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70kDa熱休克蛋白1A(HSPA1A)多克隆抗體

Polyclonal Antibody to Heat Shock 70kDa Protein 1A (HSPA1A)

HSP70-1; HSP-A1A; HSPA1; HSP70-2; HSX70

編號UPAB081Hu02
物種Homo sapiens (Human，人) 相同的名稱，不同的物種。
- 所有
- 人
- 小鼠
- 大鼠
- 豚鼠
- 家兔
- 獼猴
- 山羊
- 綿羊
- 馬
- 牛
- 豬
- 雞
- 狗
- 其他物種
- 多物種
- 泛物種
來源多抗制備
宿主兔
效價-
Ig類型 IgG
純化方式抗原特異性親和純化
標記物無標記物
免疫原 RPB081Hu02-70kDa熱休克蛋白1A(HSPA1A)重組蛋白
緩沖液成份0.01M 磷酸鹽緩沖液（pH7.4，containing 0.05% Proclin-300，50% glycerol）
性狀液體
濃度500μg/mL
且適物種Mus musculus (Mouse，小鼠), Sus scrofa; Porcine (Pig，豬)
應用WB; IHC; ICC/IF
如果抗體需用于流式細胞術(shù)，請參見流式抗體。
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規(guī)格 20μl100μl 200μl 1ml 10ml
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產(chǎn)品包裝(模擬)
產(chǎn)品包裝(模擬)

DAB staining on IHC-P; Samples: Human Stomach Tissue; Primary Ab: 10μg/ml Rabbit Anti-Human HSPA1A Antibody Second Ab: 2μg/mL?HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody (Catalog: SAA544Rb19)
DAB staining on IHC-P;
Samples: Human Brain Tissue;
Primary Ab: 10μg/ml Rabbit Anti-Human HSPA1A Antibody
Second Ab: 2μg/mL?HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
(Catalog: SAA544Rb19)
DAB staining on IHC-P;
Samples: Human Prostate Tissue;
Primary Ab: 10μg/ml Rabbit Anti-Human HSPA1A Antibody
Second Ab: 2μg/mL?HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
(Catalog: SAA544Rb19)
DAB staining on IHC-P;
Samples: Human Kidney Tissue;
Primary Ab: 10μg/ml Rabbit Anti-Human HSPA1A Antibody
Second Ab: 2μg/mL?HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
(Catalog: SAA544Rb19)
DAB staining on IHC-P;
Samples: Human Breast cancer Tissue;
Primary Ab: 10μg/ml Rabbit Anti-Human HSPA1A Antibody
Second Ab: 2μg/mL?HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
(Catalog: SAA544Rb19)
DAB staining on IHC-P;
Samples: Human Prostate cancer Tissue;
Primary Ab: 10μg/ml Rabbit Anti-Human HSPA1A Antibody
Second Ab: 2μg/mL?HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
(Catalog: SAA544Rb19)
AF488 staining on IF;
Sample: Hela cell
Primary Ab: 20μg/ml Rabbit Anti-Human HSPA1A Antibody
Second Ab: 2μg/ml AF488-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
(Catalog: SAA544Rb11)

Figure. Western Blot; Sample: Recombinant HSPA1A, Human.
Western Blot; Sample: Lane1: Porcine Liver lysate; Lane2: Porcine Cerebrum lysate; Lane3: Mouse Liver lysate; Lane4: A549 cell lysate
Primary Ab: 3μg/ml Rabbit Anti-Human HSPA1A Antibody
Second Ab: 0.2μg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
(Catalog: SAA544Rb19)
Knockout Varification: Lane 1: Wild-type A549 cell lysate; Lane 2: HSPA1A knockout A549 cell lysate; Predicted MW: 64,70kDa Observed MW: 64kDa Primary Ab: 3μg/ml Rabbit Anti-Human HSPA1A Antibody Second Ab: 0.2μg/mL?HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody (Catalog: SAA544Rb19)
Western Blot; Samples: Lane1: Mouse Liver lysate; Lane2: Hela cell lysate; Lane3: A549 cell lysate;
Primary Ab: 0.1μg/ml Rabbit Anti-Human HSPA1A Antibody
Second Ab: 0.2μg/ml HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
(Catalog: SAA544Rb19)

通過ISO 9001、ISO 13485質(zhì)量體系認證

特異性

該抗體是針對HSPA1A的兔多克隆抗體。在免疫組織化學染色和免疫印跡實驗中能識別HSPA1A。

用法

Western blotting: 0.5-3μg/mL;
Immunohistochemistry: 5-20μg/mL;
Immunocytochemistry: 5-20μg/mL;
Optimal working dilutions must be determined by end user.

儲存

經(jīng)常使用則4°C保存。-20°C保存不超過兩年。避免反復凍融。

穩(wěn)定性

熱穩(wěn)定性以損失率顯示。損失率是由加速降解試驗決定，具體方法如下：在37°C孵育48小時，沒有顯著的降解或者沉淀產(chǎn)生。保質(zhì)期內(nèi)，在適當?shù)臈l件下存儲，損失率低于5%。

贈品

增值服務

抗體標記定制服務
 Protein-A/G純化柱
 細胞和組織染色液
 抗體陽性對照品
 組織/切片定制服務
 磷酸化抗體定制服務
 免疫印跡(WB)實驗服務
 免疫組織化學(IHC)實驗服務
 免疫細胞化學(ICC)實驗服務
 流式細胞術(shù)(FCM)實驗服務
 免疫沉淀(IP)實驗服務
 免疫熒光(IF)實驗服務
 緩沖液
 DAB顯色試劑盒
 SABC試劑盒
 長臂生物素標記試劑盒
 實時熒光定量PCR實驗服務

相關(guān)產(chǎn)品

編號	適用物種：Homo sapiens (Human，人)	應用(僅供研究使用，不用于臨床診斷！)
URPB081Hu02	70kDa熱休克蛋白1A(HSPA1A)重組蛋白	Positive Control; Immunogen; SDS-PAGE; WB.
URPB081Hu01	70kDa熱休克蛋白1A(HSPA1A)重組蛋白	Positive Control; Immunogen; SDS-PAGE; WB.
UCAB081Hu01	抗70kDa熱休克蛋白1A(HSPA1A)多克隆抗體	WB; IHC
UPAB081Hu01	70kDa熱休克蛋白1A(HSPA1A)多克隆抗體	WB; IHC; ICC/IF; FCM
UPAB081Hu02	70kDa熱休克蛋白1A(HSPA1A)多克隆抗體	WB; IHC; ICC/IF
UFAB081Hu03	抗70kDa熱休克蛋白1A(HSPA1A)多克隆抗體	FCM
UMAB081Hu21	70kDa熱休克蛋白1A(HSPA1A)單克隆抗體	WB; IHC; ICC/IF
UMAB081Hu29	70kDa熱休克蛋白1A(HSPA1A)單克隆抗體	WB
UMAB081Hu25	70kDa熱休克蛋白1A(HSPA1A)單克隆抗體	WB
UMAB081Hu23	70kDa熱休克蛋白1A(HSPA1A)單克隆抗體	WB
UMAB081Hu24	70kDa熱休克蛋白1A(HSPA1A)單克隆抗體	WB
UMAB081Hu28	70kDa熱休克蛋白1A(HSPA1A)單克隆抗體	WB
UMAB081Hu26	70kDa熱休克蛋白1A(HSPA1A)單克隆抗體	WB
UMAB081Hu27	70kDa熱休克蛋白1A(HSPA1A)單克隆抗體	WB
UCAB081Hu22	抗70kDa熱休克蛋白1A(HSPA1A)單克隆抗體	WB; IHC; ICC/IF
UMAB081Hu22	70kDa熱休克蛋白1A(HSPA1A)單克隆抗體	WB
UFAB081Hu02	抗70kDa熱休克蛋白1A(HSPA1A)單克隆抗體	FCM
UHEB081Hu	70kDa熱休克蛋白1A(HSPA1A)檢測試劑盒(酶聯(lián)免疫吸附試驗法,高敏型)	Enzyme-linked immunosorbent assay for Antigen Detection.
USEB081Hu	70kDa熱休克蛋白1A(HSPA1A)檢測試劑盒(酶聯(lián)免疫吸附試驗法)	Enzyme-linked immunosorbent assay for Antigen Detection.
ULMB081Hu	70kDa熱休克蛋白1A(HSPA1A)等多因子檢測試劑盒(流式熒光發(fā)光法)	FLIA Kit for Antigen Detection.
UKSB081Hu01	70kDa熱休克蛋白1A(HSPA1A)檢測試劑盒DIY材料(酶聯(lián)免疫吸附試驗法)	Main materials for "Do It (ELISA Kit) Yourself".
UCGB081Hu01	70kDa熱休克蛋白1A(HSPA1A)cDNA	PCR、蛋白表達等

參考文獻

雜志	參考文獻
Journal of Neuroinflammation	Identification of potential biomarkers of gold nanoparticle toxicity in rat brains [PubMed: 22691312]
Mediators Inflamm.?	Local Inflammation in Fracture Hematoma: Results from a Combined Trauma Model in Pigs [Pubmed:Pmc4324980]
Eur J Pharmacol.	Differential modulatory effects of morphine on acute and chronic stress induced neurobehavioral and cellular markers in rats [Pubmed:24508522]
Pharmacol Rep.	Effect of propranolol on IL-10, visfatin, Hsp70, iNOS, TLR2, and survivin in amelioration of tumor progression and survival in Solid Ehrlich Carcinoma-bearing mice [Pubmed:25443743]
Advances in Medical Sciences	A similar pro/anti-inflammatory cytokine balance is present in the airways of competitive athletes and non-exercising asthmatics [pubmed:28822267]
Archives of Medical Research	Winter ambient training conditions are associated with increased bronchial hyperreactivity and with shifts in serum innate immunity proteins in young?… [Pubmed:29379533]
metabolic brain disease	Curcumin attenuates cytoplasmic/endoplasmic reticulum stress, apoptosis and cholinergic dysfunction in diabetic rat hippocampus [Pubmed: 32172517]
EXPERIMENTAL CELL RESEARCH	Antagomirs targeting miR-142–5p attenuate pilocarpine-induced status epilepticus in mice [Pubmed: 32439493]

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