輕肽神經(jīng)絲蛋白(NEFL)單克隆抗體
Monoclonal Antibody to Neurofilament, Light Polypeptide (NEFL)
CMT1F; CMT2E; NF-L; NF68; NFL; 68 kDa neurofilament protein; Neurofilament triplet L protein
- 編號UMAE038Hu21
- 物種Homo sapiens (Human，人) 相同的名稱，不同的物種。
- 來源單抗制備
- 宿主小鼠
- 效價(克隆號)C4
- Ig亞型 IgG1 Kappa
- 純化方式蛋白A + 蛋白G親和純化
- 標(biāo)記物無標(biāo)記物
- 免疫原 RPE038Hu02-輕肽神經(jīng)絲蛋白(NEFL)重組蛋白
- 緩沖液成份磷酸鹽緩沖液（pH7.4,含有0.02% NaN3和50%甘油）
- 性狀液體
- 濃度1mg/ml
- 且適物種Mus musculus (Mouse，小鼠), Rattus norvegicus (Rat，大鼠), Sus scrofa; Porcine (Pig，豬)
- 應(yīng)用WB; IHC; FCM
如果抗體需用于流式細(xì)胞術(shù)，請參見流式抗體。 - 下載 英文說明書 中文說明書
- 規(guī)格 20μl100μl 200μl 1ml 10ml
- 價格 ¥ 333 ¥ 777 ¥ 1110 ¥ 2775 ¥ 11100
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產(chǎn)品包裝(模擬)
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產(chǎn)品包裝(模擬)
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DAB staining on IHC-P; Sample: Rat Cerebrum Tissue; Primary Ab: 20ug/ml Mouse Anti-Human NEFL Antibody Second Ab: 2μg/mL?HRP-Linked Caprine Anti-Mouse IgG Polyclonal Antibody (Catalog: SAA544Mu19)
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DAB staining on IHC-P;
Sample: Rat Spinal cord Tissue;
Primary Ab: 20ug/ml Mouse Anti-Human NEFL Antibody
Second Ab: 2μg/mL?HRP-Linked Caprine Anti-Mouse IgG Polyclonal Antibody
(Catalog: SAA544Mu19) -
DAB staining on IHC-P;
Sample: Rat Cerebellum Tissue;
Primary Ab: 20ug/ml Mouse Anti-Human NEFL Antibody
Second Ab: 2μg/mL?HRP-Linked Caprine Anti-Mouse IgG Polyclonal Antibody
(Catalog: SAA544Mu19)
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Western Blot; Sample: Lane1: Porcine Cerebrum lysate; Lane2: Rat Cerebrum lysate; Lane3: Mouse Cerebrum lysate Primary Ab: 0.001μg/ml Mouse Anti-Human NEFL Antibody Second Ab: 0.2μg/mL HRP-Linked Caprine Anti-Mouse IgG Polyclonal Antibody (Catalog: SAA544Mu19)
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Human SH-SY5Y cell line was fixed with 2% paraformaldehyde (10 min) , permeabilised with 0.2% BSA-Triton X-100,then stained with 10μg/ml mouse Anti-human NEFL Monoclonal Antibody (Catalog MAE038Hu21, red histogram) or Isotype control antibody (Catalog IS067-Mu01, green histogram), followed by 1μg/ml FITC-conjugated Anti-mouse IgG Secondary Antibody (Catalog SAA544Mu18).
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通過ISO 9001、ISO 13485質(zhì)量體系認(rèn)證
特異性
該抗體是針對NEFL的小鼠單克隆抗體。在免疫組織化學(xué)染色和免疫印跡實驗中能識別NEFL。
用法
Western blotting: 0.001-2μg/mL;
Immunohistochemistry: 5-20μg/mL;
Flow cytometry: 10μg/ml;
Optimal working dilutions must be determined by end user.
儲存
經(jīng)常使用則4°C保存。-20°C保存不超過兩年。避免反復(fù)凍融。
穩(wěn)定性
熱穩(wěn)定性以損失率顯示。損失率是由加速降解試驗決定，具體方法如下：在37°C孵育48小時，沒有顯著的降解或者沉淀產(chǎn)生。保質(zhì)期內(nèi)，在適當(dāng)?shù)臈l件下存儲，損失率低于5%。
贈品
增值服務(wù)
相關(guān)產(chǎn)品
| 編號 | 適用物種：Homo sapiens (Human，人) | 應(yīng)用(僅供研究使用，不用于臨床診斷！) |
| URPE038Hu03 | 輕肽神經(jīng)絲蛋白(NEFL)重組蛋白 | Positive Control; Immunogen; SDS-PAGE; WB. |
| URPE038Hu02 | 輕肽神經(jīng)絲蛋白(NEFL)重組蛋白 | Positive Control; Immunogen; SDS-PAGE; WB. |
| URPE038Hu01 | 輕肽神經(jīng)絲蛋白(NEFL)重組蛋白 | Positive Control; Immunogen; SDS-PAGE; WB. |
| UCPE038Hu21 | 輕肽神經(jīng)絲蛋白(NEFL)卵白蛋白偶聯(lián)物 | Immunogen; SDS-PAGE; WB. |
| UPAE038Hu08 | 輕肽神經(jīng)絲蛋白(NEFL)多克隆抗體 | WB; IHC; ICC; IP. |
| UPAE038Hu02 | 輕肽神經(jīng)絲蛋白(NEFL)多克隆抗體 | WB; IHC; ICC/IF |
| UPAE038Hu01 | 輕肽神經(jīng)絲蛋白(NEFL)多克隆抗體 | WB |
| ULAE038Hu71 | 輕肽神經(jīng)絲蛋白(NEFL)多克隆抗體(生物素標(biāo)記) | WB; IHC; ICC. |
| UMAE038Hu25 | 輕肽神經(jīng)絲蛋白(NEFL)單克隆抗體 | ICC/IF |
| UMAE038Hu27 | 輕肽神經(jīng)絲蛋白(NEFL)單克隆抗體 | ICC/IF; FCM |
| UMAE038Hu23 | 輕肽神經(jīng)絲蛋白(NEFL)單克隆抗體 | WB |
| UMAE038Hu22 | 輕肽神經(jīng)絲蛋白(NEFL)單克隆抗體 | WB; IHC |
| UMAE038Hu21 | 輕肽神經(jīng)絲蛋白(NEFL)單克隆抗體 | WB; IHC; FCM |
| UMAE038Hu24 | 輕肽神經(jīng)絲蛋白(NEFL)單克隆抗體 | WB; FCM |
| UFAE038Hu08 | 抗輕肽神經(jīng)絲蛋白(NEFL)單克隆抗體 | FCM |
| UFAE038Hu02 | 抗輕肽神經(jīng)絲蛋白(NEFL)單克隆抗體 | FCM |
| UFAE038Hu06 | 抗輕肽神經(jīng)絲蛋白(NEFL)單克隆抗體 | FCM |
| UFAE038Hu04 | 抗輕肽神經(jīng)絲蛋白(NEFL)單克隆抗體 | FCM |
| USEE038Hu | 輕肽神經(jīng)絲蛋白(NEFL)檢測試劑盒(酶聯(lián)免疫吸附試驗法) | Enzyme-linked immunosorbent assay for Antigen Detection. |
| UHEE038Hu | 輕肽神經(jīng)絲蛋白(NEFL)檢測試劑盒(酶聯(lián)免疫吸附試驗法,高敏型) | Enzyme-linked immunosorbent assay for Antigen Detection. |
| UMEE038Hu | 輕肽神經(jīng)絲蛋白(NEFL)檢測試劑盒(酶聯(lián)免疫吸附試驗法,小樣本) | Enzyme-linked immunosorbent assay for Antigen Detection. |
| UCEE038Hu | 輕肽神經(jīng)絲蛋白(NEFL)檢測試劑盒(酶聯(lián)免疫吸附試驗法) | Enzyme-linked immunosorbent assay for Antigen Detection. |
| USCE038Hu | 輕肽神經(jīng)絲蛋白(NEFL)檢測試劑盒(化學(xué)發(fā)光免疫分析法) | Chemiluminescent immunoassay for Antigen Detection. |
| ULME038Hu | 輕肽神經(jīng)絲蛋白(NEFL)等多因子檢測試劑盒(流式熒光發(fā)光法) | FLIA Kit for Antigen Detection. |
| UKSE038Hu01 | 輕肽神經(jīng)絲蛋白(NEFL)檢測試劑盒DIY材料(酶聯(lián)免疫吸附試驗法) | Main materials for "Do It (ELISA Kit) Yourself". |
參考文獻(xiàn)
| 雜志 | 參考文獻(xiàn) |
| Acta Neurologica Scandinavica | Effect of carotid endarterectomy on brain damage markers [Pubmed:27126899] |
| Acta Neurologica Scandinavica | Effect of carotid endarterectomy on brain damage markers. [pubmed:27126899] |
| journal of neuroimmunology | Cytokines and biological markers in autoimmune GFAP astrocytopathy: The potential role for pathogenesis and therapeutic implications [Pubmed: 31254929] |
| Cerebellum | Neurofilament Light Chain Is a Biomarker of Neurodegeneration in Ataxia Telangiectasia [33893614] |
| Medicine (Baltimore) | Phase 1b dose-escalation, safety, and pharmacokinetic study of IC14, a monoclonal antibody against CD14, for the treatment of amyotrophic lateral sclerosis [34678870] |
| J Clin Med | Fatty Acid-Binding Protein 7 (FABP-7), Glutamic Acid and Neurofilament Light Chain (NFL) as Potential Markers of Neurodegenerative Disorders in Psoriatic Patients?… [Pubmed:35566558] |
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