SAA544Mu19)
DAB staining on IHC-P;
Sample: Human Cardiac Muscle Tissue
Primary Ab: 20μg/ml Mouse Anti-Human ADPN Antibody
Control: Used PBS instead of primary antibody
Second Ab: 2μg/ml HRP-Linked Caprine Anti-Mouse IgG Polyclonal Antibody
(Catalog: SAA544Mu19)
DAB staining on IHC-P;
Sample: Human Kidney Tissue
Primary Ab: 20μg/ml Mouse Anti-Human ADPN Antibody
Control: Used PBS instead of primary antibody
Second Ab: 2μg/ml HRP-Linked Caprine Anti-Mouse IgG Polyclonal Antibody
(Catalog: SAA544Mu19)

Western Blot Sample: Lane1: Human Serum; Lane2: Rat Heart lysate; Lane3: HepG2 cell lysate Primary Ab: 2μg/ml Mouse Anti-Human ADPN Antibody Second Ab: 0.2μg/mL HRP-Linked Caprine Anti-Mouse IgG Polyclonal Antibody (Catalog: SAA544Mu19)
Western Blot; Samples: Lane1: Human Serum; Lane2: Human Plasma;
Primary Ab: 0.3μg/ml Mouse Anti-Human ADPN Antibody
Second Ab: 0.2μg/ml HRP-Linked Caprine Anti-Mouse IgG Polyclonal Antibody
(Catalog: SAA544Mu19)

通過ISO 9001、ISO 13485質(zhì)量體系認(rèn)證

特異性

該抗體是針對ADPN的小鼠單克隆抗體。在免疫組織化學(xué)染色和免疫印跡實(shí)驗(yàn)中能識別ADPN。

用法

Western blotting: 0.5-2μg/mL
Immunohistochemistry: 5-20μg/mL
Immunocytochemistry: 5-20μg/mL
Optimal working dilutions must be determined by end user.

儲存

經(jīng)常使用則4°C保存。-20°C保存不超過兩年。避免反復(fù)凍融。

穩(wěn)定性

熱穩(wěn)定性以損失率顯示。損失率是由加速降解試驗(yàn)決定，具體方法如下：在37°C孵育48小時(shí)，沒有顯著的降解或者沉淀產(chǎn)生。保質(zhì)期內(nèi)，在適當(dāng)?shù)臈l件下存儲，損失率低于5%。

贈品

增值服務(wù)

抗體標(biāo)記定制服務(wù)
Protein-A/G純化柱
 細(xì)胞和組織染色液
 抗體陽性對照品
 組織/切片定制服務(wù)
磷酸化抗體定制服務(wù)
免疫印跡(WB)實(shí)驗(yàn)服務(wù)
免疫組織化學(xué)(IHC)實(shí)驗(yàn)服務(wù)
免疫細(xì)胞化學(xué)(ICC)實(shí)驗(yàn)服務(wù)
流式細(xì)胞術(shù)(FCM)實(shí)驗(yàn)服務(wù)
免疫沉淀(IP)實(shí)驗(yàn)服務(wù)
免疫熒光(IF)實(shí)驗(yàn)服務(wù)
緩沖液
 DAB顯色試劑盒
 SABC試劑盒
 長臂生物素標(biāo)記試劑盒
 實(shí)時(shí)熒光定量PCR實(shí)驗(yàn)服務(wù)

參考文獻(xiàn)

雜志	參考文獻(xiàn)
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BioMed Research International	Serum Fetuin-A Levels Related with Microalbuminuria in Diet-Induced Obese Rats [PubMed: PMC3591142]
PLoS One	CD40L Deficiency Attenuates Diet-Induced Adipose Tissue Inflammation by Impairing Immune Cell Accumulation and Production of Pathogenic IgG-Antibodies [PubMed: PMC3297623]
Molecular and Cellular Endocrinology	Soy isoflavones modulate adipokines and myokines to regulate lipid metabolism in adipose tissue, skeletal muscle and liver of male Huanjiang mini-pigs [ScienceDirect: S0303720712004194]
British Journal of Nutrition	n-6: n-3 PUFA ratio is involved in regulating lipid metabolism and inflammation in pigs [Pubmed: 23947577]
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Iranian Journal of Pharmaceutical Research	Antihyperglycemic Effect of Rosa Damascena is Mediated by PPAR.γ Gene Expression in Animal Model of Insulin Resistance [pubmed:29201096]
Biomedical & Pharmacology Journal	Association of ADIPOQ Gene Variant Rs266729 with Circulatory Adiponectin levels in Patients with type 2 Diabetes in North Indian Population: A Case-Control Study [DOI:10.13005/bpj/1123]
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Medicine (Baltimore)	Rapid detection of urinary soluble intercellular adhesion molecule-1 for determination of lupus nephritis activity [Pubmed:29953010]
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Hematology	Relationship between obesity and iron deficiency anemia: is there a role of hepcidin? [Pubmed:29318945]
International Journal of Gerontology	Aging effects on circulating adiponectin levels and the expressions of adiponectin and adiponectin receptor 1 in the brains of male rats [10.1016:j.ijge.2018.01.002]
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Nutrients	Effects of Metformin Combined with Lactoferrin on Lipid Accumulation and Metabolism in Mice Fed with High-Fat Diet [Pubmed: 30400147]
Progress in Nutrition	Effect of fermented food consumption on biochemical parameters and adipokines levels [Doi: 0.23751/pn.v20i4.7374]
Biology?of?Reproduction	Recombinant adiponectin alleviates abortion in mice by regulating Th17/Treg imbalance via p38MAPK-STAT5 pathway [Pubmed: 30496353]
Nutrition in the Prevention and Treatment of Abdominal Obesity	Effects of Diet-Induced Early-Stage Obesity on a Low-Testosterone Gottingen Minipig []
Evidence-Based Complementary and Alternative Medicine	BuShenKangShuai Tablet Alleviates Hepatic Steatosis via Improving Liver Adiponectin Resistance in ApoE-/-Mice []
Life?Sciences	Interrelation of liver vascularity to non-alcoholic fatty liver through a comparative study of the vasodilator effect of carvedilol or nicorandil in rats [Pubmed: 30826497]
Arteriosclerosis, Thrombosis, and?Vascular Biology	Cardiac Lymphatic Dysfunction Causes Drug-Eluting Stent–Induced Coronary Hyperconstricting Responses in Pigs In Vivo [Pubmed: 30816801]
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Nutrients	High-dose Glycerol Monolaurate Up-Regulated Beneficial Indigenous Microbiota without Inducing Metabolic Dysfunction and Systemic Inflammation: New Insights?… []
Proceedings of the Royal Society B: Biological Sciences	Paternal chronic folate supplementation induced the transgenerational inheritance of acquired developmental and metabolic changes in chickens [Pubmed: 31506054]
Cytokine	Increased plasma osteopontin levels are associated with nonalcoholic fatty liver disease in patients with type 2 diabetes mellitus [Pubmed: 31514105]
Food & Function	Collaborative Effects of Chlorogenic Acid and Caffeine on Lipid Metabolism via AMPKα-LXRα/SREBP-1c Pathway in High-Fat Diet-Induced Obese Mice [Pubmed: 31670366]
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Annals of Nutrition and Metabolism	Serum Asprosin Concentrations Are Increased and Associated with Insulin Resistance in Children with Obesity [Pubmed: 31775140]
BMC Endocrine Disorders	The role of the adipocytokines vaspin and visfatin in vascular endothelial function and insulin resistance in obese children [Pubmed: 31771561]
Journal of Hazardous Materials	Triphenyl phosphate causes a sexually dimorphic metabolism dysfunction associated with disordered adiponectin receptors in pubertal mice [Pubmed: 31796355]
Experimental and Clinical Endocrinology & Diabetes	HOMA-Adiponectin Closely Associates with Cardiometabolic Risk Markers in Middle-Aged Indians with Metabolic Syndrome [Pubmed: 32189318]
JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY	Cerasus humilis cherry polyphenol reduces high-fat diet-induced obesity in C57BL/6 mice by mitigating fat deposition, inflammation, and oxidation [Pubmed: 32227855]
Nature Communications	AdipoR1/AdipoR2 dual agonist recovers nonalcoholic steatohepatitis and related fibrosis via endoplasmic reticulum-mitochondria axis [Pubmed: 33199780]
Metabolites	Adipocytokines and Metabolic Syndrome in Patients with Schizophrenia [Pubmed: 33066473]
Ann Nutr Metab	Copeptin and Nesfatin-1 Are Interrelated Biomarkers with Roles in the Pathogenesis of Insulin Resistance in Chinese Children with Obesity [Pubmed: 33027789]
aging	Resemblance and differences in dietary restriction nephroprotective mechanisms in young and old rats [Pubmed: 32970613]
Diabetes Metab Syndr Obes	Serum ZAG and Adiponectin Levels Were Closely Related to Obesity and the Metabolically Abnormal Phenotype in Chinese Population [Pubmed: 32904655]
DIABETOLOGIA	Adiponectin treatment improves insulin resistance in mice by regulating the expression of the mitochondrial-derived peptide MOTS-c and its response to exercise via?… [Pubmed: 32880686]
J Endocrinol Invest	Serum glypican4 and glycosylphosphatidylinositol-specific phospholipase D levels are associated with adipose tissue insulin resistance in obese subjects with … [Pubmed: 32816247]
JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY	Highly Branched RG-I Domain Enrichment are Indispensable for Pectin Mitigating Against High-Fat Diet-Induced Obesity [Pubmed: 32633953]
J Endocrinol Invest	Activation of central galanin receptor 2 mitigated insulin resistance in adipocytes of diabetic rats [Pubmed: 32588381]
ARCHIVES OF PHYSIOLOGY AND BIOCHEMISTRY	The effect of nutrition and exercise training on irisin and semaphorin-3E levels in obese patients [Pubmed: 32569480]
Diabetes	Exercise-Induced Increases in Insulin Sensitivity After Bariatric surgery are Mediated by Muscle Extracellular Matrix Remodeling [Pubmed: 32409493]
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Cell Metab	TMBIM1 is an inhibitor of adipogenesis and its depletion promotes adipocyte hyperplasia and improves obesity-related metabolic disease [34107313]
Biomedical and Environmental Sciences	Decreased Plasma MANF Levels are Associated with Type 2 Diabetes [33766221]
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Indian Journal of Forensic Medicine & Toxicology	Altered Serum Marker of Adipokines Profile in Breast Cancer Women []
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Phytomedicine	Smilax china L. flavonoid alleviates HFHS-induced inflammation by regulating the gut-liver axis in mice [34561124]
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Animals	Effects of Obesity on Adiponectin System Skin Expression in Dogs: A Comparative Study [34438765]
Cell Death & Disease	Hepatic deficiency of selenoprotein S exacerbates hepatic steatosis and insulin resistance [Pubmed:35347118]
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BioMed Research International	Adiponectin Levels Are Associated with White Matter Lesions (WMLs) and Cognitive Impairment [Pubmed:35087911]
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Scientific Reports	SARS-CoV2 Induced Biochemical Mechanisms in Liver Damage and Intestinal Lesions. []

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編號	適用物種：Homo sapiens (Human，人)	應(yīng)用(僅供研究使用，不用于臨床診斷！)
URPA605Hu01	脂聯(lián)素(ADPN)重組蛋白	Positive Control; Immunogen; SDS-PAGE; WB.
UEPA605Hu61	脂聯(lián)素(ADPN)真核蛋白	Positive Control; Immunogen; SDS-PAGE; WB.
URPA605Hu02	脂聯(lián)素(ADPN)重組蛋白	Positive Control; Immunogen; SDS-PAGE; WB.
UCPA605Hu21	脂聯(lián)素(ADPN)卵白蛋白偶聯(lián)物	Immunogen; SDS-PAGE; WB.
UPAA605Hu01	脂聯(lián)素(ADPN)多克隆抗體	WB; IHC; ICC/IF
UPAA605Hu06	脂聯(lián)素(ADPN)多克隆抗體	WB
UPAA605Hu08	脂聯(lián)素(ADPN)多克隆抗體	WB; IHC; ICC; IP.
ULAA605Hu71	脂聯(lián)素(ADPN)多克隆抗體(生物素標(biāo)記)	WB
ULAA605Hu81	脂聯(lián)素(ADPN)多克隆抗體(異硫氰酸熒光素標(biāo)記)	WB; IHC; ICC; IF.
UMAA605Hu21	脂聯(lián)素(ADPN)單克隆抗體	WB; IHC
UMAA605Hu25	脂聯(lián)素(ADPN)單克隆抗體	WB; IHC
UMAA605Hu26	脂聯(lián)素(ADPN)單克隆抗體	ICC/IF
UMAA605Hu22	脂聯(lián)素(ADPN)單克隆抗體	WB; IHC
UMAA605Hu23	脂聯(lián)素(ADPN)單克隆抗體	WB; IHC
UMAA605Hu24	脂聯(lián)素(ADPN)單克隆抗體	WB; IHC
UFAA605Hu02	抗脂聯(lián)素(ADPN)單克隆抗體	FCM
UFAA605Hu04	抗脂聯(lián)素(ADPN)單克隆抗體	FCM
UMEA605Hu	脂聯(lián)素(ADPN)檢測試劑盒(酶聯(lián)免疫吸附試驗(yàn)法,小樣本)	Enzyme-linked immunosorbent assay for Antigen Detection.
USEA605Hu	脂聯(lián)素(ADPN)檢測試劑盒(酶聯(lián)免疫吸附試驗(yàn)法)	Enzyme-linked immunosorbent assay for Antigen Detection.
USCA605Hu	脂聯(lián)素(ADPN)檢測試劑盒(化學(xué)發(fā)光免疫分析法)	Chemiluminescent immunoassay for Antigen Detection.
ULMA605Hu	脂聯(lián)素(ADPN)等多因子檢測試劑盒(流式熒光發(fā)光法)	FLIA Kit for Antigen Detection.
UPSA605Hu01	脂聯(lián)素(ADPN)抗體對	ELISA; CLIA; ELISPOT; Luminex; Immunochromatography and other Immunoassays.
UKSA605Hu01	脂聯(lián)素(ADPN)檢測試劑盒DIY材料(酶聯(lián)免疫吸附試驗(yàn)法)	Main materials for "Do It (ELISA Kit) Yourself".