連環(huán)蛋白β1(β-catenin)單克隆抗體
Monoclonal Antibody to Beta Catenin (β-catenin)
CTNNb1; bCat; b-Cat; CTNN-B1; Catenin Beta 1; Cadherin-Associated Protein
- 編號(hào)UMAB021Hu21
- 物種Homo sapiens (Human，人) 相同的名稱(chēng)，不同的物種。
- 來(lái)源單抗制備
- 宿主小鼠
- 效價(jià)(克隆號(hào))C4
- Ig亞型 IgG2a Kappa
- 純化方式蛋白A + 蛋白G親和純化
- 標(biāo)記物無(wú)標(biāo)記物
- 免疫原 RPB021Hu01-連環(huán)蛋白β1(β-catenin)重組蛋白
- 緩沖液成份磷酸鹽緩沖液（pH7.4）
- 性狀液體
- 濃度1mg/mL
- 且適物種Mus musculus (Mouse，小鼠), Rattus norvegicus (Rat，大鼠), Cavia (Guinea pig ，豚鼠), Canis familiaris; Canine (Dog，犬), Sus scrofa; Porcine (Pig，豬), Bos taurus; Bovine (Cattle，牛), Capra hircus; Caprine (Goat，山羊), Ovis aries; Ovine (Sheep，綿羊)
- 應(yīng)用WB; IHC
如果抗體需用于流式細(xì)胞術(shù)，請(qǐng)參見(jiàn)流式抗體。 - 下載 英文說(shuō)明書(shū) 中文說(shuō)明書(shū)
- 規(guī)格 20μl100μl 200μl 1ml 10ml
- 價(jià)格 ¥ 376 ¥ 878 ¥ 1254 ¥ 3135 ¥ 12540
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產(chǎn)品包裝(模擬)
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產(chǎn)品包裝(模擬)
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DAB staining on IHC-P; Sample: Human Breast cancer Tissue; Primary Ab: 20μg/ml Mouse Anti-Human β-catenin Antibody Second Ab: 2μg/mL?HRP-Linked Caprine Anti-Mouse IgG Polyclonal Antibody (Catalog: SAA544Mu19)
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DAB staining on IHC-P;
Sample: Human Liver cancer Tissue;
Primary Ab: 20μg/ml Mouse Anti-Human β-catenin Antibody
Second Ab: 2μg/mL?HRP-Linked Caprine Anti-Mouse IgG Polyclonal Antibody
(Catalog: SAA544Mu19) -
DAB staining on IHC-P;
Sample: Human Liver Tissue;
Primary Ab: 20μg/ml Mouse Anti-Human β-catenin Antibody
Second Ab: 2μg/mL?HRP-Linked Caprine Anti-Mouse IgG Polyclonal Antibody
(Catalog: SAA544Mu19) -
DAB staining on IHC-P;
Sample: Human Stomach Tissue;
Primary Ab: 20μg/ml Mouse Anti-Human β-catenin Antibody
Second Ab: 2μg/mL?HRP-Linked Caprine Anti-Mouse IgG Polyclonal Antibody
(Catalog: SAA544Mu19)
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Western Blot; Sample: Lane1: Mouse Cerebrum lysate; Lane2: Human Lung lysate; Lane3: A549 cell lysate Primary Ab: 0.2μg/ml Mouse Anti-Human β-catenin Antibody Second Ab: 0.2μg/mL HRP-Linked Caprine Anti-Mouse IgG Polyclonal Antibody (Catalog: SAA544Mu19)
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Western Blot; Sample: Lane1: Porcine Heart lysate; Lane2: Porcine Cerebrum lysate; Lane3: Rat Cerebrum lysate; Lane4: Bovine Heart lysate; Lane5: Bovine Cerebrum lysate; Lane6: Canine Heart lysate; Lane7: Canine Cerebrum lysate; Lane8: Caprine Heart lysate; Lane9: Caprine Cerebrum lysate; Lane10: Cavia Heart lysate; Lane11: Cavia Cerebrum lysate
Primary Ab: 2μg/ml Mouse Anti-Human β-catenin Antibody
Second Ab: 0.2μg/mL HRP-Linked Caprine Anti-Mouse IgG Polyclonal Antibody
(Catalog: SAA544Mu19)
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通過(guò)ISO 9001、ISO 13485質(zhì)量體系認(rèn)證
特異性
該抗體是針對(duì)β-catenin的小鼠單克隆抗體。在免疫組織化學(xué)染色和免疫印跡實(shí)驗(yàn)中能識(shí)別β-catenin。
用法
Western blotting: 0.01-2μg/mL
Immunohistochemistry: 5-20μg/mL
Immunocytochemistry: 5-20μg/mL
Optimal working dilutions must be determined by end user.
儲(chǔ)存
經(jīng)常使用則4°C保存。-20°C保存不超過(guò)兩年。避免反復(fù)凍融。
穩(wěn)定性
熱穩(wěn)定性以損失率顯示。損失率是由加速降解試驗(yàn)決定，具體方法如下：在37°C孵育48小時(shí)，沒(méi)有顯著的降解或者沉淀產(chǎn)生。保質(zhì)期內(nèi)，在適當(dāng)?shù)臈l件下存儲(chǔ)，損失率低于5%。
贈(zèng)品
增值服務(wù)
抗體標(biāo)記定制服務(wù)
Protein-A/G純化柱
細(xì)胞和組織染色液
抗體陽(yáng)性對(duì)照品
組織/切片定制服務(wù)
磷酸化抗體定制服務(wù)
免疫印跡(WB)實(shí)驗(yàn)服務(wù)
免疫組織化學(xué)(IHC)實(shí)驗(yàn)服務(wù)
免疫細(xì)胞化學(xué)(ICC)實(shí)驗(yàn)服務(wù)
流式細(xì)胞術(shù)(FCM)實(shí)驗(yàn)服務(wù)
免疫沉淀(IP)實(shí)驗(yàn)服務(wù)
免疫熒光(IF)實(shí)驗(yàn)服務(wù)
緩沖液
DAB顯色試劑盒
SABC試劑盒
長(zhǎng)臂生物素標(biāo)記試劑盒
實(shí)時(shí)熒光定量PCR實(shí)驗(yàn)服務(wù)
相關(guān)產(chǎn)品
| 編號(hào) | 適用物種：Homo sapiens (Human，人) | 應(yīng)用(僅供研究使用，不用于臨床診斷！) |
| URPB021Hu01 | 連環(huán)蛋白β1(β-catenin)重組蛋白 | Positive Control; Immunogen; SDS-PAGE; WB. |
| URPB021Hu02 | 連環(huán)蛋白β1(β-catenin)重組蛋白 | Positive Control; Immunogen; SDS-PAGE; WB. |
| UPAB021Hu01 | 連環(huán)蛋白β1(β-catenin)多克隆抗體 | WB; IHC |
| UCAB021Hu01 | 抗連環(huán)蛋白β1(β-catenin)多克隆抗體 | WB; IHC |
| UMAB021Hu23 | 連環(huán)蛋白β1(β-catenin)單克隆抗體 | WB; IHC |
| UMAB021Hu24 | 連環(huán)蛋白β1(β-catenin)單克隆抗體 | WB; IHC |
| UMAB021Hu25 | 連環(huán)蛋白β1(β-catenin)單克隆抗體 | WB; IHC |
| UMAB021Hu27 | 連環(huán)蛋白β1(β-catenin)單克隆抗體 | WB; IHC |
| UMAB021Hu21 | 連環(huán)蛋白β1(β-catenin)單克隆抗體 | WB; IHC |
| UMAB021Hu22 | 連環(huán)蛋白β1(β-catenin)單克隆抗體 | WB; IHC |
| UMAB021Hu26 | 連環(huán)蛋白β1(β-catenin)單克隆抗體 | WB |
| UCAB021Hu22 | 抗連環(huán)蛋白β1(β-catenin)單克隆抗體 | WB; IHC |
| UMAB021Hu29 | 連環(huán)蛋白β1(β-catenin)單克隆抗體 | WB |
| USEB021Hu | 連環(huán)蛋白β1(β-catenin)檢測(cè)試劑盒(酶聯(lián)免疫吸附試驗(yàn)法) | Enzyme-linked immunosorbent assay for Antigen Detection. |
| ULMB021Hu | 連環(huán)蛋白β1(β-catenin)等多因子檢測(cè)試劑盒(流式熒光發(fā)光法) | FLIA Kit for Antigen Detection. |
| UKSB021Hu01 | 連環(huán)蛋白β1(β-catenin)檢測(cè)試劑盒DIY材料(酶聯(lián)免疫吸附試驗(yàn)法) | 自己動(dòng)手制作ELISA試劑盒的主要材料 |
參考文獻(xiàn)
| 雜志 | 參考文獻(xiàn) |
| Clinica Chimica Acta | Versican and its associated molecules: Potential diagnostic markers for multiple myeloma [Pubmed:25623955] |
| Journal of Bone and Mineral Research | Adverse effects of osteocytic constitutive activation of ?-catenin on bone strength and bone growth [Pubmed:25639729] |
| Am J Transl Res | Role of SDF-1 and Wnt signaling pathway in the myocardial fibrosis of hypertensive rats [PubMed: 26396666] |
| Scientific Reports | Is there an association between enhanced choline and β-catenin pathway in breast cancer? A pilot study by MR Spectroscopy and ELISA. [pubmed:28533512] |
| Life Sciences | Hepatoprotective effect of hesperidin in hepatocellular carcinoma: Involvement of Wnt signaling pathways. [pubmed:28754618] |
| Scientific Reports | Is there an association between enhanced choline and β-catenin pathway in breast cancer? A pilot study by MR Spectroscopy and ELISA [Pubmed: 28533512] |
| The Showa University Journal of Medical Sciences | Eribulin Treatment Induces High Expression of miR-195 and Inactivates the Wnt/β-catenin Signaling Pathway in Triple-negative Breast Cancer [Doi: 10.15369/sujms.30.359] |
| Advances in?Medical?Sciences | Urinary procollagen III aminoterminal propeptide and β-catenin–New diagnostic biomarkers in solitary functioning kidney? [Pubmed: 30738287] |
| Biomed Pharmacother | Silence of FAM83H-AS1 promotes chemosensitivity of gastric cancer through Wnt/β-catenin signaling pathway [Pubmed: 32028241] |
| Journal of Cosmetic Dermatology | Novel complex of cosmetic ingredients with promising action in preventing hair loss and follicular aging through mechanism involving enrichment of WNT/signaling?… [Pubmed: 33179848] |
| Nutrients | Hydroxytyrosol as a Promising Ally in the Treatment of Fibromyalgia [Pubmed: 32784915] |
| Oncotarget | Amalgamation of PI3K and EZH2 blockade synergistically regulates invasion and angiogenesis: combination therapy for glioblastoma multiforme [33473259] |
| Cytokine | QNZ alleviated hepatocellular carcinoma by targeting inflammatory pathways in a rat model [34564023] |
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