神經(jīng)膠質(zhì)纖維酸性蛋白(GFAP)單克隆抗體
Monoclonal Antibody to Glial Fibrillary Acidic Protein (GFAP)
Intermediate Filament Protein
- 編號UMAA068Ra21
- 物種Rattus norvegicus (Rat，大鼠) 相同的名稱，不同的物種。
- 來源單抗制備
- 宿主小鼠
- 效價(克隆號)C1
- Ig亞型 IgG1 Kappa
- 純化方式蛋白A + 蛋白G親和純化
- 標記物無標記物
- 免疫原 RPA068Ra02-神經(jīng)膠質(zhì)纖維酸性蛋白(GFAP)重組蛋白
- 緩沖液成份0.01M 磷酸鹽緩沖液（pH7.4，containing 0.05% Proclin-300，50% glycerol）
- 性狀液體
- 濃度1mg/mL
- 且適物種Homo sapiens (Human，人), Sus scrofa; Porcine (Pig，豬)
- 應(yīng)用WB; IHC
如果抗體需用于流式細胞術(shù)，請參見流式抗體。 - 下載 英文說明書 中文說明書
- 規(guī)格 20μl100μl 200μl 1ml 10ml
- 價格 ¥ 326 ¥ 760 ¥ 1086 ¥ 2715 ¥ 10860
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產(chǎn)品包裝(模擬)
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產(chǎn)品包裝(模擬)
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DAB staining on IHC-P; Sample: Rat Cerebrum Tissue Primary Ab: 20μg/ml Mouse Anti-Rat GFAP Antibody Control: Used PBS instead of primary antibody Second Ab: 2μg/ml HRP-Linked Caprine Anti-Mouse IgG Polyclonal Antibody (Catalog: SAA544Mu19)
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Vector Red staining on IHC-P;
Samples: Human Brain Tissue;
Primary Ab: 10μg/ml Mouse Anti-Rat GFAP Antibody
Second Ab: 2μg/mL?HRP-Linked Caprine Anti-Mouse IgG Monoclonal Antibody
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Figure. Western Blot; Sample: Recombinant GFAP, Rat.
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Western Blot; Sample: Rat Cerebellum lysate;
Primary Ab: 2μg/ml Mouse Anti-Rat GFAP Antibody
Second Ab: 0.2μg/mL?HRP-Linked Caprine Anti-Mouse IgG Polyclonal Antibody
(Catalog: SAA544Mu19) -
Western Blot; Sample: Rat Cerebrum lysate;
Primary Ab: 2μg/ml Mouse Anti-Rat GFAP Antibody
Second Ab: 0.2μg/mL?HRP-Linked Caprine Anti-Mouse IgG Polyclonal Antibody
(Catalog: SAA544Mu19) -
Western Blot; Sample: U87MG cell lysate
Primary Ab: 0.1μg/ml Mouse Anti-Rat GFAP Antibody
Second Ab: 0.2μg/mL HRP-Linked Caprine Anti-Mouse IgG Polyclonal Antibody
(Catalog: SAA544Mu19) -
Western Blot; Samples: Lane1: Porcine Cerebrum lysate; Lane2: Porcine Cerebellum lysate;
Primary Ab: 0.1μg/ml Mouse Anti-Rat GFAP Antibody
Second Ab: 0.2μg/ml HRP-Linked Caprine Anti-Mouse IgG Polyclonal Antibody
(Catalog: SAA544Mu19)
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通過ISO 9001、ISO 13485質(zhì)量體系認證
特異性
該抗體是針對GFAP的小鼠單克隆抗體。在免疫組織化學(xué)染色和免疫印跡實驗中能識別GFAP。
用法
Western blotting: 0.01-2μg/mL;
Immunohistochemistry: 5-20μg/mL;
Optimal working dilutions must be determined by end user.
儲存
經(jīng)常使用則4°C保存。-20°C保存不超過兩年。避免反復(fù)凍融。
穩(wěn)定性
熱穩(wěn)定性以損失率顯示。損失率是由加速降解試驗決定，具體方法如下：在37°C孵育48小時，沒有顯著的降解或者沉淀產(chǎn)生。保質(zhì)期內(nèi)，在適當?shù)臈l件下存儲，損失率低于5%。
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參考文獻
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