甘油醛-3-磷酸脫氫酶(GAPDH)單克隆抗體
Monoclonal Antibody to Glyceraldehyde-3-Phosphate Dehydrogenase (GAPDH)
G3PD; GAPD; Peptidyl-cysteine S-nitrosylase GAPDH
- 編號(hào)UMAB932Hu27
- 物種Homo sapiens (Human，人) 相同的名稱，不同的物種。
- 來(lái)源單抗制備
- 宿主小鼠
- 效價(jià)(克隆號(hào))F2
- Ig亞型 IgG1 Kappa
- 純化方式蛋白A + 蛋白G親和純化
- 標(biāo)記物無(wú)標(biāo)記物
- 免疫原 RPB932Hu01-甘油醛-3-磷酸脫氫酶(GAPDH)重組蛋白
- 緩沖液成份0.01M 磷酸鹽緩沖液（pH7.4，containing 0.05% Proclin-300，50% glycerol）
- 性狀液體
- 濃度1mg/mL
- 且適物種Mus musculus (Mouse，小鼠), Rattus norvegicus (Rat，大鼠), Cavia (Guinea pig ，豚鼠), Oryctolagus cuniculus (Rabbit，兔), Sus scrofa; Porcine (Pig，豬), Ovis aries; Ovine (Sheep，綿羊), Chicken (Gallus，雞)
- 應(yīng)用WB; IHC
如果抗體需用于流式細(xì)胞術(shù)，請(qǐng)參見(jiàn)流式抗體。 - 下載 英文說(shuō)明書(shū) 中文說(shuō)明書(shū)
- 規(guī)格 20μl100μl 200μl 1ml 10ml
- 價(jià)格 ¥ 329 ¥ 767 ¥ 1095 ¥ 2738 ¥ 10950
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產(chǎn)品包裝(模擬)
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產(chǎn)品包裝(模擬)
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DAB staining on IHC-P; Sample: Human Endometrial cancer Tissue Primary Ab: 20μg/ml Mouse Anti-Human GAPDH Antibody Control: Used PBS instead of primary antibody Second Ab: 2μg/ml HRP-Linked Caprine Anti-Mouse IgG Polyclonal Antibody (Catalog: SAA544Mu19)
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DAB staining on IHC-P;
Sample: Human Lymphoma Tissue
Primary Ab: 20μg/ml Mouse Anti-Human GAPDH Antibody
Control: Used PBS instead of primary antibody
Second Ab: 2μg/ml HRP-Linked Caprine Anti-Mouse IgG Polyclonal Antibody
(Catalog: SAA544Mu19) -
DAB staining on IHC-P;
Sample: Human liver Cirrhosis Tissue
Primary Ab: 20μg/ml Mouse Anti-Human GAPDH Antibody
Control: Used PBS instead of primary antibody
Second Ab: 2μg/ml HRP-Linked Caprine Anti-Mouse IgG Polyclonal Antibody
(Catalog: SAA544Mu19)
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Western Blot; Sample: Rat Heart lysate Primary Ab: 1μg/ml Mouse Anti-Human GAPDH Antibody Second Ab: 0.2μg/ml HRP-Linked Caprine Anti-Mouse IgG Polyclonal Antibody (Catalog: SAA544Mu19)
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Western Blot; Samples: Lane1: Rat Cerebrum lysate; Lane2: Hela cell lysate;
Primary Ab: 1μg/ml Mouse Anti-Human GAPDH Antibody
Second Ab: 0.2μg/ml HRP-Linked Caprine Anti-Mouse IgG Polyclonal Antibody
(Catalog: SAA544Mu19) -
Western Blot; Samples: Lane1: Human Lung lysate; Lane2: 293T cell lysate; Lane3: Cavia Kidney lysate; Lane4: Porcine Liver lysate; Lane5: Porcine Kidney lysate;
Primary Ab: 0.5μg/ml Mouse Anti-Human GAPDH Antibody
Second Ab: 0.2μg/ml HRP-Linked Caprine Anti-Mouse IgG Polyclonal Antibody
(Catalog: SAA544Mu19) -
Western Blot; Sample: Cavia Liver lysate<br/>Primary Ab: 0.5μg/ml Mouse Anti-Human GAPDH Antibody<br/>Second Ab: 0.2μg/ml HRP-Linked Caprine Anti-Mouse IgG Polyclonal Antibody<br/>(Catalog: SAA544Mu19)
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Western Blot; Samples: Lane1: Gallus Kidney lysate; Lane2: Rabbit Liver lysate; Lane3: Rabbit Kidney lysate; Lane4: Ovine Liver lysate; Lane5: Mouse Liver lysate; Lane6: Rat Liver lysate; Lane7: Rat Kidney lysate;
Primary Ab: 0.5μg/ml Mouse Anti-Human GAPDH Antibody
Second Ab: 0.2μg/ml HRP-Linked Caprine Anti-Mouse IgG Polyclonal Antibody
(Catalog: SAA544Mu19)
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通過(guò)ISO 9001、ISO 13485質(zhì)量體系認(rèn)證
特異性
該抗體是針對(duì)GAPDH的小鼠單克隆抗體。在免疫組織化學(xué)染色和免疫印跡實(shí)驗(yàn)中能識(shí)別GAPDH。
用法
Western blotting: 0.01-2μg/mL;
Immunohistochemistry: 5-20μg/mL;
Immunocytochemistry: 5-20μg/mL;
Optimal working dilutions must be determined by end user.
儲(chǔ)存
經(jīng)常使用則4°C保存。-20°C保存不超過(guò)兩年。避免反復(fù)凍融。
穩(wěn)定性
熱穩(wěn)定性以損失率顯示。損失率是由加速降解試驗(yàn)決定，具體方法如下：在37°C孵育48小時(shí)，沒(méi)有顯著的降解或者沉淀產(chǎn)生。保質(zhì)期內(nèi)，在適當(dāng)?shù)臈l件下存儲(chǔ)，損失率低于5%。
贈(zèng)品
增值服務(wù)
抗體標(biāo)記定制服務(wù)
Protein-A/G純化柱
細(xì)胞和組織染色液
抗體陽(yáng)性對(duì)照品
組織/切片定制服務(wù)
磷酸化抗體定制服務(wù)
免疫印跡(WB)實(shí)驗(yàn)服務(wù)
免疫組織化學(xué)(IHC)實(shí)驗(yàn)服務(wù)
免疫細(xì)胞化學(xué)(ICC)實(shí)驗(yàn)服務(wù)
流式細(xì)胞術(shù)(FCM)實(shí)驗(yàn)服務(wù)
免疫沉淀(IP)實(shí)驗(yàn)服務(wù)
免疫熒光(IF)實(shí)驗(yàn)服務(wù)
緩沖液
DAB顯色試劑盒
SABC試劑盒
長(zhǎng)臂生物素標(biāo)記試劑盒
實(shí)時(shí)熒光定量PCR實(shí)驗(yàn)服務(wù)
相關(guān)產(chǎn)品
| 編號(hào) | 適用物種：Homo sapiens (Human，人) | 應(yīng)用(僅供研究使用，不用于臨床診斷！) |
| URPB932Hu02 | 甘油醛-3-磷酸脫氫酶(GAPDH)重組蛋白 | Positive Control; Immunogen; SDS-PAGE; WB. |
| URPB932Hu03 | 甘油醛-3-磷酸脫氫酶(GAPDH)重組蛋白 | Positive Control; Immunogen; SDS-PAGE; WB. |
| URPB932Hu01 | 甘油醛-3-磷酸脫氫酶(GAPDH)重組蛋白 | Positive Control; Immunogen; SDS-PAGE; WB. |
| UPAB932Hu01 | 甘油醛-3-磷酸脫氫酶(GAPDH)多克隆抗體 | WB; IHC; ICC/IF |
| UCAB932Hu01 | 抗甘油醛-3-磷酸脫氫酶(GAPDH)多克隆抗體 | WB; IHC |
| UPAB932Hu02 | 甘油醛-3-磷酸脫氫酶(GAPDH)多克隆抗體 | WB; IHC; ICC/IF; FCM |
| ULAB932Hu71 | 甘油醛-3-磷酸脫氫酶(GAPDH)多克隆抗體(生物素標(biāo)記) | WB; IHC; ICC. |
| UMAB932Hu24 | 甘油醛-3-磷酸脫氫酶(GAPDH)單克隆抗體 | IHC |
| UMAB932Hu25 | 甘油醛-3-磷酸脫氫酶(GAPDH)單克隆抗體 | WB; IHC |
| UMAB932Hu27 | 甘油醛-3-磷酸脫氫酶(GAPDH)單克隆抗體 | WB; IHC |
| UMAB932Hu29 | 甘油醛-3-磷酸脫氫酶(GAPDH)單克隆抗體 | WB; IHC |
| UMAB932Hu22 | 甘油醛-3-磷酸脫氫酶(GAPDH)單克隆抗體 | WB; IHC |
| UMAB932Hu26 | 甘油醛-3-磷酸脫氫酶(GAPDH)單克隆抗體 | WB |
| UMAB932Hu28 | 甘油醛-3-磷酸脫氫酶(GAPDH)單克隆抗體 | WB |
| UCAB932Hu22 | 抗甘油醛-3-磷酸脫氫酶(GAPDH)單克隆抗體 | WB; IHC; ICC/IF |
| UMAB932Hu21 | 甘油醛-3-磷酸脫氫酶(GAPDH)單克隆抗體 | WB; IHC; ICC/IF |
| UMAB932Hu23 | 甘油醛-3-磷酸脫氫酶(GAPDH)單克隆抗體 | WB |
| USEB932Hu | 甘油醛-3-磷酸脫氫酶(GAPDH)檢測(cè)試劑盒(酶聯(lián)免疫吸附試驗(yàn)法) | Enzyme-linked immunosorbent assay for Antigen Detection. |
| USCB932Hu | 甘油醛-3-磷酸脫氫酶(GAPDH)檢測(cè)試劑盒(化學(xué)發(fā)光免疫分析法) | Chemiluminescent immunoassay for Antigen Detection. |
| ULMB932Hu | 甘油醛-3-磷酸脫氫酶(GAPDH)等多因子檢測(cè)試劑盒(流式熒光發(fā)光法) | FLIA Kit for Antigen Detection. |
參考文獻(xiàn)
| 雜志 | 參考文獻(xiàn) |
| Clin Cancer Res | Inactivation of the p53–KLF4–CEBPA Axis in Acute Myeloid Leukemia [PubMed: 26408402] |
| Clin Cancer Res | Inactivation of the p53–KLF10–CEBPA Axis in Acute Myeloid Leukemia [PubMed: 26408402] |
| Diabetes & Metabolism | Dapagliflozin modulates glucagon secretion in an SGLT2-independent manner in murine alpha cells. [pubmed:28499695] |
| Scientific Reports | Adhesion of Toxoplasma gondii tachyzoite-infected vehicle leukocytes to capillary endothelial cells triggers timely parasite egression [pubmed:28720868] |
| Journal of Biological Chemistry | Biochemical characterization of the PHARC associated serine hydrolase ABHD12 reveals its preference for very long chain lipids [Pubmed: 30237167] |
| Biomaterials | Untangling the response of bone tumor cells and bone forming cells to matrix stiffness and adhesion ligand density by means of hydrogels [Pubmed: 30343256] |
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| Cancers (Basel) | BMI1-Inhibitor PTC596 in Combination with MCL1 Inhibitor S63845 or MEK Inhibitor Trametinib in the Treatment of Acute Leukemia. Cancers 2021, 13, 581 [33540760] |
| MOLECULAR CANCER RESEARCH | Omega-3 Eicosapentaenoic Acid Reduces Prostate Tumor Vascularity [33262291] |
| Sci Rep | Curcumin derivative ST09 modulates the miR-199a-5p/DDR1 axis and regulates proliferation and migration in ovarian cancer cells [34837026] |
| Cancer Rep (Hoboken) | Curcumin alters distinct molecular pathways in breast cancer subtypes revealed by integrated miRNA/mRNA expression analysis [Pubmed:34981672] |
| Prog Neurobiol | Retinoic acid receptor beta protects striatopallidal medium spiny neurons from mitochondrial dysfunction and neurodegeneration [Pubmed:35151792] |
| Plant Molecular Biology | Arabidopsis GELP7 functions as a plasma membrane-localized acetyl xylan esterase, and its overexpression improves saccharification efficiency [Pubmed:35577991] |
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